Yazar "Atalay, Emine" seçeneğine göre listele
Listeleniyor 1 - 14 / 14
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Buğday (kızıltan-91) ve arpa (tokak-157/37) in vitro fidelerinde bor alımının ICP-AES ile tespiti(Selçuk Üniversitesi Fen Bilimleri Enstitüsü, 2003-01-28) Atalay, Emine; Babaoğlu, MehmetBuğday (Triticum durum ev. Kızıltan-91) ve arpa (Hordeum vulgar e ev. Tokak-157/37) in vitro fidelerinde bor alımı ve çeşitli organlarda bor birkiminin dağılımı ICP-AES (Inductivelly Coupled Plasma-Atomic Emission Spectrometry- Varian Vista Model Axiel) kullanılarak araştırılmıştır. Ayrıca farklı bor konsantrasyonlanmn tohumların çimlenme yüzdeleri üzerine etkisi değerlendirilmiştir. Tohumlar; 200 ml'lik cam kavanozlarda, %0.7 agar, %3 sakkaroz ve sırasıyla; 0.0, 6.2, 18.6, 55.8, 111.6 mg/1 H3B03 (0.00, 1.05, 3.25, 9.76, 19.5 mg B/l) içeren 50 mi MS besin ortamında, her kavanozda 5 adet tohum olacak şekilde kültüre alınmıştır. Bor alımı ve birikiminin tespiti için, 20 günlük fidelerin kurutulmuş kök ve gövde kısımları (0.1-1 g) ve fidelerin yetiştiği besin ortamları (5.0-6.0 g) mikrodalgada (CEM-Mars x 5) 10 mi HN03 ile 170 PSI basınçta 200°C'de 40 dak. yakılmış ve numunelerde ICP-AES ile bor analizi yapılmıştır. Kızıltan-91 'in köklerindeki en düşük bor birikimi (2.2 ug B/l) bor konsantrasyonun 0 mg/1 H3BO3 olduğu ortamda kültüre alınanlarda bulunurken, en yüksek bor birikimi (15.1 ug B/l) en yüksek ortam bor konsantrasyonunda (111.6 mg/1 H3BO3) tespit edilmiştir. Benzer şekilde Kızıltan-91'in gövdesi tarafından en yüksek seviyede bor alımı (67.6 ug B/l) bor konsantrasyonunun en yüksek olduğu ortamda (111.6 mg/1 H3BO3) olmuştur. Tokak-157/37 fidelerinde kök ve gövdelerindeki bor birikimi de Kızıltan-91 'e benzemektedir.Öğe Callus Induction and Plant Regeneration of the Endemic Astragalus Nezaketae in Turkey(Univ Catolica De Valparaiso, 2010) Erişen, Semiha; Yorgancılar, Mustafa; Atalay, Emine; Babaoğlu, Mehmet; Duran, AhmetA callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.Öğe Determination of Minerals Extracted From Several Commercial Teas (Camellia Sinensis) to Hot Water (Infusion)(Mary Ann Liebert, Inc, 2006) Gezgin, Sait; Özcan, Mehmet Musa; Atalay, EmineMineral contents of some tea and their infusions drunk in Turkey were established by inductively coupled plasma atomic emission spectrometry. The Al, Ca, K, Mg, Mn, P, and S contents were very high in both infusions and tea (i.e., pieces of the tea plant). The As, Cd, Cr, Li, Ph, and Se contents of infusion and tea were found to be very low. The level of K of all samples is higher than those of other minerals. Generally, mineral contents of tea were found to be higher than those of tea infusions. In addition, the health benefits of teas and knowledge of their mineral contents are of great interest and may be useful for further study of enzyme systems and vital biochemical functions.Öğe Determination of the relationship among Old World Lupin (Lupinus sp.) species using RAPD and ISSR markers(ACADEMIC JOURNALS, 2009) Yorgancilar, Mustafa; Babaoglu, Mehmet; Hakki, Erdogan E.; Atalay, EmineRandomly Amplified Polymorphic DNA ( RAPD) and Inter Simple Sequence Repeat (ISSR) markers were used to determine the genetic relationships among 20 Old World lupin genotypes from 3 lupin species (Lupinus albus, L. angustifolius, L. luteus) using 15 primers in each case. Polymorphism was observed in reactions with 9 out of 15 tested RAPD primers, and 11 out of 15 tested ISSR primers. Relationship was determined on the basis of polymorphic products analysis which was shown in the form of dendrograms by UPGMA method. Both RAPD and ISSR markers worked well to identify distinct grouping among three species and among genotypes within a species. The parallel use of both data set seems to allow precise estimation of cultivars relationship and diminishing mistakes connected with method's technical limitations. Such marker systems may potentially be used in the development of new lupins cultivars.