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Öğe Adma Levels in Acute and Stable Chronic Obstructive Pulmonary Disease(Blackwell Publishing, 2006) Erdem, S.; Abuşoğlu, Sedat; Ünlü, Ali; Kanat, Fikret[Abstract not Available]Öğe Alterations of thyroid hormone levels in cadmium exposure -- 2(WILEY-BLACKWELL, 2013) Tutkun, E.; Yılmaz, H.; Abuşoğlu, Sedat; Yılmaz, F. M.; Gündüzöz, M.; Bal, Ceylan D.; Ünlü, Ali[Abstract not Available]Öğe Assessment of serum ischemia-modified albumin, prolidase and thiol-disulphide levels in subjects with breast cancer.(UNIV PRESS, 2019) Abuşoğlu, Sedat; Eryavuz, Duygu; Bal, Ceylan; Nural, Cemil; Özcan, Erel; Yıldırımel, Mehmet; Çelik, Saadet; Ünlü, AliBackground: Oxidative damage is of great importance for patients with breast cancer. Thus, studies were performed to identify the relationship between breast cancer and oxidative stress biomarkers. Objectives: In this study, our aim was to find out the oxidative and antioxidant status, serum thiol-disulphide levels in subjects with breast cancer. Methods: This study was conducted between March and June 2018 with 82 control subjects (aged between 3267 years) and 127 breast cancer patients (aged between 27-66 years) (p= 0.058) in Selcuk University Faculty of Medicine, Konya, Turkey. Serum myeloperoxidase (MPO), catalase, prolidase were analyzed with kinetic spectrophotometric and thiol-disulphide, ischemia-modified albumin (IMA), ceruloplasmin were detected by colorimetric methods. Results: Serum levels of catalase [199.3 (16.4-489.9) vs 81.6 (18.2-322.9) (kU/L)], MPO [124 +/- 28 vs 101 +/- 31 U/L], disulphide [25 (11-61) vs 18 (2-41) mu mol/L], IMA [0.66 (0.31-3.30) vs 0.62 (0.19-1.31) absorbance unit (ABSU)] and prolidase levels [2217 +/- 538 vs 1456 +/- 401 U/L] were higher in patients than control subjects (For all p< 0.001 except for IMA p=0.031). Native thiol [342 +/- 60 vs 391 +/- 52 mu mol/L] and total thiol levels [396 +/- 56 vs 430 +/- 52 mu mol/L] were lower in patients compared with the control group (For all p< 0.001). Conclusions: Levels of serum thiol/disulphide and prolidase might be reliable indicators for determining oxidative status in certain patient populations.Öğe Association Between Exaggerated Blood Pressure Response to Exercise and Serum Asymmetric Dimethylarginine Levels(Japanese Circulation Soc, 2010) Kayrak, Mehmet; Bacaksız, Ahmet; Vatankulu, Mehmet Akif; Ayhan, Selim S.; Taner, Alparslan; Ünlü, Ali; Yazıcı, Mehmet; Ülgen, Mehmet S.Background: The exaggerated blood pressure response to exercise (EBPR) is an independent predictor of hypertension. Asymmetric dimethylarginine (ADMA) is an endogenous nitric oxide inhibitor and higher plasma levels of ADMA are related to increased cardiovascular risk. The aim of this study is to identify the relationship between ADMA and EBPR. Methods and Results: A total of 66 patients (36 with EBPR and 30 as controls) were enrolled in the study. EBPR is defined as blood pressure (BP) measurements >= 200/100 mmHg during the treadmill test. All the subjects underwent 24-h ambulatory BP monitoring. L-arginine and ADMA levels were measured using a high performance lipid chromatography technique. The serum ADMA levels were increased in the EBPR group compared to the healthy controls (4.0 +/- 1.4 vs 2.6 +/- 1.1 mu mol/L respectively, P=0.001), but L-arginine levels were similar in the 2 groups (P=0.19). The serum ADMA levels were detected as an independent predictor of EBPR (odds ratio 2.28; 95% confidence interval 1.22-4.24; P=0.002). Conclusions: Serum ADMA levels might play a role in EBPR to exercise.