Effects of 3-Aminobenzamide on Unilateral Testicular Ischemia-Reperfusion Injury: What is the Role of PARP Inhibition?
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Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
On a examine´ les effets the´rapeutiques de l’inhibition de la poly(ade´nosine diphosphate-ribose) polyme´rase par
le 3-aminobenzamide (3-AB) dans une le´sion d’ische´mie-reperfusion (I/R) des testicules en utilisant une analyse de
sperme. On a effectue´ des examens histopathologiques et biochimiques pour mesurer les activite´s de la superoxyde dismutase (SOD), de la catalase (CAT), de la glutathion peroxydase (GSH-Px), et les taux de glutathion (GSH) re´duit. On a divise´ des rats maˆles en 3 groupes : ayant subi une ope´ration factice (sham) (n = 12), I/R (n = 12) et I/R avec 3AB (I/R–3-
AB) (n = 12). On a occlus l’arte`re testiculaire gauche pendant 1 h, puis on l’a soumis a` une reperfusion pendant 24 h
(pour les examens biochimiques et histopathologiques) et 30 jours (pour l’analyse de sperme). L’administration de 3-AB
par voie intrape´ritone´ale 10 min avant et 1 h apre`s la reperfusion a augmente´ la diminution induite par l’I/R de la motilite´
du sperme dans les 2 testicules, et elle a diminue´ l’augmentation anormale des taux de sperme dans le testicule ipsilate´ral.
Toutefois, le traitement par 3-AB n’a pu pre´venir la diminution induite par l’I/R du taux de sperme dans les 2 testicules.
Les activite´s de SOD et de CAT sont demeure´es stables chez tous les groupes. L’I/R a augmente´ l’activite´ de la GSH-Px
et les taux de GSH. Le traitement par 3-AB a renverse´ l’augmentation induite par l’I/R de l’activite´ de la GSH-Px comme
chez les sham, mais il n’a pas modifie´ les taux de GSH. Le traitement par 3-AB a augmente´ de manie`re significative la diminution induite par l’I/R du score histopathologique. En conclusion, un traitement par 3-AB offre des avantages biochimiques et histopathologiques potentiels au-dela` de la qualite´ du sperme et pourrait diminuer l’alte´ration de la torsion
testiculaire.
The therapeutic effects of poly(adenosine diphosphate-ribose) polymerase inhibition by 3-aminobenzamide (3- AB) were investigated in testicular ischemia–reperfusion (I/R) injury, using sperm analysis and histopathological and biochemical examinations, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and reduced glutathione (GSH) levels. Male rats were divided into 3 groups: sham (n = 12), I/R (n = 12), and I/R with 3-AB (I/R–3-AB) (n = 12). The left testicular artery was occluded for 1 h, followed by 24 h (for biochemical and histopathological examinations) and 30 days (for sperm analysis) of reperfusion. 3-AB treatment intraperitoneally 10 min prior to and 1 h after reperfusion increased the I/R-induced decrease in sperm motility in both testes and reduced the increased abnormal sperm rates in the ipsilateral testis. However, 3-AB treatment failed to prevent the I/R-induced decrease in sperm concentration in both testes. SOD and CAT activities did not change in any group. GSH-Px activity and GSH levels were increased by I/R. 3-AB treatment reversed the I/R-induced increase in GSH-Px activity, similar to the level in sham rats, but did not alter GSH levels. 3-AB treatment significantly increased the I/R-induced decrease in histopathologic score. In conclusion, 3-AB treatment has potential biochemical and histopathological benefits beyond improving sperm quality and may have the potential to decrease damage from testicular torsion.
The therapeutic effects of poly(adenosine diphosphate-ribose) polymerase inhibition by 3-aminobenzamide (3- AB) were investigated in testicular ischemia–reperfusion (I/R) injury, using sperm analysis and histopathological and biochemical examinations, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and reduced glutathione (GSH) levels. Male rats were divided into 3 groups: sham (n = 12), I/R (n = 12), and I/R with 3-AB (I/R–3-AB) (n = 12). The left testicular artery was occluded for 1 h, followed by 24 h (for biochemical and histopathological examinations) and 30 days (for sperm analysis) of reperfusion. 3-AB treatment intraperitoneally 10 min prior to and 1 h after reperfusion increased the I/R-induced decrease in sperm motility in both testes and reduced the increased abnormal sperm rates in the ipsilateral testis. However, 3-AB treatment failed to prevent the I/R-induced decrease in sperm concentration in both testes. SOD and CAT activities did not change in any group. GSH-Px activity and GSH levels were increased by I/R. 3-AB treatment reversed the I/R-induced increase in GSH-Px activity, similar to the level in sham rats, but did not alter GSH levels. 3-AB treatment significantly increased the I/R-induced decrease in histopathologic score. In conclusion, 3-AB treatment has potential biochemical and histopathological benefits beyond improving sperm quality and may have the potential to decrease damage from testicular torsion.
Açıklama
Anahtar Kelimeler
Kaynak
Canadian Journal of Physiology and Pharmacology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
88
Sayı
12
Künye
Baba, F., Hekimoğlu, A., Kurçer, Z., Aral, F., Ateşşahin, A., Sakin, F., (2010). Effects of 3-Aminobenzamide on Unilateral Testicular Ischemia-Reperfusion Injury: What is the Role of PARP Inhibition?. Canadian Journal of Physiology and Pharmacology, 88(12), 1123-1129. Doi:10.1139/Y10-093
