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Öğe Akut Yüzme Egzersizi Yaptırılan Ratlarda Çinko Eksikliği ve Uygulamasının Bazı Hematolojik Parametrelere Etkisi(2002) Baltacı, Abdülkerim Kasım; Özyürek, Kürşat; Moğulkoç, Rasim; Kurtoğlu, Erdal; Öztekin, Esma; Kul, AylinAmaç: Bu çalışma akut yüzme egzersizi yaptırılan ratlarda çinko eksikliğinin ve çinko uygulamasının bazı hematolojik parametreleri nasıl etkilediğinin ortaya konulabilmesi amacıyla gerçekleştirildi. Yöntem: Araştırma çinko eksik yüzme grubu (grup I, n10), çinko takviyeli yüzme grubu (grup II, n10), yüzme kontrol grubu (grup III, n10), genel kontrol grubu (grup IV, n10) olmak üzere toplam 40 adet Spraque-Dawley cinsi erişkin erkek rat üzerinde gerçekleştirildi. Dört hafta süren çalışmanın bitiminde deney hayvanlarından dekapitasyonla alınan kan örneklerinde eritrosit, hemoglobin, hematokrit, lökosit, lenfosit, trombosit parametreleri (otomatik kan sayım cihazı ile) ve plazma çinko düzeyleri (atomik absorpsiyon spektrofotometresi ile) tayin edildi. Bulgular: Grup 1'in eritrosit, Hb, Hct ve lenfosit değerleri en düşük, Grup 2'nin aynı değerleri en yüksekti. Grup 2'nin trombosit sayısı diğer gruplardan düşüktü. Sonuç: Bulgularımız çinko eksikliğinin belirtilen parametreleri olumsuz, çinko uygulamasının ise olumlu olarak etkilediğini düşündürmektedir. Sonuç olarak çinko uygulaması fiziksel performansı artırıcı yönde etki gösterebilir.Öğe Characterization of T Lymphocyte Subtypes in Endotoxin-İnduced Uveitis and Effect of Pentoxifylline Treatment(Swets Zeitlinger Publishers, 2002) Avunduk, Avni Murat; Avunduk, Mustafa Cihat; Öztekin, Esma; Baltacı, Abdulkerim KasımPurpose. The aims of the study were twofold: 1) to investigate the role of T lymphocyte subtypes in the pathogenesis of endotoxin-induced uveitis (EIU) and 2) to study the possible beneficial effect of pentoxifylline, an inhibitor of neutrophil motility, and Tumor Necrosis Factora-alpha on this disease. Methods. Forty-two inbred male Lewis rats were divided into seven equal groups. 200 m g of Escherichia coli 055: B55 lipopolysaccharide (LPS) was injected in one hind footpad of the Group 2, 3, 4, 5, 6, and 7 rats. Group 5, 6, and 7 rats also received concomitant intraperitoneal pentoxifylline (PTX) during food pad injection of LPS. Group 1 rats were used as controls with intra-peritoneal normal saline injection. Eight, 24, and 48 hours after treatment, the rats were euthanized. Neutrophil leukocyte, mononuclear cells, and CD4+, CD8+, and CD45RA+ cell infiltration in the anterior uveal tissue were determined either by hematoxylin-eosin or monoclonal antibody staining. Tumor Necrosis Factor-alpha (TNF-alpha) levels were also measured in the aqueous and blood samples. We compared the numbers of infiltrating cells in the different groups. Results. We found that peak infiltration of lymphocyte, neutrophils, and CD4+ cells occurred at 24 hours. However, CD8+ and CD45RA+ cell number reached their highest levels at 48 hours. There was no inflammatory cell infiltration in the control rats. Concomitant pentoxifylline treatment did not affect any of these parameters, although it effectively reduced TNF-alpha concentrations in the anterior chamber and the serum. Conclusion. We conclude that, 1) T lymphocytes might be involved in the pathogenesis of endotoxin-induced uveitis. 2) The potential role of pentoxifylline in the treatment of human uveitis is questionable. However, these are initial findings and need confirmation by additional studies.