Öğe Astımlı çocuklarda serum periostin ve soluk havasında nitrik oksit düzeyi(2018) Dikener, Ahmet Hakan; Özdemir, Hülya; Ceylan, Ayça; Ünlü, Ali; Artaç, HasibeAmaç: Periostin, havayolu epitelinden ve akciğer fibroblastlarından, IL-4 ve IL-13 aracılığı ile salgılanan ekstrasellüler matriks proteinidir. Eozinofilik havayolu inflamasyonun göstergesi olduğu kabul edilmektedir. Çalışmada, astımlı çocuk hastalarda serum periostin düzeyinin değerlendirilmesi ve soluk havasında nitrik oksit (FeNO) düzeyleri ile korelasyonunun belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmaya 7-17 yaş arası, 60 intermittan ve hafif persistan astımlı (29 allerjik, 31 allerjik olmayan) ve 30 sağlıklı kontrol, toplam 90 çocuk (45 kız, 45 erkek) dahil edildi. Hastaların demografik verileri, eozinofil yüzdesi, solunum fonksiyon testi, deri prik testi ve spesifik IgE düzeyleri kaydedildi. FeNO ve serum periostin düzeyi ölçüldü. Bulgular: Çalışmaya alınan tüm bireylerde serum periostin değerlerinin yaşla anlamlı korelasyonu olduğu ve yaşla birlikte azaldığı görüldü (6-12 yaş grubunda 59.0812.79 ng/dl, 13-18 yaş grubunda 46.1811.64 ng/dl, p0.001). Serum periostin düzeyi allerjik astımlı hastalarda 52.1013.93 ng/dl, allerjik olmayanlar da 54.6113.05 ng/dl olup anlamlı fark saptanmadı. FeNO değerleri hasta ve sağlıklı kontrol grubu ile karşılaştırıldığında farklı değildi (15.5 ppb ve 14.0 ppb, p0.486). Hasta grubunda sadece allerjik hastaların FeNO ve periostin değerleri arasındaki korelasyon değerlendirildiğinde istatistiksel olarak anlamlılık saptanmadı (r0.327, p0.077). Ayrıca, serum periostin düzeyi sadece polen duyarlılığı olan grupla karşılaştırıldığında polisensitize olan astımlı çocuklarda daha yüksekti ancak fark istatistiksel olarak anlamlı değildi (58.215.1 ve 44.658.3, p0.065). Sonuç: Bu çalışma ile yeni biyobelirteç olan serum periostin düzeyi çocukluk çağındaki astımlı hastalarda değerlendirildi ve yaşla anlamlı korelasyonunun olduğu gösterildi. İntermittan ve hafif persistan astımlı hastalarda serum periostin düzeyinde anlamlı değişiklik saptanmadı. Çocukluk çağında orta ve ağır persistan astımda, serum periostin düzeyinin değerlendirilmesi için ayrıca çalışmalara ihtiyaç vardır.Öğe Behçet hastalarında asimetrik dimetilarginin düzeyleri(2014) Öztürk, Bahadır; Vatansev, Hüsamettin; Taner, Alpaslan; Yılmaz, Sema; Dağlı, Mehmet; Ünlü, AliAmaç: Behçet Hastalığı (BH), etiyolojisi tam olarak anlaşılmamış, kronik, inflamatuvar multisistemik bir vaskülittir. Nitrik Oksit (NO), endotel hücrelerinde Nitrik Oksit Sentaz (NOS) enzimi tarafından sentezlenen, vasküler sistem için önemi olan bir moleküldür. Asimetrik dimetilarjinin (ADMA) NOS'ın endojen inhibitörüdür. Bu çalışmada BH'larında ADMA düzeyleri ve ataklarla ilgisinin araştırılması amaçlandı.Metod: 34 Behçet hastası ve 34 sağlıklı kontrol çalışmaya dâhil edildi. Serum ADMA, arjinin ve sitrülin düzeyleri HPLC yöntemi ile saptandı. ESR ve CRP düzeyleri rutin yöntemlerle analiz edildi.Bulgular: Literatür bilgilerine ters olarak Behçet hastalarında ADMA düzeyleri kontrol grubuna göre düşük bulundu. Bunun aksine Arjinin ve Sitrülin düzeyleri Behçet hastalarında anlamlı derecede yüksek bulundu. Hasta grubunda Arjinin/ADMA oranları yüksek bulundu. ADMA ve CRP düzeyleri arasında ters bir korelasyon vardı. Arjinin değerleri de sitrülin ve CRP düzeyleri ile koreleydi. Sonuç: BH da genç yaşta atakların sayısının fazla olmasının sebeblerinden birisi düşük ADMA düzeyleri olabilir. Bizim çalışmamız hasta grubundaki düşük ADMA düzeylerine, yaşlanma ile BH alevlenmelerini ve NO aracılıklı inflamasyonu azaltması nedeniyle muhtemel önleyici bir rol yüklemektedirÖğe Changes in Serum Levels of ADMA, SDMA and L-NMMA with Helicobacter Pylori Eradication(AVES, 2017) Baldane, Süleyman; Korkmaz, Hüseyin; İpekci, Süleyman Hilmi; Sözen, Mehmet; Abuşoğlu, Sedat; Akar, Tarık; Ünlü, AliObjective: Increased asymmetric dimethylarginine (ADMA) levels are associated with reduced nitric oxide (NO) levels in many systems, particularly the cardiovascular system, and cause adverse effects. The objective of this study is to evaluate the effect of eradication therapy in patients infected with Helicobacter pylori (H. pylori) on the serum level of ADMA and other metabolic products of methylarginine. Methods: Patients who were found positive both in urea breath tests and stool antigen tests were considered to have H. pylori infection. These patients received eradication therapy for 14 days (twice daily pantoprazole 40 mg, twice daily amoxicillin 1000 mg, and twice daily clarithromycin 500 mg). Blood samples were taken to measure serum ADMA, symmetric dimethylarginine (SDMA), and N-monomethyl-Larginine (L-NMMA) levels before eradication therapy and 3 months after the therapy for patients for whom eradication was achieved. Results: A total of 23 of the 45 patients included in the study were female, whereas 22 were male. The mean age of the patients was 32.4 +/- 8 years. Significant reductions in the serum ADMA, SDMA, and L-NMMA levels of the patients were observed post-eradication therapy versus pre-eradication therapy. Conclusion: This study demonstrated significant reductions in serum ADMA, SDMA, and L-NMMA levels with H. pylori eradication. Further extensive long-term studies are needed to evaluate the positive effects that reduced serum ADMA, SDMA, and L-NMMA levels after H. pylori eradication can have on all systems, particularly the cardiovascular system.Öğe Comparison of immunoassay and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods in the measurement of serum androstenedione levels(WILEY-BLACKWELL, 2016) Yücel, Kamile; Abuşoğlu, Sedat; Ünlü, Ali[Abstract not Available]Öğe Comparison of Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry Methods in the Measurement of Serum Androstenedione Levels(CLIN LAB PUBL, 2018) Yücel, Kamile; Abuşoğlu, Sedat; Ünlü, AliBackground: Recent reports have described inherent problems with androgen immunoassays compared with mass spectrometry analyses. In this study, a new liquid chromatography- tandem mass spectrometry (LC-MS/MS) method was developed according to CLSI rules. The developed method was compared with two immunoassay methods, the enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Methods: After liquid-liquid extraction, a Shimadzu Prominence LC unit coupled to an ABSCIEX API 3200 mass spectrometer with atmospheric pressure chemical ionization was used to quantify serum androstenedione levels. Serum androstenedione results taken from tandem mass spectrometry were compared with the immunoassays. Results: The androstenedione assay was linear up to 50 ng/mL. Lower limit of quantitation and lower limit of detection were 0.195 ng/mL and 0.097 ng/mL, respectively. This method was not affected by matrix effect and other steroid hormone interferences. In this study, the obtained recovery was 69 - 99%, carryover value was determined as 0.026 ng/mL. According to the results of an interference study, androstenedione bias % did not exceed the limit of allowable bias % and 88.7% recovery was acquired for androstenedione. In the LC-MS/MS and ELISA comparison study, the slope value was found as 18.412, intercept -22.87, and r(2) value as 0.1033. In the LC-MS/MS and RIA comparison study, slope value was found as 1.085, intercept 0.4541, and r(2) value as 0.3712. In the RIA and ELISA comparison study, slope value was found as 9.57, intercept -15.5, and r(2) value as 0.19. Conclusions: The LC-MS/MS provides agreement with the results of radioimmunoassay but not with ELISA. This method offers better selectivity compared to immunoassay systems.