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Öğe Association between endocrinological, immunological and psychosocial variables in psoriasis patients(EDIZIONI MINERVA MEDICA, 2016) Bilgic, Ozlem; Bilgic, Ayhan; Sivrikaya, Abdullah; Selvi, Yavuz; Unlu, Ali; Altinyazar, Hilmi C.BACKGROUND: There is limited data concerning the relationship between psychosocial problems of psoriasis patients and the function of their hypothalamic-pituitary-adrenal (IPA) axis and immunologic markers. This study aimed to determine serum levels of basal cortisol and adrenocorticotropic hormone (ACTH) and circulating levels of various cytokines and chemokines and their association with psychological measures in psoriasis patients. METHODS: Serum concentrations of endocrinological and immunological variables were quantified, and psychiatric questionnaires were completed. RESULTS: In psoriasis patients, serum levels of ACTH, TNF-a, IL-6, IL-23, CCL-17, CCL-27, CCL-20 and CXCL-9, current psychiatric symptoms and childhood neglect scores were all higher than in controls. In addition, in psoriasis patients, physical neglect scores were related to lower basal cortisol, whereas recent stressful life events were related to higher IL-6, IL-23 and CCL-20 levels. CONCLUSIONS: The exposure to stressful life events in childhood and just before a flare-up of psoriasis may be related to altered function of the HPA axis and an immune dysregulation in psoriasis.Öğe Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis(WALTER DE GRUYTER GMBH, 2013) Vatansev, Husamettin; Ozturk, Bahadir; Yilmaz, Sema; Sivrikaya, Abdullah; Dagli, Mehmet; Kiyici, Aysel; Unlu, AliObjective: Rheumatoid arthritis (RA) is a chronic inflammatory disease and it is associated with premature atherosclerosis development. RA patients have multiple factors to develop premature atherosclerosis. Enhanced asymmetric dimethylarginine (ADMA) is considered as a risk factor for the development of atherosclerosis by inducing endothelial dysfunction. The aim of this study was to investigate the role of asymmetric dimethylarginine, arginine and citrulline levels in patients with RA and their relation to the disease activity parameters for possible role on the disease activity. Methods: 92 patients with RA and 34 healthy controls were included the study. Patients and control blood samples collected for ADMA, arginine and citrulline levels. ADMA, arginine and citrulline levels were measured by pre-column derivatization fluorescence HPLC method. CRP and ESR levels examined on the same day were taken from the patient records. Results: While ADMA levels significantly increased, arginine levels decreased in the patient group. There was no difference in citrulline levels between both groups. Only citrulline and arginine showed a weak positive correlation. ADMA, arginine, and citrulline levels showed no correlation with either biochemical parameters such as CRP or ESR, or disease activity or disease severity. Conclusion: Our study has shown that an increase in ADMA levels with the decrease in arginine levels suggests an increase in arginine turnover to ADMA. Increased ADMA levels can be used for an increased risk for premature development of atherosclerosis in patients with RA. Since high ADMA levels prevent the NO-dependent vasodilatation, a possible role of ADMA in development of premature atherosclerosis may be independent from inflammation.Öğe The effect of 3 ',4 '-dihydroxyflavonol on plasma oxidant and antioxidant systems in testis ischemia-reperfusion injury in rats(BIOINTERFACE RESEARCH APPLIED CHEMISTRY, 2018) Duman, Aysel; Mogulkoc, Rasim; Baltaci, Abdulkerim Kasim; Sivrikaya, AbdullahThe purpose of the present study was to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on plasma lipid peroxidation in experimental testicular torsion-detorsion. The study involved 60 Wistar-albino type male rats weighing 250-260 gr. The experiment groups were formed as follows: 1. Control; 2. Sham-control; 3.720 degrees-4 hours torsion; 4. 720 degrees-4 hours torsion+4 hours detorsion; 5. 720 degrees-4 hours torsion+DiOHF; 6. 720 degrees-4 hours torsion+DiOHF+4 hours detorsion; 7. 720 degrees-4 hours torsion+24 hours detorsion; 8. 