Öğe Effect of pinealectomy on homocysteine levels in ovariectomized rats(MAGHIRA & MAAS PUBLICATIONS, 2007) Öztekin, Esma; Öztürk, Bahadır; Moğulkoç, Rasim; Baltacı, Abdülkerim Kasım; Tiftik, Ali MuhtarOBJECTIVE: Hyperhomocysteinemia plays a role in obstetric and gynecological diseases and such diseases are seen as a consequence of an impaired methionine cycle. In the present study, it was aimed to determine the effect of estradiol and progesterone on homocysteine levels in pinealectomized and ovariectomized rats. MATERIALS AND METHODS: The study was carried out on 24 adult Spraque-Dawley female rats. Rats were divided into 4 groups as follows: Group 1: Sham ovariectomy group (Sham-Ovx), Group 2: Ovariectomized and sham pinealectomized group (Ovx-Sham-Px), Group 3: Ovariectomized and pinealectomized group (Ovx-Px), Group 4: Group which were supplemented with estradiol and progesterone after ovariectomy and pinealectomy (Ovx-Px-E+P). Serum homocysteine levels were determined after experimental period. RESULTS: Homocysteine levels in Group 3 were higher than those in Groups 1, 2 and 4 (p<0.05). Homocysteine levels in Groups 2 and 4 were higher than those in Group 1 (p<0.05). Homocysteine levels in Groups 2 and 4 were not different. CONCLUSION: The findings of the present study demonstrate that ovariectomy lead to increases homocysteine levels. Pinealectomy in addition to ovariectomy increases homocysteine levels. However, administration of estradiol and progesterone following pinealectomy prevents the increase in homocysteine levels in ovariectomized rats.Öğe Effects of Aminoglycoside Antibiotics on Renal Antioxidants, Malondialdehyde Levels, and Some Serum Biochemical Parameters(Canadian Vet Med Assoc, 2003) Yazar, Enver; Elmas, Muammer; Altunok, Vahdettin; Sivrikaya, Abdullah; Öztekin, Esma; Birdane, Yavuz OsmanEffects of amikacin, gentamicin, kanamycin, and streptomycin on renal tissue superoxide dismutase, glutathione peroxidase, glutathione and malondialdehyde, serum creatinine, potassium, sodium, total protein, glucose, uric acid, and total bilirubin levels were investigated. All aminoglycoside antibiotics decreased renal tissue glutathione levels.Öğe The effects of boron administration on plasma leptin and lactate levels in ovariectomized rats which had acute swimming exercise(MAGHIRA & MAAS PUBLICATIONS, 2008) Yazıcı, Zübeyde; Kaya, Yalçın; Baltacı, Abdülkerim Kasım; Moğulkoç, Rasim; Öztekin, EsmaOBJECTIVES: The aim of this study was to investigate the effects of intraperitoneal (IP) boron administration on leptin and lactate levels in swimming exercise in ovariectomized rats. METHODS: Eighty adult female rats were equally allocated into 8 groups. Group 1; Control, Group 2; exercise control, Group 3; ovariectomized control, Group 4; Boron control (2 mg/kg/day), Group 5; ovariectomized plus exercise, Group 6; exercise plus IP boron, Group 7; ovariectomized and exercise group plus IP boron, Group 8; ovariectomized plus boron. RESULTS: Leptin levels in Group I were higher than those in Groups 2, 3 and 4 and lower than those in groups 5, 6, 7 and 8 (p<0.01). Leptin levels were higher in Group 3 than in Groups 2 and 4 and significantly lower than in all other groups (p<0.01). Lactate levels in Groups 2 and 4 were higher than those in all other groups (p<0.01). Lactate levels were significantly lower in Groups 1, 3, 5 and 8 than in all other groups (p<0.01). Lactate levels in Groups 6 and 7 were significantly lower than those in Groups 2 and 4 (p<0.01) and higher than those in groups 1, 3 and 5 (P<0.01). CONCLUSION: The present findings demonstrate that ovariectomy and acute swimming exercise in rats led to a significant decrease in leptin levels and a significant increase in lactate levels and that boron administration prevented the increased in circulating lactate concentration induced by swimming exercise.