Öğe Comparison of the Results of Sodium, Potassium, and Chlorine Measured Through Ion Selective Electrode (ISE) Method in Different Devices(Selçuk Üniversitesi, 2022) Akdağ, Turan; Kader, Saadet; Dost, Hüseyin; Ünlü, AliObjective: The measurements of electrolytes are an indispensable part of the routine tests performed in clinical biochemistry laboratories. In the present study, we aimed to evaluate Na, K, and Cl electrolytes with the ISE method in the Mindray BS-800M and Beckman Coulter AU5800 devices. Material and methods: The serum samples were obtained from the healthy volunteers admitted to the Karapınar State Hospital for control purposes between January and April 2021. The sample probe was conducted using the same indirect method in the Mindray BS-800M and Beckman Coulter AU5800 devices, which is an individual sample probe for the ISE method. Results: While the comparative measurements were performed on different devices, the correlations between the electrolytes were found as follows: Na [r=0.964, 95% CI (0.52-0.90)], K [r=0.995, 95% CI (0.09-0.12)], and Cl [r=0.972, 95% CI (0.69-1.12)]. Moreover, a correlation was also found between these results. Conclusion: A consistency between the measured Na, K, and Cl electrolytes was determined on the different analyzers. Also, a positive correlation was determined between those electrolytes. We consider that further and comprehensive studies are needed to elucidate these results.Öğe Determinaton of asymmetric dimethylarginine in body fluids by ABSCIEX API 3200 liquid-chromatography mass spectrometry(WILEY-BLACKWELL, 2013) Ünlü, Ali; Abuşoğlu, Sedat; Akyürek, F.[Abstract not Available]Öğe The early effect of coronary surgery on serum Nt-Pro Bnp levels(WILEY-BLACKWELL, 2015) Akyürek, F.; Ünlü, Ali; Abuşoğlu, Sedat; Oc, M. A. H.[Abstract not Available]Öğe Effect of analysis delay on vitamin D measurement by liquid-chromatography mass spectrometry(WILEY-BLACKWELL, 2015) Ünlü, Ali; Abuşoğlu, Sedat; Akyürek, F.[Abstract not Available]Öğe The effect of vitamin D on MCF-7 breast cancer cell metabolism(COMENIUS UNIV, 2017) Saraçlıgil, B.; Öztürk, B.; Ünlü, Ali; Abuşoğlu, Sedat; Tekin, G.The role of vitamin D in calcium absorption and bone health is known. The studies revealed that vitamin D modulates breast cancer cell growth and it is also associated with a reduced breast cancer risk. The primary objective of this study was to highlight the metabolic effect of Vitamin D on MCF-7 breast cancer cell line. For that purpose, we checked the apoptosis, energy, amino-acid and acylcarnitine levels in cancer cells, that the study propose, that la, 25(OH)(2)D-3 could inhibit cell growth in a dose and time dependent manner. IC50 dose was calculated as 145 nM for vitamin D. We observed the apoptosis level in vitamin D groups, which were 18, 28 and 38.5 % at 24, 48 and 72 hours, respectively. During metabolic screening analysis, it was observed that glutamine, methionine and glutamic acid levels were treated more by Vitamin D groups in cell line and also, that acylcarnitine level was increased in 24 and 48 hour groups when compared to the control, but decreased in 72 hours. Further studies are needed to analyze the role of amino acids and acylcarnitines for early apoptosis and cancer metabolism (Tab. 2, Fig. 4, Ref. 24). Text in PDF www.elis.sk.Öğe The effects of prophylactic zinc and melatonin application on experimental spinal cord ischemia-reperfusion injury in rabbits: experimental study(NATURE PUBLISHING GROUP, 2007) Kalkan, E.; Çiçek, O.; Ünlü, Ali; Abuşoğlu, Sedat; Kalkan, S. S.; Avunduk, M. C.; Baysefer, A.Study design: Experimental study. Objectives: To determine the neuroprotective effects of zinc and melatonin on spinal cord ischemia-reperfusion (I/R) injuries of rabbits. Setting: The Experimental Research Centre of Selcuk University, Konya, Turkey. Methods: Twenty-four male rabbits underwent spinal cord