Identification of genetic variation in the major histocompatibility complex gene region in Turkish sheep breeds

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dc.contributor.authorIlhan, F.
dc.contributor.authorKeskin, I.
dc.contributor.authorTozluca, A.
dc.date.accessioned2020-03-26T19:24:35Z
dc.date.available2020-03-26T19:24:35Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe major histocompatibility complex (MHC) in sheep, Ovar-Mhc, remains poorly characterized relative to other domestic animals. However, its basic structure is similar to that of other mammals, comprising class I, II and III regions. In this study, the Ovine MHC class II DRB1 and DRB3 genes were amplified by polymerase chain reaction in eight sheep breeds reared in Turkey. Informative restriction fragment length polymorphisms (RFLPs) were obtained with five restriction enzymes for DRB1 and with two restriction enzymes for DRB3. The digestion of DRB1 exon 2 with NciI, SacI, SacII, Hin1I each produced three genotypes and two alleles (viz., a and b) with frequencies of 0.69 and 0.31; 0.65 and 0.35; 0.91 and 0.09; 0.57 and 0.43, respectively. The digestion of DRB1 exon 2 with DdeI produced four genotypes and three alleles (viz., a, b and c) with frequencies of 0.62, 0.28 and 0.10, respectively. On the other hand, the digestion of DRB3 exon 2 with NdeII and BsaI each produced three genotypes and two alleles (viz., a and b) with frequencies of 0.72 and 0.28; 0.96 and 0.04, respectively. This study presents the genetic profiles of the exon 2 region of the MHC DRB1 and DRB3 genes in native Turkish sheep breeds.en_US
dc.description.sponsorshipScientific Research Project Coordinating Office of Selcuk University [10101036]en_US
dc.description.sponsorshipThis study was performed by Fatma Ilhan in partial fulfilment of the Ph.D. degree in Biometry and Genetics, Animal Science Dept., Selcuk University. This project was supported by a grant from The Scientific Research Project Coordinating Office of Selcuk University. (Project No: 10101036).en_US
dc.identifier.doi10.4314/sajas.v46i4.4en_US
dc.identifier.endpage372en_US
dc.identifier.issn0375-1589en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage366en_US
dc.identifier.urihttps://dx.doi.org/10.4314/sajas.v46i4.4
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33686
dc.identifier.volume46en_US
dc.identifier.wosWOS:000390339200004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSOUTH AFRICAN JOURNAL OF ANIMAL SCIENCESen_US
dc.relation.ispartofSOUTH AFRICAN JOURNAL OF ANIMAL SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDRB1en_US
dc.subjectDRB3en_US
dc.subjectpolymerase chain reaction (PCR)en_US
dc.subjectrestriction fragment length polymorphism (RFLP)en_US
dc.titleIdentification of genetic variation in the major histocompatibility complex gene region in Turkish sheep breedsen_US
dc.typeArticleen_US

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