Öğe The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2011) Erisen, Semiha; Atalay, Emine; Yorgancilar, MustafaThidiazuron (TDZ) is a cytokinin-like substance that has often been used for shoot regeneration in recent years. We observed the effect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss. Explants were cultured on Murashige and Skoog (MS) media including TDZ (in concentrations of 0.2, 0.4, or 0.6 mg/L) or in combination with alpha-naphthaleneacetic acid (NAA) (in concentrations of 0.2 or 1.0 mg/L). Significant variations in the frequency of callus formation and the morphogenesis of the callus were obtained depending on the explant type. Petiole explant was determined to be the best for callus formation, but calli from leaf explants were more responsive in terms of shoot formation. MS media including TDZ and NAA showed 100% callus formation, but shoot regeneration was enhanced by TDZ combined with a low concentration of NAA. The highest number of shoots, (15 per explant) was induced from leaf explants on MS medium with 0.4 mg/L TDZ and 0.2 mg/L NAA. Regenerated shoots were rooted in MS medium containing 0.5 mg/L indole-3-butyric acid (IBA). The rooted plantlets were acclimatised and produced normal plants.Öğe EFFECTS OF DIFFERENT TYPES OF MYCORRHIZA ON THE DEVELOPMENT AND THE ELEMENTAL CONTENT OF LUPIN (LUPINUS ALBUS L.)(POLISH SOCIETY MAGNESIUM RESEARCH, 2016) Akay, Aysen; Yorgancilar, Mustafa; Atalay, EmineThe effects of different types of arbuscular mycorrhizal (AM) fungi on the root inoculation and plant elemental content of lupin (Lupinus albus L.) were investigated in the present study. The growth and development of lupin were examined to determine the species of AM fungi that can help to grow lupin with a high protein content and economic value. In this study, which was carried out as a pot experiment under controlled greenhouse conditions, first the pots were inoculated with Glomus geosporum, Glomus mosseae, Glomus caledonium, Glomus etunicatium mycorrhizal spores and then lupin (Lupinus albus) seeds were sown. The plants were watered with pure water during the experiment. The trial was terminated after a 60-day plant gowing period. In the study, inoculation occurred at lupin roots at rates varying between 13.3 and 30.0%. However, there was no statistically significant difference among the types of arbuscular mycorrhizal fungi applied to the plant in the inoculation rate. The examination of the effect of the application of different AM (Glomus geosporum, Glomus mosseae, Glomus caledonium, Glomus etunicatium) spore on the plant development showed that AM inoculation did not have an effect on the lupin development. The effect of AM inoculation on the plant's nutrient content revealed no significant difference in the content of crude protein, P and K, while demonstrating a significant increase in the sulphur and magnesium content versus the control. The plant content of crude protein varied between 185.6 and 226.5 (g kg(-1)), phosphorus 0.61-0.74 (g kg(-1)) and potassium - 9.6-11.1 (g kg(-1)). The concentrations of Zn, Cu, B and Mo in lupin did not show statistically significant modifications caused by the inoculation of different types of AM. However, the plant Mn content showed a decrease due to AM inoculation, whereas a significant increase was observed in the Na content after AM inoculation. AM fungi were observed in plant roots after the inoculation with any of the four different types of mycorrhiza. But no positive effects of mycorrhizal inoculation were not observed on crude protein and the uptake of plant nutrients.Öğe Endemik astragalus trojanus stev’in nodal kültür ile mikroçoğaltımı(2017) Atalay, Emine; Tanur Erkoyuncu, Münüre; Erişen, Semiha; Yorgancılar, MustafaBu araştırmada, tıbbi özelliklere sahip endemik Astragalus trojanus Stev. bitkisinin nodal kültürler ile klonal çoğaltımı amaçlanmıştır. Tohumlar Murashige ve Skoog (MS) besin ortamında çimlendirilmiş ve elde edilen steril fidelerin nodal segmentleri eksplant olarak kullanılmıştır. İlk olarak MS ve Woody Plant Medium (WPM) besin ortamlarının sürgün çoğaltımına etkileri araştırılmıştır. Eksplantlar farklı konsantrasyonlarda (0.2, 0.5, 1.0, 2.0 mg/L) 6-benzil amino purin (BAP) içeren MS ya da WPM besin ortamlarında kültüre alınmıştır. Kültürden 4 hafta sonra en fazla sürgün sayısı (2.80 adet/eksplant) 0.5 mg/L BAP içeren WPM ortamında elde edilmiştir. Çalışmanın devamında nodal segmentler, sitokinin kaynağı ve konsantrasyonlarının sürgün çoğaltımına etkisini belirlemek amacıyla BAP, thidiazuron (TDZ), kinetin (KIN)'in farklı konsantrasyonlarını (0.2, 0.5, 1.0, 2.0 mg/L) içeren WPM temel besin ortamında kültüre alınmıştır. 