Öğe The effects of exogenous estradiol and progesterone with melatonin on homocysteine levels in ovariectomized rats(INDIAN VETERINARY JOURNAL, 2007) Öztekin, Esma; Moğulkoç, Rasim; Baltacı, Abdülkerim Kasım; Öztürk, Bahadır; Tiftik, Ali MuhtarHomocysteine, a product of methionine cycle, plays a role in obstetric and gynaecological diseases and these diseases are considered as a result of impaired methionine cycle (Obsweger et al., 1994). Estrogen is considered a nongenetic factor influencing homocysteine metabolism (Pines et al., 1997) Homocysteine levels can be a risk factor for arteriosclerosis and osteoporosis (El-Swefy et al., 2002). El-Swefy et al., (loc. cit) found that high homocysteine levels in ovariectomized rats decreased with estradiol and folic acid supplementation. The aim of this study was to investigate effect of replacement of estradiol, progesterone and melatonin on homocysteine levels in ovariectomized rats.Öğe Effects of Hyperthyroidism Induced by L-Thyroxin Administration on Lipid Peroxidation in Various Rat Tissues(Akademiai Kiado, 2006) Moğulkoç, R.; Baltacı, A. Kasım; Öztekin, Esma; Sivrikaya, A.; Aydın, LeylaThyroid dysfunctions are associated with many pathological signs in the body. One of these is lipid peroxidation that develops due to over- or under-secretion of thyroid hormones. The present study was conducted to determine lipid peroxidation that develops in different tissues including the brain, liver and heart of rats in experimental hyperthyroidism induced by L-thyroxin. The study was carried out on 30 male Sprague-Dawley rats. They were divided into three groups as control, sham hyperthyroidism and hyperthyroidism. Malondialdehyde (MDA) and glutathione (GSH) levels in rat tissues were determined at the end of a 3-weeks period of L-thyroxin administration. It was observed that MDA levels in the hyperthyroidism group were significantly higher in the cerebral cortex, liver and ventriculer tissue of heart (p < 0.001) than in the control and in sham hyperthyroidism groups. GSH levels were higher in the hyperthyroidism group than in control and sham hyperthyroidism groups in all tissues (p < 0.001). Results demonstrate that hyperthyroidism induced by L-thyroxin activates both oxidant and antioxidant systems in cerebral, hepatic and cardiac tissues. However, the increase in antioxidant activity cannot adequately prevent oxidative damage.Öğe Effects of Zinc Deficiency and Supplementation on Malondialdehyde and Glutathione Levels in Blood and Tissues of Rats Performing Swimming Exercise(Humana Press Inc, 2003) Öztürk, Ahmet; Baltacı, Abdülkerim Kasım; Moğulkoç, Rasim; Öztekin, Esma; Sivrikaya, Abdullah; Kurtoğlu, Erdal; Kul, AylinThe aim of the study was to investigate the effects of zinc deficiency and supplementation on lipid peroxidation and glutathione levels in blood and in some tissues of rats performing swimming exercise. Forty adult male Sprague-Dawley rats were divided into four groups: group 1, zinc-deficient consisted of swimming rats; group 2 consisted of zinc-supplemented swimming rats; groups 3 and 4 were the swimming and nonswimming controls, respectively. The levels of malondialdehyde and glutathione were measured after 4 wk of zinc-deficient or zinc-supplemented diet and 30 min of swimming exercise daily. The erythrocyte glutathione levels of groups 2 