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Öğe Effect of Lycopene on Caspase-3 Enzyme Activation in Liver of Methanol-Intoxicated Rats: Comparison with Fomepizole(MARY ANN LIEBERT INC, 2010) Kurçer, Mehmet Ali; Kurçer, Zehra; Köksal, Mete; Baba, Füsun; Ocak, Ali Rıza; Aksoy, Nurten; Ateşşahin, Ahmet; Sahna, EnginLycopene is one of the major carotenoids and is found almost exclusively in tomatoes and tomato products. This study was performed to evaluate the effect of lycopene on methanol-induced liver injury and to compare the results with those after fomepizole, which is used in treatment of methanol intoxication. Experiments were carried out with 30 female Wistar rats weighting 180-200 g. Rats were injected with a intraperitoneally dose of 3 g/kg methanol as a 50% solution in isotonic saline once for intoxication. Rats were pretreated with fomepizole (50 mg/kg) and/or lycopene (10 mg/kg) before methanol. After 24 hours all the drug-treated and intoxicated rats were sacrificed under anesthesia. Malondialdehyde (MDA) levels were determined in order to assess lipid peroxidation, and caspase-3 activity was determined by immunostaining of liver tissues to evaluate apoptosis. Methanol administration significantly increased the MDA level and caspase-3 activity in liver. Pretreatment with lycopene and/or fomepizole decreased the MDA levels significantly. Similarly, lycopene and fomepizole decreased methanol-induced caspase-3 activity. The findings of the present study demonstrate that methanol intoxication causes hepatic toxicity in rats and that this is likely a result of reactive oxygen species and apoptosis induction. Lycopene has protective effects against methanol-induced hepatic injury similar to fomepizole. It was demonstrated for the first time that both lycopene and fomepizole prevent methanol-induced hepatic injury by reducing the increase of lipid oxidation and caspase-3 activation.Öğe Effect of Melatonin on Epididymal Sperm Quality After Testicular Ischemia/Reperfusion in Rats(ELSEVIER SCIENCE INC, 2010) Kurçer, Zehra; Hekimoğlu, Aşkın; Aral, Faruk; Baba, Füsun; Sahna, EnginObjective: To determine the effect of melatonin, a pineal secretory product that prevents testicular ischemia/reperfusion (IR) injury through its antioxidative properties, on epididymal sperm quality in a rat testicular IR injury model. Design: Experimental study. Setting: University pharmacology laboratory. Animal(s): Fifty-six 8-week-old male Wistar albino rats. Intervention(s): Left testicular artery and vein occluded for I hour; before the bilateral orchiectomy, the organ was allowed to reperfuse 30 days. Melatonin (10 mg/kg IP) or vehicle (1% ethanol in saline) was administrated for 10 minutes before reperfusion and for 1 hour after reperfusion. Main Outcome Measure(s): After 24 hours of reperfusion, the rats were decapitated, and the testicular tissue samples were obtained for histologic examination. In addition, after 30 days of reperfusion, the epididymal sperm concentration, motility, and abnormal sperm rates were determined in the sperm collected from the epididymis. Result(s): A statistically significant decrease in sperm concentration resulted from IR as well as an increase in sperm abnormalities, but the sperm motility did not change. Melatonin treatment did not prevent the IR-induced reduction in sperm concentration. However, melatonin treatment statistically significantly decreased the sperm abnormalities when compared with the IR injured samples. Conclusion(s): Melatonin may improve sperm morphology for a protective effect in IR-induced testicular injury.Öğe Effects of 3-Aminobenzamide on Unilateral Testicular Ischemia-Reperfusion Injury: What is the Role of PARP Inhibition?(CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS, 2010) Hekimoğlu, Aşkın; Kurçer, Zehra; Aral, Faruk; Baba, Füsun; Ateşşahin, Ahmet; Sakin, FatihOn a examine´ les effets the´rapeutiques de l’inhibition de la poly(ade´nosine diphosphate-ribose) polyme´rase par le 3-aminobenzamide (3-AB) dans une le´sion d’ische´mie-reperfusion (I/R) des testicules en utilisant une analyse de sperme. On a effectue´ des examens histopathologiques et biochimiques pour mesurer les activite´s de la superoxyde dismutase (SOD), de la catalase (CAT), de la glutathion peroxydase (GSH-Px), et les taux de glutathion (GSH) re´duit. On a divise´ des rats maˆles en 3 groupes : ayant subi une ope´ration factice (sham) (n = 12), I/R (n = 12) et I/R avec 3AB (I/R–3- AB) (n = 12). On a occlus l’arte`re testiculaire gauche pendant 1 h, puis on l’a soumis a` une reperfusion pendant 24 h (pour les examens biochimiques et