720 degrees-4 hours torsion+DiOHF+24 hours detorsion. The animals in the experimental groups were anesthetized after the procedures and their blood samples were taken to determine the levels of plasma glutathione peroxidase (GPx), nitric oxide (NO), malondialdehyde (MDA), and erythrocyte glutathione (GSH). Among the study groups, group 5 was found to have the highest plasma glutathione peroxidase values (p<0.001). Groups 3 and 4, which were torsion and detorsion groups, had the lowest plasma GPx values (p<0.001). Plasma NO values were found be higher in groups 3 and 4 than all other groups (p<0.001). Groups 3, 4, and 7 had the highest plasma MDA levels (p<0.001). Erythrocyte GSH levels in groups 5 and 7 were significantly higher than the levels in other groups (p<0.001). The results of the study indicate that lipid peroxidation that increases in plasma during testis ischemia-reperfusion injury in rats is prevented by intra-peritoneal DiOHF administration.Öğe Effect of Selenium Supplementation on Lipid Peroxidation, Antioxidant Enzymes, and Lactate Levels in Rats Immediately After Acute Swimming Exercise(HUMANA PRESS INC, 2011) Akil, Mustafa; Gurbuz, Umit; Bicer, Mursel; Sivrikaya, Abdullah; Mogulkoc, Rasim; Baltaci, Abdulkerim KasimThe present study aims to evaluate the effect of selenium supplementation on lipid peroxidation and lactate levels in rats subjected to acute swimming exercise. Thirty-two adult male rats of Sprague-Dawley type were divided into four groups. Group 1, control; group 2, selenium-supplemented; group 3, swimming control; group 4, selenium-supplemented swimming group. The animals in groups 2 and 4 were supplemented with (i.p.) 6 mg/kg/day sodium selenite for 4 weeks. The blood samples taken from the animals by decapitation method were analyzed in terms of erythrocyte-reduced glutathione (GSH), serum glutathione peroxidase (GPx) and superoxide dismutase (SOD), and plasma malondialdehyde (MDA) and lactate using the colorimetric method, and serum selenium values using an atomic emission device. In the study, the highest MDA and lactate values were found in group 3, while the highest GSH, GPx and SOD values were obtained in group 4 (p < 0,001). Group 2 had the highest and group 3 had the lowest selenium levels (p < 0,001). Results of the study indicate that the increase in free radical production and lactate levels due to acute swimming exercise in rats might be offset by selenium supplementation. Selenium supplementation may be important in that it supports the antioxidant system in physical activity.Öğe Effect of zinc supplementation on the distribution of various elements in the serum of diabetic rats subjected to an acute swimming exercise(SPRINGER, 2011) Bicer, Mursel; Akil, Mustafa; Sivrikaya, Abdullah; Kara, Ersan; Baltaci, Abdulkerim Kasim; Mogulkoc, RasimThe present study aims to examine the effect of supplementation of zinc on the distribution of various elements in the sera of diabetic rats subjected to an acute swimming exercise. A total of 80 Sprague-Dawley-type adult male rats were equally allocated to one of eight groups: Group 1, general; Group 2, zinc-supplemented; Group 3, zinc-supplemented diabetic; Group 4, swimming control; Group 5, zinc-supplemented swimming; Group 6, zinc-supplemented diabetic swimming; Group 7, diabetic swimming; and Group 8, diabetes. The rats were injected with 40 mg/kg/day subcutaneous streptozotocin (STZ) twice, with a 24-h interval between two injections. Zinc was supplemented at a dose of 6 mg/kg/day (ip) for 4 weeks. Blood samples were collected at the end of the 4-week study, and serum levels of lead, cobalt, molybdenum, chrome, sulfur, magnesium, manganese, sodium, potassium, phosphorus, copper, iron, calcium, zinc, and selenium (mg/L) were determined with atomic emission. The lowest molybdenum, chrome, copper, iron, potassium, magnesium, sodium, phosphorus, lead, selenium, and zinc values were obtained in Group 7 and 8. These same parameters were higher in the swimming exercise group (Group 4), relative to all other groups. The values in zinc-supplemented groups were found lower than the values in Group 4, but higher than those in Group 6 and 7. The results obtained from the study demonstrate that acute swimming exercise and diabetes affect the distribution of various elements in the serum, while zinc supplementation can prevent the negative conditions associated with both exercise and diabetes.