and 4 were significantly higher than those of groups 1 and 3 (p<0.01). The plasma malondialdehyde level of group 1 was significantly higher than all other groups. The glutathione levels in liver, kidney, striated muscle, and testes of group 2 were higher than in the other groups (p<0.01) and higher in kidney and striated muscle of group 3 than in groups 1 and 4 (p<0.01). The tissue malondialdehyde levels of striated muscle, liver, kidney, and testes of group 1 were significantly higher than for all other groups (p<0.01). Our findings suggest that both swimming exercise and zinc deficiency result in an increase of lipid peroxidation in tissues and that zinc supplementation prevents these alterations by the activation of the antioxidant system.Öğe Lipid peroxidation in liver tissue of ovariectomized and pinealectomized rats: effect of estradiol and progesterone supplementation(WILEY, 2007) Öztekin, Esma; Tiftik, Ali Muhtar; Baltacı, Abdülkerim Kasım; Moğulkoç, RasimThe present study aimed to determine the effect of estradiol-progesterone supplementation and pinealectomy on lipid peroxidation of liver tissue in ovariectomized rats. The study was carried out on 36 adult Sprague-Dawley female rats, which weighed 200-250 g. The rats were divided into 6 groups: Group 1: Sham Ovariectomy (Sham-Ovx), Group 2: Ovariectomy (Ovx), Group 3: Ovx + Estradiol-Progesterone supplementation (Ovx + H), Group 4: Sham Pinealectomy and Ovx (Sham Pnx -Ovx), Group 5: Ovx -Pnx, Group 6: Ovx -Pnx + H. Malondialdehyde (MDA), reduced form of glutathione (GSH) and glutathione peroxidase (GSH-Px) levels were determined in liver tissue of rats. The highest MDA levels and the lowest GSH-Px levels were determined in the ovariectomized-pinealectomized group, whereas the lowest MDA was in the Sham-Ovx group, and the highest GSH-Px levels were found in the Sham-Ovx and Ovx + Hormone supplemented group. Furthermore, the highest GSH levels were in group 1 and lowest levels were in group 5. The findings of this study demonstrate that ovariectomy led to lipid peroxidation in liver tissues of rats. Pinealectomy in addition to ovariectomy, increases lipid peroxidation, but, estradiol and progesterone supplementations to the ovariectomized-pinealectomized rats protect against lipid peroxidation to a significant extent. Copyright (C) 2006 John Wiley & Sons, Ltd.Öğe Lipid peroxidation in ovariectomized and pinealectomized rats: the effects of estradiol and progesterone supplementation(WILEY, 2007) Öztekin, Esma; Baltacı, Abdülkerim Kasım; Tiftik, Ali Muhtar; Moğulkoç, RasimIn the present study, we investigated the effect of estradiol and progesterone supplementation on oxidant and antioxidant parameters of renal tissue in ovariectomized and pinealectomized rats. The study was carried out on 36 adult, Spraque-Dawley strain female rats, 6 months of age and weighing 200-250g. The rats were divided into six groups, each group included six rats: Group 1: Sham-ovariectomized (Sham-Ovx) Group 2: Ovariectomized (Ovx) Group 3: Ovx and estradiol (E) and progesterone (P) supplemented (Ovx+E-P) Group 4: Ovariectomized and sham pinealectomy (Ovx+sham Pnx) Group 5: Ovariectomized+Pinealectomized (Ovx+Pnx) Group 6: Ovariectomized+Pinealectomized+Hormone Supplemented group (Ovx+Pnx+E-P) The levels of malondialdehyde (NIDA), reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) were analysed in renal tissues of rats. The highest and the lowest levels of MDA were determined in Groups 5 and 1 respectively (p < 0.001). However, GSH and GSH-Px levels demonstrated statistically important decreases in groups 2, 4, 5 (p < 0.001). The findings of this study demonstrate that ovariectomy leads to oxidative damage in renal tissue. Pinealectomy in addition to ovariectomy greatly increases the oxidative damage. However, female sex hormones supplementations to the Ovx and/or Ovx+Pnx rats protected against lipid peroxidation by activating the antioxidant system. Copyright (c) 2006 John Wiley & Sons, Ltd.