Effects of 3-Aminobenzamide on Unilateral Testicular Ischemia-Reperfusion Injury: What is the Role of PARP Inhibition?

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dc.contributor.authorHekimoğlu, Aşkın
dc.contributor.authorKurçer, Zehra
dc.contributor.authorAral, Faruk
dc.contributor.authorBaba, Füsun
dc.contributor.authorAteşşahin, Ahmet
dc.contributor.authorSakin, Fatih
dc.date.accessioned2020-03-26T17:48:04Z
dc.date.available2020-03-26T17:48:04Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractOn a examine´ les effets the´rapeutiques de l’inhibition de la poly(ade´nosine diphosphate-ribose) polyme´rase par le 3-aminobenzamide (3-AB) dans une le´sion d’ische´mie-reperfusion (I/R) des testicules en utilisant une analyse de sperme. On a effectue´ des examens histopathologiques et biochimiques pour mesurer les activite´s de la superoxyde dismutase (SOD), de la catalase (CAT), de la glutathion peroxydase (GSH-Px), et les taux de glutathion (GSH) re´duit. On a divise´ des rats maˆles en 3 groupes : ayant subi une ope´ration factice (sham) (n = 12), I/R (n = 12) et I/R avec 3AB (I/R–3- AB) (n = 12). On a occlus l’arte`re testiculaire gauche pendant 1 h, puis on l’a soumis a` une reperfusion pendant 24 h (pour les examens biochimiques et histopathologiques) et 30 jours (pour l’analyse de sperme). L’administration de 3-AB par voie intrape´ritone´ale 10 min avant et 1 h apre`s la reperfusion a augmente´ la diminution induite par l’I/R de la motilite´ du sperme dans les 2 testicules, et elle a diminue´ l’augmentation anormale des taux de sperme dans le testicule ipsilate´ral. Toutefois, le traitement par 3-AB n’a pu pre´venir la diminution induite par l’I/R du taux de sperme dans les 2 testicules. Les activite´s de SOD et de CAT sont demeure´es stables chez tous les groupes. L’I/R a augmente´ l’activite´ de la GSH-Px et les taux de GSH. Le traitement par 3-AB a renverse´ l’augmentation induite par l’I/R de l’activite´ de la GSH-Px comme chez les sham, mais il n’a pas modifie´ les taux de GSH. Le traitement par 3-AB a augmente´ de manie`re significative la diminution induite par l’I/R du score histopathologique. En conclusion, un traitement par 3-AB offre des avantages biochimiques et histopathologiques potentiels au-dela` de la qualite´ du sperme et pourrait diminuer l’alte´ration de la torsion testiculaire.
dc.description.abstractThe therapeutic effects of poly(adenosine diphosphate-ribose) polymerase inhibition by 3-aminobenzamide (3- AB) were investigated in testicular ischemia–reperfusion (I/R) injury, using sperm analysis and histopathological and biochemical examinations, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and reduced glutathione (GSH) levels. Male rats were divided into 3 groups: sham (n = 12), I/R (n = 12), and I/R with 3-AB (I/R–3-AB) (n = 12). The left testicular artery was occluded for 1 h, followed by 24 h (for biochemical and histopathological examinations) and 30 days (for sperm analysis) of reperfusion. 3-AB treatment intraperitoneally 10 min prior to and 1 h after reperfusion increased the I/R-induced decrease in sperm motility in both testes and reduced the increased abnormal sperm rates in the ipsilateral testis. However, 3-AB treatment failed to prevent the I/R-induced decrease in sperm concentration in both testes. SOD and CAT activities did not change in any group. GSH-Px activity and GSH levels were increased by I/R. 3-AB treatment reversed the I/R-induced increase in GSH-Px activity, similar to the level in sham rats, but did not alter GSH levels. 3-AB treatment significantly increased the I/R-induced decrease in histopathologic score. In conclusion, 3-AB treatment has potential biochemical and histopathological benefits beyond improving sperm quality and may have the potential to decrease damage from testicular torsion.en_US
dc.description.sponsorshipDicle UniversityDicle University [08TF51]en_US
dc.description.sponsorshipThis study was financially supported by Dicle University Researching Project Unit (grant No. 08TF51). Language help was provided by American Journal Experts.en_US
dc.identifier.citationBaba, F., Hekimoğlu, A., Kurçer, Z., Aral, F., Ateşşahin, A., Sakin, F., (2010). Effects of 3-Aminobenzamide on Unilateral Testicular Ischemia-Reperfusion Injury: What is the Role of PARP Inhibition?. Canadian Journal of Physiology and Pharmacology, 88(12), 1123-1129. Doi:10.1139/Y10-093
dc.identifier.doi10.1139/Y10-093en_US
dc.identifier.endpage1129en_US
dc.identifier.issn0008-4212en_US
dc.identifier.issn1205-7541en_US
dc.identifier.issue12en_US
dc.identifier.pmid21164558en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1123en_US
dc.identifier.urihttps://dx.doi.org/10.1139/Y10-093
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24820
dc.identifier.volume88en_US
dc.identifier.wosWOS:000285556200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBaba, Füsun
dc.language.isoenen_US
dc.publisherCANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESSen_US
dc.relation.ispartofCanadian Journal of Physiology and Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleEffects of 3-Aminobenzamide on Unilateral Testicular Ischemia-Reperfusion Injury: What is the Role of PARP Inhibition?en_US
dc.typeArticleen_US
dcterms.subject3-aminobenzamide
dcterms.subjecttestis
dcterms.subjectischemia–reperfusion
dcterms.subjectsperm analysis
dcterms.subject3-aminobenzamide
dcterms.subjecttesticule
dcterms.subjectische´mie–reperfusion
dcterms.subjectanalyse de sperme

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