ischemia by clamping the thoraco-abdominal aorta for 20 min. Twenty-minutes before the aortic clamping, animals received zinc, melatonin or a combination of both. Neurological examination of the animals was performed three times during reperfusion period. The animals were killed 24 h after reperfusion. Spinal cord samples were taken for biochemical and histopathological evaluation. Results: Pre-treated animals with zinc, melatonin or combination displayed better neurological outcomes than the I/R group (P < 0.05). Zinc, melatonin and combined treatment prevented spinal cord injury by reducing apoptosis rate (P < 0.05) and preserving intact ganglion cell numbers (P < 0.05). Zinc pre-treatment protected spinal cord by preventing malondialdehyde (MDA) formation (P = 0.002), increasing glutathione peroxidase (GPx) activity(P = 0.002) and decreasing xanthine oxidase enzyme activity (P = 0.026) at molecular level. Melatonin treatment also resulted with MDA formation (P = 0.002), increased GPx activity (P = 0.002) and decreased xanthine oxidase activity(P = 0.026). Conclusion: The results of the study showed that prophylactic zinc and melatonin use in spinal cord I/R not only suppressed lipid peroxidation by activating antioxidant systems but also had significant neuroprotective effects by specifically improving the neurological and histopathological situation.Öğe EFFECTS OF VITAMIN D REPLACEMENT ON THE DISEASE ACTIVITY AND INFLAMMATORY PARAMETERS SUCH AS IL-1, IL-6, TNF-alpha, ASYMMETRIC DIMETHYLARGININE (ADMA) AND ARGININE/ADMA RATIO IN ANKYLOSING SPONDYLITIS(BMJ PUBLISHING GROUP, 2017) Esmen, S. Ergulu; Yılmaz, S.; Kebapcılar, Levent; Ünlü, Ali; İpekci, S. H.; Abuşoğlu, Sedat[Abstract not Available]Öğe Elevated Urinary Methylmalonic Acid/creatinine ratio and Serum Sterol levels in Patients with Acute Ischemic Stroke(UNIV PRESS, 2018) Sivrikaya, Abdullah; Öztürk, Şerefnur; Ekmekci, Hakan; Sağlam, Aslıhan; Abuşoğlu, Sedat; Ünlü, AliIntroduction: Sitosterolemia, defined as phytosterolemia, is a rare autosomal recessive disease characterized by elevated blood sterol levels. Our aim was to investigate serum plant sterols, methylmalonic acid, vitamin B12, oxidized-LDL and homocysteine levels in ischemic and hemorrhagic stroke patients and healthy subjects. Material and Methods: 50 healthy subjects (without a family history of coronary artery disease) and 89 patients hospitalized in the Selcuk University neurology clinic or intensive care unit with a diagnosis of stroke were included in this study. Serum plant sterols, homocysteine and methylmalonic acid, oxidized-LDL, total cholesterol, triglycerides, HDL-Cholesterol and vitamin B12 levels were analyzed by gas chromatography-mass spectrometry, liquid-chromatography tandem mass spectrometry, commercially available ELISA kit, spectrophotometry and chemiluminescence methods, respectively. Results: Urinary methylmalonic acid/creatinine ratio (p< 0.05), serum beta-sitosterol levels and beta-sitosterol/cholesterol ratio were significantly higher (p < 0.01) in patients compared to the control group. There was a significant positive correlation between the serum OxLDL-methylmalonic acid, serum homocysteine-urinary methylmalonic acid/creatinine ratio, serum methylmalonic acid-Urinary methylmalonic acid (p< 0.05), serum homocysteine-urinary methylmalonic acid, urinary methylmalonic acid-methylmalonic acid/creatinine ratio, serum methylmalonic acid-methylmalonic acid/creatinine ratio, serum beta-sitosterol-beta-sitosterol/cholesterol, total cholesterol-HDL, total cholesterol-LDL (p < 0.01) levels and negative correlation between vitamin B12-serum methylmalonic acid (p< 0.05), cholesterol-stigmasterol/cholesterol, LDL-stigmasterol/cholesterol (p < 0.01) levels in the patient group. Conclusion: Our findings presented that the serum sitosterol levels were significantly higher in stroke patients compared to controls.