Öğe Effects of Aminoglycoside Antibiotics on Renal Antioxidants, Malondialdehyde Levels, and Some Serum Biochemical Parameters(Canadian Vet Med Assoc, 2003) Yazar, Enver; Elmas, Muammer; Altunok, Vahdettin; Sivrikaya, Abdullah; Öztekin, Esma; Birdane, Yavuz OsmanEffects of amikacin, gentamicin, kanamycin, and streptomycin on renal tissue superoxide dismutase, glutathione peroxidase, glutathione and malondialdehyde, serum creatinine, potassium, sodium, total protein, glucose, uric acid, and total bilirubin levels were investigated. All aminoglycoside antibiotics decreased renal tissue glutathione levels.Öğe The effects of ankaferd blood stopper on DNA damage and enzymes with paranchymal damaged rabbits(2017) Metin, Bayram; Menevse, Esma; Sivrikaya, Abdullah; Altinoks, Tamer; Arıkoglu, HilalAnkaferd blood stopper (ABS) is a medical product that is used in several injuries, dental operations, prevention of minor or major bleeding after spontaneous or surgerical operations and have anti-microbial, anti-inflamatory, anti-thrombin, anti-platelet, anti-atherosclerotic, anti-oxidants effects. The present study is aimed to evaluate the effects of ABS on 8-hydroxy-2?-deoxyguanosine (8-OHdG), superoxide dismutase (SOD), myeloperoxidase (MPO) levels over pleural adhesions in rabbits with pulmonary parenchymal damage.16 New Zelland species rabbits were divided in two groups such as control (n7) and study group (n7). One rabbit in each group died during anesthesia. In both groups, we performed wedge resections in equal size to the left lower lobes of all rabbits. No interventions were made on control group, whereas 5 puff’s (1 cc) ABS was performed to the resection area at study group. Tube thoracostomy that performed both groups were terminated postoperatively at 6th hour after drainage and air leakages follow up. Rabbits were sacrificed with anesthetics at postoperative 8th day. Lung tissues were collected for analyzing of 8-OHdG, SOD, MPO. The 8-OHdG levels were respectively 2.010.39 ng/ml in control group and, 0.380.12 ng/ml in study. The differences between study and control group were statistically important group (p0.001). SOD and MPO levels did not show any statistically importance in the groups. As a conclusion, we can say that oxidative DNA damage prevented by ABSÖğe Effects of fasting and preoperative feeding in children(W J G PRESS, 2009) Yurtcu, Muslim; Gunel, Engin; Sahin, Tahir Kemal; Sivrikaya, AbdullahAIM: To investigate whether children should undergo surgery without a long period of fasting after feeding. METHODS: Eighty children with inguinoscrotal disorders (aged 1-10 years) were studied prospectively. They were divided into eight groups that each contained 10 children who were fed normal liquid food (NLF) and a high-calorie diet (HCD) 2, 3, 4 and 5 h before surgery, in two doses at 6-h intervals. NLF was given to four groups and HCD to the other four. In all groups, glucose, prealbumin and cortisol levels in the blood were measured twice: just after oral feeding and just before the operation. After the establishment of adequate anesthesia, gastric residue liquid was measured with a syringe. RESULTS: Blood glucose levels in all patients fed NLF and HCD were high, except in patients in, the HCD-4 group. There was no significant difference in the blood prealbumin levels. There was a significant increase in the blood cortisol levels in the NLF-2 (14.4 +/- 5.7), HCD-2 (13.2 +/- 6.0), NLF-3 (10.9 +/- 6.4), and HCD-5 (6.8 +/- 5.7) groups (P < 0.05). CONCLUSION: The stress of surgery may be tolerated by children when they are fed up to 2 h before elective surgery. (C) 2009 The WJG Press and Baishideng. All rights reserved.