The effects of prophylactic zinc and melatonin application on experimental spinal cord ischemia-reperfusion injury in rabbits: experimental study
Küçük Resim Yok
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
NATURE PUBLISHING GROUP
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Study design: Experimental study. Objectives: To determine the neuroprotective effects of zinc and melatonin on spinal cord ischemia-reperfusion (I/R) injuries of rabbits. Setting: The Experimental Research Centre of Selcuk University, Konya, Turkey. Methods: Twenty-four male rabbits underwent spinal cord ischemia by clamping the thoraco-abdominal aorta for 20 min. Twenty-minutes before the aortic clamping, animals received zinc, melatonin or a combination of both. Neurological examination of the animals was performed three times during reperfusion period. The animals were killed 24 h after reperfusion. Spinal cord samples were taken for biochemical and histopathological evaluation. Results: Pre-treated animals with zinc, melatonin or combination displayed better neurological outcomes than the I/R group (P < 0.05). Zinc, melatonin and combined treatment prevented spinal cord injury by reducing apoptosis rate (P < 0.05) and preserving intact ganglion cell numbers (P < 0.05). Zinc pre-treatment protected spinal cord by preventing malondialdehyde (MDA) formation (P = 0.002), increasing glutathione peroxidase (GPx) activity(P = 0.002) and decreasing xanthine oxidase enzyme activity (P = 0.026) at molecular level. Melatonin treatment also resulted with MDA formation (P = 0.002), increased GPx activity (P = 0.002) and decreased xanthine oxidase activity(P = 0.026). Conclusion: The results of the study showed that prophylactic zinc and melatonin use in spinal cord I/R not only suppressed lipid peroxidation by activating antioxidant systems but also had significant neuroprotective effects by specifically improving the neurological and histopathological situation.
Açıklama
Anahtar Kelimeler
ischemia, reperfusion, zinc, melatonin, oxidative damage, rabbit
Kaynak
SPINAL CORD
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
45
Sayı
11