4 hafta sonunda elde edilen sürgün sayıları bakımından TDZ ve KIN'in benzer etki gösterdiği, en yüksek sürgün sayısının (2.09 adet/eksplant) BAP'dan elde edildiği tespit edilmiştir. Rejenere sürgünler köklenme için 1 veya 3 mg/L indol-3-butirik asit (IBA), naftalen asetik asit (NAA) veya indol-3-asetik asit (IAA) içeren WPM ortamlarına aktarılmıştır. Kültürden 4 hafta sonra en yüksek köklenme yüzdesi (%80) 3 mg/L IBA içeren WPM ortamından elde edilirken, en fazla kök sayısı (4.33 adet/eskplant) 3 mg/L IAA içeren WPM ortamından elde edilmiştir. Köklenen bitkicikler 1:1 oranında torf ve perlit içeren plastik bardaklara aktarılarak dış ortama başarıyla alıştırılmış ve tam bitki gelişimi 15 hafta sonunda tamamlanmıştır. Bu araştırma ile, Astragalus trojanus Stev. endemik türünde etkili bir klonal çoğaltım metodu tanımlanmıştırÖğe Genotypic Variability for Seed Protein in Barley Germplasm(Selçuk Üniversitesi, 2018) Akgün, Necdet; Atalay, EmineBarley accessions collected from South-West part of Central Anatolia, Turkey, were evaluated for seed protein for two seasons. A wide range of variation (6 to 19%) was found in the germplasm studied during both seasons. Maximum accessions exhibited 12.1-15% protein, whereas few accessions produced more than 16% protein. Correlation between two seasons’ data (r=0.417**) was highly significant indicating the influence of environment component. Germplasm were classified on the basis of areas located, altitude and lemma colour in the region referred. Thus, accessions from Eastern areas possessed average higher protein percentage followed by accessions from Southern areas. Classification on the basis of altitude showed that the accessions collected from 900-1100 masl (meters above sea level) had lower protein while those collected from higher levels had higher protein. According to lemma colour, accessions with white lemma are more situated in the lower levels while those collected from higher levels had dark lemma. In this way, the study provides information on important protein sources of barley germplasm.Öğe HIGH FREQUENCY REGENERATION OF CRITICALLY ENDANGERED ENDEMIC PLANT CENTAUREA LYCAONICA(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2017) Atalay, Emine; Erisen, SemihaCentaurea lycaonica is a critically endangered endemic plant species found in Turkey. In this study in vitro culture techniques were evaluated as an ex situ strategy for conservation of this species. Achenes were surface sterilized and cultured either in MS medium alone or along with GA(3) in an attempt to induce germination. It was observed that the overall germination rate was low and that the highest rate (20%) was obtained when hormone-free MS medium was used. For axillary shoot pro-pagation, nodal explants were cultured on MS me-dium supplemented with BAP, Kin or TDZ. The highest shoot number (8.7 shoots per explant) was obtained on MS medium supplemented with 0.5 mg L BAP. Leaf explants were removed from the in vitro axillary shoots that were used as explants and cultured on medium including BAP, Kin, TDZ alone or with NAA. The calli obtained exhibited differences in morphology which appeared to be dependent on type and combination of PGR used. It was found that BAP was the only PGR that was capable of eliciting a positive response with respect to shoot regeneration. The highest shoot number (32.3 shoots per explant) was obtained on MS medium supplemented with 0.5 mg L-1 BAP and 0.2 mg L-1 NAA. Regenerated shoots were transferred into various rooting media. The best rooting rate (90%) was obtained on MS medium supplemented with 2 mg L-I IAA. Rooted shoots were successfully acclimatized and were observed to grow vigorously under ex vitro conditions.Öğe In vitro shoot multiplication of Anagyris foetida L.