Öğe Effects of vitamin e supplementation on antioxidation and lipid profiles of rats on diets supplemented with cholesterol and olive oil(ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, 2008) Kurtoglu, Firuze; Kurtoglu, Varol; Sivrikaya, AbdullahLipid peroxidation (LPO) has been identified as an important component of atherosclerosis. In this study, the effects of supplementation with cholesterol (0.5%), olive oil (5%) and vitamin E (0.05%) on erythrocyte glutathione (GSH), plasma malondialdehyde (MDA), total cholesterol, HDL-LDL cholesterol and triacylglycerol, brain and liver MDA and GSH concentrations of rats were investigated. A total of 50 Sprague-Dawley male rats aged 6 months, and of equal body weight were used and fed a standard ration ad libitum. Animals were housed in the University of Selcuk, Veterinary Faculty Experimental Animals Unit. The experiment lasted 60 days and there were five experimental groups as follows: 1. Control, 2. Cholesterol (0.5%), 3. Olive oil (5%), 4. Cholesterol plus vitamin E (0.05%), 5. Olive oil plus vitamin E (0.05%). At the end of the experiment, blood samples were taken by cardiac puncture and erythrocyte GSH, plasma MDA, cholesterol, HDL-LDL cholesterol, triacylglycerol and also GSH and MDA concentrations in brain and liver tissue of rats were spectrophotometrically determined. Supplementation of olive oil and cholesterol into rat diets (groups 2 and 3) caused significant differences in lipid parameters; HDL cholesterol concentrations were increased in the olive oil group and LDL cholesterol was lower than in the cholesterol fed group. Moreover, these decreases in LDL and triacylglycerol concentrations were more significant with vitamin E supplementation. The high plasma MDA concentrations showed that lipid peroxidation occurred in the olive oil group and the highest brain MDA concentrations were determined also in the olive oil group. These findings suggest that vitamin E addition may decrease the sensitivities of several oils to oxidation and that monounsaturated fatty acids in olive oil may decrease the incidence of atherosclerosis by regulating blood lipid profiles.Öğe Effects of Zinc Deficiency and Supplementation on Malondialdehyde and Glutathione Levels in Blood and Tissues of Rats Performing Swimming Exercise(Humana Press Inc, 2003) Öztürk, Ahmet; Baltacı, Abdülkerim Kasım; Moğulkoç, Rasim; Öztekin, Esma; Sivrikaya, Abdullah; Kurtoğlu, Erdal; Kul, AylinThe aim of the study was to investigate the effects of zinc deficiency and supplementation on lipid peroxidation and glutathione levels in blood and in some tissues of rats performing swimming exercise. Forty adult male Sprague-Dawley rats were divided into four groups: group 1, zinc-deficient consisted of swimming rats; group 2 consisted of zinc-supplemented swimming rats; groups 3 and 4 were the swimming and nonswimming controls, respectively. The levels of malondialdehyde and glutathione were measured after 4 wk of zinc-deficient or zinc-supplemented diet and 30 min of swimming exercise daily. The erythrocyte glutathione levels of groups 2 and 4 were significantly higher than those of groups 1 and 3 (p<0.01). The plasma malondialdehyde level of group 1 was significantly higher than all other groups. The glutathione levels in liver, kidney, striated muscle, and testes of group 2 were higher than in the other groups (p<0.01) and higher in kidney and striated muscle of group 3 than in groups 1 and 4 (p<0.01). The tissue malondialdehyde levels of striated muscle, liver, kidney, and testes of group 1 were significantly higher than for all other groups (p<0.01). Our findings suggest that both swimming exercise and zinc deficiency result in an increase of lipid peroxidation in tissues and that zinc supplementation prevents these alterations by the activation of the antioxidant system.Öğe Effects of Zinc Supplementation on the Element Distribution in Kidney Tissue of Diabetic Rats Subjected to Acute Swimming(HUMANA PRESS INC, 2012) Sivrikaya, Abdullah; Bicer, Mursel; Akil, Mustafa; Baltaci, Abdulkerim Kasim; Mogulkoc, RasimIn this study, we report the effect of zinc supplementation on the distribution of elements in kidney tissue of diabetic rats subjected to acute swimming exercise. Diabetes was induced by two subcutaneous injections of 40 mg/kg of streptozotocin within a 24-h period. Zinc was given intraperitoneally at a dose of 6 mg/kg per day for a period of 4 weeks. The rats (n = 80) were equally divided into eight study groups: controls, zinc-supplemented, swimming, diabetic, zinc-supplemented