Öğe Pinealectomy increases oxidant damage in kidney and testis caused by hyperthyroidism in rats(WILEY, 2006) Moğulkoç, Rasim; Baltacı, Abdülkerim Kasım; Aydın, Leyla; Öztekin, Esma; Tuncer, IşıkThyroid hormones regulate energy metabolism and act on mitochondria which are an important source of free radicals in the cell. The pineal gland activates antioxidant systems via melatonin secretion and thus has a protective function in body tissues. The present study was conducted to determine the oxidative damage caused by hyperthyroidism in kidney and testis tissues of pinealectomized rats. Experimental animals were allocated to three groups: 1, control group; 2, sham pinealectomy-hyperthyroidic group; and 3, pinealectomy-hyperthyroidic group. Hyperthyroidism was induced by A 3-week intraperitoneal administration of thyroxin after sham pinealectomy or pinealectomy. Malondialdehyde (MDA) and glutathione (GSH) levels were determined in kidney and testis tissues. MDA levels of the kidney and testis tissue in the pinealectomy and hyperthyroidic groups were significantly higher than those in the sham pinealectomy-hyper-thyroidic group and the control group (p < 0.001). GSH levels of both kidney and testis tissues were significantly higher in the sham-pinealectomy-hyperthyroidic group when compared to the other two groups (p < 0.001). This increase in GSH levels was more evident in the pinealectomy-hyperthyroidic group than in the control group (p < 0.001). The results of our study demonstrate that MDA and GSH levels in kidney and testis tissues increased due to hyperthyroidism and that pinealectomy made the increase in MDA levels more apparent, while decreasing GSH levels. Copyright (c) 2005 John Wiley & Sons, Ltd.Öğe Tıkayıcı Koroner Arter Hastalarında Serum Igf-i (Insülin Benzeri Büyüme Faktörü-i) Düzeylerinin Glukoz, Insülin, C-peptid, Laktat, Acth Düzeyleri ile Ilişkileri(2000) Öztekin, Esma; Korkut, Bayram; Tiftik, Ali Muhtar; Gürbilek, Mehmet; Özeren, AliAMAÇ: Sunulan araştırmada, serum IGF-I düzeyleri ile glukoz, insülin, C-Peptid, laktat, ACTH ve yaş , BKO (Bel-Kalça Oranı), VKİ (Vücut Kitle İndeksi), BÖ (Bel Ölçüsü) arasındaki ilişkilerin tespit edilmesi ve de bu parametrelerin tıkayıcı arter koroner hastalıklarında ki düzeylerinin ölçülmesi amaçlanmıştır. GEREÇ VE YÖNTEM: Çalışmada; "Kontrol Grubu " olarak efor testi negatif olan 12 vaka. (7 erkek, 5 kadın) ve "Çalışma Grubu " olarak efor testi pozitif, tıkayıcı koroner stenozu %50 olan 12 vaka (7 erkek, 5 kadın) kullanıldı. BULGULAR: IGF-I düzeyleri, total çalışma grubunda (121.913.7 ng/mL) kontrol grubuna göre (179.913.9 ng/mL) önemli (p0.0072) oranda düşük olarak saptandı.Erkek kontrol grubunda 185.521 ng/mL olarak ölçülen IGF-I düzeyleri, çalışma grubunda 124.516 ng/mL dir (p0.045). Kadın çalışma grubunda IGF-I düzeyi kontrol grubuna göre düşük olmasına rağmen, bu fark istatiksel açıdan önemsiz bulundu. IGF-I düzeylerinin kadın kontrol grubunda yaş, BKO ile çalışma kadın ve total gruplarında glukoz ile negatif ilişkileri p0.05 düzeyinde önemlidir. Kadın çalışma grubunda insülinin BKO ve BÖ ile korelasyonları istatistiksel açıdan önemsiz olmasına rağmen, C-Peptidin BKO ve BÖ ile korelasyonları sırasıyla p0.05 ve p0.01 düzeyinde önemlidir. SONUÇ: Çalışma gruplarında serum IGF-I düzeylerinin kontrol gruplarından önemli oranda düşük olması KAH (koroner arter hastalıkları) ile ilişkiler yönünden önemli bir sonuç olduğu ve IGF-I'e ilaveten BP'lerinin (Bağlayıcı Protein) ve reseptörlerinin de inceleneceği daha kapsamlı araştırmaların faydalı olacağı kanaatine varıldı.