Öğe Evaluation of bone mineral density (BMD) and indicators of bone turnover in patients with hemophilia(ASSOC BASIC MEDICAL SCI FEDERATION BOSNIA & HERZEGOVINA SARAJEVO, 2018) Dağlı, Mehmet; Kutlucan, Ali; Abuşoğlu, Sedat; Baştürk, Abdulkadir; Sözen, Mehmet; Kutlucan, Leyla; Ünlü, Ali; Yılmaz, FariseA decrease in bone mass is observed in hemophilic patients. The aim of this study was to evaluate bone mineral density (BMD), parathyroid hormone (PTH), 25-hydroxy vitamin D (vitamin D), and a bone formation and resorption marker, procollagen type I N-terminal propeptide (PINP) and urinary N-terminal telopeptide (uNTX) respectively, in hemophilic patients and healthy controls. Laboratory parameters related to the pathogenesis of bone loss such as neutrophil- lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) were also evaluated. Thirty-five men over 18 years of age, with severe hemophilia (A and B) and receiving secondary prophylaxis, were included in the study. The same number of age-, sex-, and ethnicity-matched healthy controls were evaluated. Anthropometric, biochemical, and hormonal parameters were determined in both groups. No significant difference in anthropometric parameters was found between the two groups. The BMD was low in 34% of hemophilic patients. Vitamin D, calcium, and free testosterone levels were significantly lower (p < 0.001, p = 0.011, p < 0.001, respectively), while PTH, PINP, and activated partial thromboplastin time (aPTT) levels were significantly higher (p < 0.014, p = 0.043, p < 0.001, respectively), in hemophilic patients compared to controls. There was no significant difference between the two groups in NLR, PLR, phosphorus, thyroid-stimulating hormone, and uNTX level. The reduction of bone mass in hemophilic patients may be evaluated using the markers of bone formation and resorption, enabling early detection and timely treatment.Öğe EVALUATION OF BONE MINERAL DENSITY AND PARAMETERS RELATED TO BONE METABOLISM IN HEMOPHILIC PATIENTS(PERGAMON-ELSEVIER SCIENCE LTD, 2017) Dağlı, M.; Kutlucan, A.; Abuşoğlu, Sedat; Baştürk, A.; Sözen, Mehmet; Kutlucan, L.; Ünlü, Ali[Abstract not Available]Öğe Evaluation of fractalkine (FKN) and secreted frizzled-related protein 4 (SFRP-4) serum levels in patients with prediabetes and type 2 diabetes(COMENIUS UNIV, 2018) Baldane, S.; İpekci, S. H.; Ekin, A.; Abuşoğlu, Sedat; Ünlü, Ali; Kebapcılar, LeventOBJECTIVE: The objective of this study is to compare serum levels of FKN and SFRP-4 in patients with normal glucose tolerance (NGT), impaired glucose tolerance (IGT), and type 2 diabetes mellitus (T2DM). METHODS: A total of 152 patients presented to the endocrinology outpatient clinic of our hospital were included in the study. Eighty-two patients with a history of T2DM were assigned to the T2DM group. IGT (n = 34) and NGT (n = 36) groups included the patients who received oral glucose tolerance test outcomes. RESULTS: Serum FKN levels were significantly higher in the IGT and T2DM groups compared to the NGT group (p < 0.001 and p < 0.001, respectively). Serum SFRP-4 levels were significantly higher in the T2DM group compared to the IGT and NGT groups (p = 0.001 and p = 0.004, respectively). A significant correlation was observed between FKN and fasting glucose levels. SFRP-4 was significantly correlated with fasting glucose, HbA1c, and triglyceride levels. CONCLUSION: To our knowledge, increased FKN levels in patients with IGT were demonstrated for the first time in this study. The results of our study support the opinion that FKN and SFRP-4 may contribute to the pathogenesis of T2DM.
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