diabetic, zinc-supplemented swimming, diabetic swimming, and zinc-supplemented diabetic swimming. The levels of lead, cobalt, molybdenum, chromium, boron, magnesium, iron, copper, calcium, zinc, and selenium were determined in the kidney tissue samples by ICP-AES. Higher molybdenum, calcium, zinc, and selenium values were found in both swimming and nonswimming diabetic rats. Significantly higher iron values were found in swimming, diabetic, diabetic swimming, and zinc-supplemented diabetic swimming rats (p < 0.001). Diabetic, zinc-supplemented diabetic, diabetic swimming, and zinc-supplemented diabetic swimming rats had the highest copper values. These results show that zinc supplementation normalized the higher levels of molybdenum, calcium, selenium, and iron levels seen in diabetic rats, indicating that zinc may have a regulatory effect on element metabolism in kidney tissue.Öğe Elevated Urinary Methylmalonic Acid/creatinine ratio and Serum Sterol levels in Patients with Acute Ischemic Stroke(UNIV PRESS, 2018) Sivrikaya, Abdullah; Öztürk, Şerefnur; Ekmekci, Hakan; Sağlam, Aslıhan; Abuşoğlu, Sedat; Ünlü, AliIntroduction: Sitosterolemia, defined as phytosterolemia, is a rare autosomal recessive disease characterized by elevated blood sterol levels. Our aim was to investigate serum plant sterols, methylmalonic acid, vitamin B12, oxidized-LDL and homocysteine levels in ischemic and hemorrhagic stroke patients and healthy subjects. Material and Methods: 50 healthy subjects (without a family history of coronary artery disease) and 89 patients hospitalized in the Selcuk University neurology clinic or intensive care unit with a diagnosis of stroke were included in this study. Serum plant sterols, homocysteine and methylmalonic acid, oxidized-LDL, total cholesterol, triglycerides, HDL-Cholesterol and vitamin B12 levels were analyzed by gas chromatography-mass spectrometry, liquid-chromatography tandem mass spectrometry, commercially available ELISA kit, spectrophotometry and chemiluminescence methods, respectively. Results: Urinary methylmalonic acid/creatinine ratio (p< 0.05), serum beta-sitosterol levels and beta-sitosterol/cholesterol ratio were significantly higher (p < 0.01) in patients compared to the control group. There was a significant positive correlation between the serum OxLDL-methylmalonic acid, serum homocysteine-urinary methylmalonic acid/creatinine ratio, serum methylmalonic acid-Urinary methylmalonic acid (p< 0.05), serum homocysteine-urinary methylmalonic acid, urinary methylmalonic acid-methylmalonic acid/creatinine ratio, serum methylmalonic acid-methylmalonic acid/creatinine ratio, serum beta-sitosterol-beta-sitosterol/cholesterol, total cholesterol-HDL, total cholesterol-LDL (p < 0.01) levels and negative correlation between vitamin B12-serum methylmalonic acid (p< 0.05), cholesterol-stigmasterol/cholesterol, LDL-stigmasterol/cholesterol (p < 0.01) levels in the patient group. Conclusion: Our findings presented that the serum sitosterol levels were significantly higher in stroke patients compared to controls.Öğe Investigation of Plant Sterols, Oxidized Low Density Lipoproteins (LDL) and Homocysteine Levels in Patients with Coronary Arthery Disease and Healthy Controls(Nobel Ilac, 2010) Sivrikaya, Abdullah; Mehmetoğlu, İdris; Kurban, Sevil; Aygül, Nazif; Yerlikaya, F. HümeyraAmaç: Bu çalışma serum bitki sterollerinin (B- sitosterol, stigmasterol) koroner arter hastalığının gelişi- mindeki rolünü ve diğer potansiyel risk faktörleriyle korelasyonunu göstermek için yapıldı. Materyal ve Metod: Anjiografisi yapılan toplam 166 kişi koroner anjiografi sonuçlarına göre kontrol ve hasta olarak gruplandırıldı. Anjiografisi normal olan 60 kışı kontrol grubunu, üç majör koroner arterlerinin en az birinde > %50 stenozu olan 106 kişi hasta grubunu oluşturdu. Vakaların serum ß-sitosterol, stigmasterol, okside LDL ve homosistein düzeyleri analiz edildi Bulgular: Hastalarım serum ß-sitosterol, stigmasterol ve bunların kolesterole oranları, okside LDL ve homosistein düzeyleri kontrollere göre biraz yüksek (istatistiksel açıdan önemsiz) bulundu. Üç hastanın serum bitki sterol seviyelen diğerlerine göre çok yüksekti. Hasta grubunda ẞ-sitosterol ile kolesterol düzeyleri arasında, kontrol grubunda stigmasterol ile kolesterol düzeyleri arasında önemli pozitif korelasyon vardı. Serum bitki sterolleri ile bunların okside LDL, homo- sistein ve koroner oklüzyon seviyeleri arasında ise korelasyon yoktu. • Sonuç: Bulgularımız serum bitki sterollerinin koroner arter hastalarında bağımsız risk faktörü olmadığım göstermektedir. Aynca bu hastalarda diğer risk faktörlerinin yanında serum bitki sterollerinin de rutin olarak ölçülmesmin hastaların daha iyi değerlendirilmesinde çok önemli olacağı kanaatindeyiz.