Callus Induction and Plant Regeneration of the Endemic Astragalus Nezaketae in Turkey

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dc.contributor.authorErişen, Semiha
dc.contributor.authorYorgancılar, Mustafa
dc.contributor.authorAtalay, Emine
dc.contributor.authorBabaoğlu, Mehmet
dc.contributor.authorDuran, Ahmet
dc.date.accessioned2020-03-26T17:47:20Z
dc.date.available2020-03-26T17:47:20Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-106O136]en_US
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) Project "Tissue Culture and Cytogenetic Studies in Some Endemic Astragalus L. Species (Leguminosae) for Germplasm Preservation and Agricultural Uses", Number TOVAG-106O136.en_US
dc.identifier.citationErişen, S., Yorgancılar, M., Atalay, E., Babaoğlu, M., Duran, A., (2010). Callus Induction and Plant Regeneration of the Endemic Astragalus Nezaketae in Turkey. Electronic Journal of Biotechnology, 13(6), 1-7. Doi: 10.2225/vol13-issue6-fulltext-3
dc.identifier.doi10.2225/vol13-issue6-fulltext-3en_US
dc.identifier.endpage7
dc.identifier.issn0717-3458en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1
dc.identifier.urihttps://dx.doi.org/10.2225/vol13-issue6-fulltext-3
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24675
dc.identifier.volume13en_US
dc.identifier.wosWOS:000288177100003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorErişen, Semiha
dc.institutionauthorYorgancılar, Mustafa
dc.institutionauthorAtalay, Emine
dc.institutionauthorBabaoğlu, Mehmet
dc.institutionauthorDuran, Ahmet
dc.language.isoenen_US
dc.publisherUniv Catolica De Valparaisoen_US
dc.relation.ispartofElectronic Journal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAstragalusen_US
dc.subjectCallusen_US
dc.subjectOrganogenesisen_US
dc.subjectPlant regenerationen_US
dc.titleCallus Induction and Plant Regeneration of the Endemic Astragalus Nezaketae in Turkeyen_US
dc.typeArticleen_US

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