Öğe Tıkayıcı Koroner Arter Hastalarında Serum IGF-I Düzeyi ve Bazı Parametrelerle Ilişkisi(2000) Öztekin, Esma; Korkut, Bayram; Gürbilek, Mehmet; Özeren, Ali; Tiftik, Ali MuhtarAmaç: Tıkayıcı koroner hastalarında serum IGF-I ile total protein, albumin, ürik asit, trigliserit, total kolesterol, HDL-Kolesterol, yaş, VKİ (vücut kütle indeksi), bel-kalça oranı (BKO) ve bel ölçüsü (BÖ) arasındaki ilişkiyi belirlemek. Yöntem: Çalışmada efor testi negatif olan 12 vaka (7 erkek, 5 kadın) kontrol, efor testi pozitif ve tıkayıcı koroner stenozu % 50 olan 12 vaka (7 erkek, 5 kadın) ise deneme grubu olarak alındı. Bulgular: Deneme grubunda IGF-I düzeyi (12247.43 ng/ml) kontrol grubuna göre (18048.15 ng/ml) düşüktü. Erkeklerde IGF-I düzeyi deneme grubunda (124.543.07 ng/ml) kontrol grubundan (185.556.68 ng/ml) düşük iken; kadınlarda IGF-I düzeyi yönünden deneme (118.158.09 ng/ml) ve kontrol (185.556.68 ng/ml) grupları arasındaki fark önemsizdi. Deneme grubunda IGF-I ile HDL-kolesterol arasında pozitif korelasyon gözlendi. Ürik asit, trigliserit, total kolesterol ve HDL-kolesterol düzeyleri yönünden deneme ve kontrol grupları arasında önemli fark yoktu. Albumin düzeyleri, total deneme grubunda ve erkeklerde kontrol grubuna göre düşük bulundu. Sonuç: KAH ile ilişkiler yönünden ve GH’nun yanında IGF-I’in de lipid düzeylerinin regülasyonlarında yer alabileceğinin belirtisi olabilmesi açısından önemli bir sonuç olduğu kanaatine varıldı.Öğe Zinc Prevention of Electromagnetically Induced Damage to Rat Testicle and Kidney Tissues(HUMANA PRESS INC, 2003) Öztürk, Ahmet; Baltacı, Abdülkerim Kasım; Moğulkoç, Rasim; Öztekin, EsmaThe aim of this study was to investigate the extent of lipid peroxidation when zinc is administered to rats periodically exposed to a 50-Hz electromagnetic field for 5 min at a time over a period of 6 mo. Twenty-four Sprague-Dawley adult male rats were subdivided in groups of eight animals each. Group 1 served as untreated controls, group 2 was exposed to an electromagnetic field but received no additional treatment, and group 3 was exposed to electromagnetic radiation and treated with 3-mg/kg daily intraperitoneal injections of zinc sulfate. The erythrocyte glutathione activity (GSH) and the plasma, testicle, and kidney tissue levels of zinc (Zn) and of malondialdehyde (MDA) were measured in all of the animals. The plasma and testicle MDA levels in group 2 were higher than those in groups 1 and 3, with group 3 values significantly higher than those in group 1 (p < 0.001). The kidney MDA levels in group 2 were higher than in groups 1 and 3 (p < 0.001). The erythrocyte GSH level was lower in group 2 than in groups I and 3, with group 1 significantly lower than group 3 (p < 0.001). In testicle and kidney tissues, the GSH levels in group 1 were lower than for groups 2 and 3, with group 2 significantly lower than group 3 (p < 0.001) The plasma zinc levels were highest in group 3, followed by group 1 and group 2, which showed the lowest value (p < 0.001). These results indicate that testicle and kidney tissue damage caused by periodic exposure to an electromagnetic field are ameliorated or prevented by zinc supplementation.