Öğe Is there any association between serum iron and copper levels in hemadialysis patients?(2009) Sivrikaya, Abdullah; Menevşe, Esma; Tiftik, Ali Muhtar; Tonbul, ZekiÇalışmanın amacı, hemodiyaliz hastalarında serum demir (Fe), bakır (Cu) ve ferritin düzeylerindeki değişiklikleri tespit etmek ve ferritin düzeyleri ile elementler arasında bir ilişkinin olup olmadığını belirtmekti. Çalışmaya 2-16 yıldır diyalize giren (yaş ortalaması 50.2616.36) 47 hemodiyaliz hastası (hemodiyaliz grubu) ve 23 sağlıklı kişi (yaş ortalaması 39.52ill.54) (kontrol grubu) dâhil edildi. Hemodiyaliz grubunda kan numuneleri diyaliz öncesi ve sonrası alındı. Veriler, gruplar arasında serum Fe düzeyleri bakımından önemli bir fark bulunmadığını gösterdi. Ancak, diyaliz öncesi Cu düzeyleri ve de diyaliz öncesi ve sonrası ferritin düzeyleri kontrol grubuna göre daha yüksekti (sırasıyla p0.05, p0.001). Diyaliz öncesi ferritin-Fe (r 0.373, p0.05) ve Fe-Cu (r 0.410, p0.01) düzeyleri arasında önemli pozitif korelasyon bulundu. Bu çalışmadan elde edilen bulgular, serum Cu düzeylerindeki değişikliklerin hemodiyaliz hastalarında önemli olabileceğini düşündürmektedir. Bununla birlikte, Cu ve Fe arasındaki korelasyon bu elementler arasında bir ilişkinin olduğunu göstermektedir. Fe ile Cu arasındaki ilişkinin aydınlanması için daha ileri çalışmalara ihtiyaç vardır.Öğe Pregnancy-associated plasma protein A and procalcitonin as markers of myocardial injury in patients with acute coronary syndrome(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2015) Bayir, Aysegul; Kara, Hasan; Kiyici, Aysel; Ozturk, Bahadir; Sivrikaya, Abdullah; Akyurek, FikretBackground/aim: To evaluate pregnancy-associated plasma protein A (PAPP-A), ischemia-modified albumin (IMA), procalcitonin, and troponin I levels as diagnostic markers of acute coronary syndrome in patients admitted to the emergency department. Materials and methods: The serum PAPP-A, IMA, procalcitonin, and troponin I levels were measured in 100 patients with acute coronary syndrome admitted to the emergency department and 100 healthy control subjects. Results: Patients with acute coronary syndrome had significantly greater mean serum PAPP-A (patients, 10 +/- 10 mIU/L; control subjects, 6 +/- 10 mIU/L; P <= 0.001), procalcitonin (patients, 2 +/- 10 mu g/L; control subjects, 0.4 +/- 2 mu g/L; P <= 0.001), and troponin I levels (patients, 6 +/- 8 mu g/L; control subjects, 0.2 +/- 0.3 mu g/L; P <= 0.001) than control subjects. There was no difference in mean IMA levels between patients and control subjects. There were no significant correlations between PAPP-A levels and IMA, procalcitonin, or troponin I levels in patients with acute coronary syndrome. Conclusion: The PAPP-A, procalcitonin, and troponin I levels were increased in patients with acute coronary syndrome. Therefore, elevated PAPP-A and procalcitonin levels, in addition to troponin I levels, may be useful markers of myocardial injury on admission to the emergency department.Öğe The Relations Between Levels of Cadmium and Thyroid Parameters in Hemodialysis Patients(2013) Sivrikaya, Abdullah; Menevşe, Esma; Altıntepe, Lütfullah; Tifti, Ali MuhtarAmaç: Sunulan çalışmanın amacı, hemodiyaliz ve sağlıklı kişilerde serum kadmiyum (Cd), total triiyodotironin (T3), total tiroksin (T4) ve adrenokor- tikotropik hormon (ACTH) düzeyleri arasında istatistiksel olarak farklılık ve de gruplar arasında bu eser element ile tiroit profilleri ve ACTH arasında korelasyon olup olmadığını değerlendirmekti. Gereç ve Yöntem: Çalışmaya 2-16 yıldır haftada en az üç kez diyalize giren, her seansı en az 4 saat olan 47 hemodiyaliz hastası (hemodiyaliz grubu) ve 23 sağlıklı kişi (kontrol