(CURRENT BIOLOGY LTD, 2011) Erisen, Semiha; Atalay, Emine; Yorgancilar, Mustafa; Oncel, Zeynep[Abstract not Available]Öğe Mechanisms of Boron Tolerance and Accumulation in Plants: a Physiological Comparison of the Extremely Boron-Tolerant Plant Species, Puccinellia Distans, with the Moderately Boron-Tolerant Gypsophila Arrostil(AMER CHEMICAL SOC, 2010) Stiles, Amanda R.; Bautista, David; Atalay, Emine; Babaoğlu, Mehmet; Terry, NormanThe physiological characteristics of the extremely boron (B)tolerant plant species, Puccinellia distans, were compared with those of the moderately tolerant Gypsophila arrostil, two species collected from a B-mining area of Eskisehir, Turkey. Boron was supplied to plants hydroponically at B concentrations ranging from 0.5 to 50 mg B/L for G. arrostil, and from 0.5 to 2000 mg B/L for P. distans. The results show that P. distans has a strikingly greater tolerance to B than G. arrostil. While G. arrostil was unable to survive B supply concentrations greater than 50 mg B/L, P. distans grew at B supply concentrations exceeding 1250 mg B/L. Our research supports the conclusion that from 0.5 to 50 mg B/L, P. distans is better able to restrict the accumulation of B in the whole plant, and the transport of B from root to shoot, than G. arrostil. We propose that P. distans uses several strategies to achieve B tolerance including the ability to restrict the accumulation of B relative to its accumulation of biomass, the ability to restrict the transport of B from root to shoot, and, to a lesser extent, the ability to tolerate high concentrations of B in its shoot and root tissues.Öğe Micropropagation of Stevia rebaudiana Bertoni(CURRENT BIOLOGY LTD, 2011) Atalay, Emine; Erişen, Semiha; Yorgancılar, Mustafa; Tanur, Münüre[Abstract not Available]Öğe Prolific Shoot Regeneration of Astragalus Cariensis Boiss(Springer, 2010) Erişen, Semiha; Yorgancılar, Mustafa; Atalay, Emine; Babaoğlu, MehmetProlific shoot regeneration via organogenesis was induced from leaf and leaf petiole explants of the endemic Astragalus cariensis species on Murashige and Skoog (MS) medium with alpha-naphthaleneacetic acid (NAA) and benzyladenine (BA) within 8 week. The highest number of shoots (23/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. Elongated shoots were successfully rooted in MS medium with 0.5 mg/l indole-3-butyric acid. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.Öğe Türkiye'deki tescilli nohut (Cicer arietinum L.) çeşitlerinin ve bazı nohut genotiplerinin demir uygulamalarına gösterdikleri tepkilerin ve genetik akrabalık derecelerinin belirlenmesi(Selçuk Üniversitesi Fen Bilimleri Enstitüsü, 2009) Atalay, Emine; Babaoğlu, MehmetBu çalışma Türkiye'deki tescilli nohut (Cicer arietinum L..) çeşitlerinin ve bazı nohut genotiplerinin demir uygulamalarına gösterdikleri tepkilerin ve genetik akrabalık derecelerinin belirlenmesi amacıyla yapılmıştır. Araştırmada 20 tescilli çeşit, 6 hat ve 3 köy populasyonu materyal olarak kullanılmıştır. Deneme kontrollü sera şartlarında saksılarda tesadüf parsellerinde faktöriyel deneme desenine göre 4 tekerrürlü kurulmuştur. Denemede demir noksan toprak kontrol olarak kullanılırken, uygulama grubu topraklara 10 mg Fe kg-1 olacak şekilde sequestrin formunda demir uygulaması yapılmıştır. Bitki boyu, ana dal sayısı, kuru ağırlık, bakla sayısı, dolu ve boş bakla sayısı, bitkide tane sayısı ve ağırlığı, 100 tane ağırlığı üzerine Fe uygulamasının etkisi istatistiki anlamda önemli olmamış ancak, genotipik farklılık gözlenmiştir. Elementel analizlerin sonuçlarına göre Fe uygulamasının genotiplerin aktif demir (Fe+2) içeriklerini artırdığı belirlenmiştir. Bitkilerin yaprak, gövde ve tohum kısımlarında toplam Fe (Fe+2 + Fe+3) içerikleri de demir uygulaması ile artış göstermiştir. Bitki kısımlarının Zn ve Cu içeriklerinin Fe uygulamasıyla arttığı, Mn içeriklerinin ise belirgin şekilde azaldığı tespit edilmiştir. Fe uygulamasının genotiplerin klorofil içeriklerinde artışa neden olduğu belirlenmiştir. ISSR ve SSR moleküler markörleri ile yapılan genetik akrabalığı belirleme çalışmalarının sonuçlarına göre kullanılan nohut genotiplerinin yüksek oranda benzerlik gösterdikleri ve genetik tabanın dar olduğu görülmüştür.