histopathologiques) et 30 jours (pour l’analyse de sperme). L’administration de 3-AB par voie intrape´ritone´ale 10 min avant et 1 h apre`s la reperfusion a augmente´ la diminution induite par l’I/R de la motilite´ du sperme dans les 2 testicules, et elle a diminue´ l’augmentation anormale des taux de sperme dans le testicule ipsilate´ral. Toutefois, le traitement par 3-AB n’a pu pre´venir la diminution induite par l’I/R du taux de sperme dans les 2 testicules. Les activite´s de SOD et de CAT sont demeure´es stables chez tous les groupes. L’I/R a augmente´ l’activite´ de la GSH-Px et les taux de GSH. Le traitement par 3-AB a renverse´ l’augmentation induite par l’I/R de l’activite´ de la GSH-Px comme chez les sham, mais il n’a pas modifie´ les taux de GSH. Le traitement par 3-AB a augmente´ de manie`re significative la diminution induite par l’I/R du score histopathologique. En conclusion, un traitement par 3-AB offre des avantages biochimiques et histopathologiques potentiels au-dela` de la qualite´ du sperme et pourrait diminuer l’alte´ration de la torsion testiculaire.Öğe The effects of statins on testicular ischemia reperfusion-induced histopathologic injury(2012) Kurçer, Zehra; Özbakış, Günnur Dengiz; Baba, Füsun; Banoğlu, Zekiye NurAmaç: Testiküler torsiyon infertiliteye neden olabilen yaygın bir sendromdur. Bu çalışma farklı farmakolojik özelliklere sahip statinlerin, testiküler torsiyon/detorsiyonu yansıtan bir model olan testiküler iskemi/reperfüzyon (IR) hasarı modelinde, tek dozlarının histopatolojik etkilerini değerlendirmek için planlandı. Gereç ve Yöntem: Sıçanlar operasyondan önce intraperitonal (i.p.) tiyopental sodyum (50 mg/kg) ile anestezi edildi. Sıçanların sol testiküler arter ve venleri 1 saat süreyle oklüze edildikten sonra, 24 saat reperfüze edildi. Histolojik değerlendirme için orşiektomi yapıldı. Simvastatin (5 mg/kg, i.p.), pravastatin (5 mg/kg, i.p.), atorvastatin (10 mg/kg, i.p.) veya çözücü (% 10 dimethilsülfoksit) reperfüzyondan önce uygulanıldı. Bulgular: IR sol testislerde belirgin histopatolojik hasara neden oldu. IR grubunun ortalama Johnsen skoru sham grubundan daha düşüktü (p0.001). Simvastatinle tedavi edilmiş testislerin skoru sham grubundan daha düşük (p0.001), fakat IR grubundan daha yüksek bulundu (p0.004). Atorvastatin ve pravastatin tedavileri ortalama Johnsen skorunu değiştirmedi. Sonuç: Bu çalışma, IR ile oluşturulan histopatolojik hasarı, tek doz uygulanan simvastatin tedavisinin azalttığını; tek doz atorvastatin ve pravastatin tedavilerinin ise değiştirmediğini göstermektedir. Sonuç olarak, statinlerin sergilemiş olduğu bu farklı etkilerin, onların farklı kimyasal ve farmakolojik özelliklerine bağlı olabileceğini düşünmekteyiz.Öğe Gastrointestinal Stromal Tumor Mimicking Ovarian Tumor(2012) Kerimoğlu, Özlem Seçilmiş; Baba, Füsun; Tazegül, Aybike; Alptekin, Hüsnü; Acar, Hasan; Çelik, ÇetinMalignant gastrointestinal stromal tumors are rare mesenchymal tumors originating from the gastroin- testinal tract. Making a differential diagnosis with gynecologic masses may be difficult in the preopera- tive period. The patient was a 45- year-old woman who presented to our clinic with a palpable mass fill- ing the pelvis. The CA 125 value was 42.7 U/ml. She underwent right hemicolectomy, total omentectomy, total abdominal hysterectomy, bilateral salpingo-oophorectomy and bilateral pelvic and para-aortic lymph node dissection since the frozen section assessment of the biopsy from the right colon was re- ported as a tumoral mass probably originating from the ovary. Tumoral tissue showed diffuse cytoplas- mic membraneous staining immunohistochemically with CD117, showed strong focal strong cytoplasmic staining with SMA. It was stained negative with desmin, S-100 and CD34. Molecular analysis of KIT gene was performed, we didn't detect mutations of exon 9 or 11. Surgical exploration of pelvic masses with undetermined origins should be performed in multidiciplinary hospitals including gynecological on- cology and surgical oncology teams. Histopathological examinations should be performed by experi- enced pathologistsÖğe Tübero sklerozlu hastada bilateral renal anjiomyolipom sebebi ile masif retroperitoneal kanama(2009) Kılıç, Özcan; Yurdakul, Talat; Kaynar, Mehmet; Özbek, Orhan; Baba, FüsunRenal anjiomyolipom (AML), tüberoskleroz kompleksinin (TS) en yaygın ürolojik bulgusudur. Bu kitleler daha çok kanamayla iliskili önemli derecede morbiditeye sebep olabilirler. Burada, bilateral renal cerrahi uygulanan TS ile iliskili, iki tarafı ve çok sayıda renal AML’si bulunan hastayı sunuyoruz. Olgu, klinik bulgular ve tedavi yöntemleri esliğinde tartısılmıstır.
