Callus Induction and Plant Regeneration of the Endemic Astragalus Nezaketae in Turkey
Yükleniyor...
Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Univ Catolica De Valparaiso
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.
Açıklama
Anahtar Kelimeler
Astragalus, Callus, Organogenesis, Plant regeneration
Kaynak
Electronic Journal of Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
13
Sayı
6
Künye
Erişen, S., Yorgancılar, M., Atalay, E., Babaoğlu, M., Duran, A., (2010). Callus Induction and Plant Regeneration of the Endemic Astragalus Nezaketae in Turkey. Electronic Journal of Biotechnology, 13(6), 1-7. Doi: 10.2225/vol13-issue6-fulltext-3