grubu) dahil edildi. Hemodiyaliz grubunda kan numuneleri diyaliz öncesi ve sonrası alındı. Kontrol grubunun kan numuneleri 10 saatlik açlık sonrası alındı. Bul- gular: Total T3 (p0.001) düzeyleri kontrol grubunda diyaliz öncesi ve diyaliz sonrasına göre daha yüksekti. Total T3 (p0.05) düzeyleri diyaliz öncesi diya- liz sonrasına göre daha düşüktü. Kontrol grubunda Total T4 düzeyleri yüksek, Cd düzeyleri ise düşük tespit edildi. Bu parametreler, diyaliz öncesine göre ise, istatistiksel açıdan önemli farklılık (sırasıyla p0.001, p0.01) gösterdi. ACTH düzeyleri bakımından gruplar arasında önemli bir farklılık bulunmadı. Tartışma: Hemodiyaliz hastalarında Cd düzeylerinin ölçülmesinin önemli ola- bileceği kanısındayız. Ancak Cd, tiroit profilleri ve ACTH düzeyleri arasında önemli bir ilişki bulamadık.Öğe The relationship between erythrocyte zinc levels and isotretinoin use in acne vulgaris patients(TAYLOR & FRANCIS LTD, 2015) Bilgic, Ozlem; Altinyazar, Hilmi Cevdet; Sivrikaya, Abdullah; Unlu, AliBackground: Prior studies have demonstrated lower serum zinc levels in acne vulgaris (AV) patients compared with controls. However, no study has investigated the relationship between AV and erythrocyte zinc levels, which is a superior indicator of body zinc levels. Additionally, the potential influence of isotretinoin use on body zinc status remains to be evaluated. In this study, we aimed to determine erythrocyte zinc levels and their relationship with isotretinoin use in AV patients. Methods: The enrolled study participants included 106 (68% female) isotretinoin-treated AV patients, 89 (65% female) untreated AV patients and 100 (59% female) healthy volunteers between 18 and 30 years of age. The acne severity of the AV patients who did not receive treatment was assessed using the classification system of the International Consensus Conference on Acne. Erythrocyte zinc levels were analysed by atomic absorption spectroscopy. Results: No significant differences were observed among the three groups with respect to erythrocyte zinc levels. In addition, erythrocyte zinc levels did not vary according to the severity of AV, nor according to the duration of isotretinoin use. Conclusion: This study suggests that no relationships exist among zinc status, AV and isotretinoin use. However, given the relationship between vitamin A and zinc, and the fact that previous studies have indicated low serum zinc levels in AV patients, prospective studies are required to corroborate our data.Öğe The relationship between serum clozapine concentrations and hematological parameters by a validated mass spectrometric method(Elsevier B.V., 2020) Kamil Gharab, Karam Mazin; Onmaz, Duygu Eryavuz; Abuşoğlu, Sedat; Aydın, Memduha; Sivrikaya, Abdullah; Tok, Oğuzhan; Abuşoğlu, Gülsüm; Ünlü, AliObjective: Clozapine is one of the most effective drugs for resistant schizophrenia, but its severe metabolic and hematological side effects limit the use of clozapine. It has been reported that clozapine blood concentrations should be maintained between 350?600 ng/mL. Our aim was to develop a determination method for clozapine and its main metabolites norclozapine and clozapine-N-oxide, to perform validation studies and to investigate the change of various biochemical parameters in patients using clozapine. Methods: A liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed and validated for clozapine measurement. Thus, blood samples were collected from 38 patients with schizophrenia and 32 healthy volunteers. Biochemical and hematological parameters were measured by Beckman-Coulter AU 5800 (Beckman Coulter, Brea, USA) and Beckman Coulter LH 780 analyzer (Beckman Coulter, Miami, FL, USA), respectively. Hormone levels were analyzed using Cobas 6000 analyzer (Roche Diagnostics, Germany). Results: The LC[sbnd]MS/MS method was linear between 1.22?2500 ng/mL (r2 = 0.9971) for clozapine. The retention times of clozapine, norclozapine and clozapine-N-oxide were 0.92, 0.89 and 0.95, respectively. Blood glucose (GLU) (p = 0.025), low density lipoprotein (LDL-cholesterol) (p = 0.015), triglyseride (TG) (p = 0.042) and total cholesterol (TC) (p = 0.024) levels were higher; hemoglobin (HGB) (0.015), mean corpuscular hemoglobin (MCH) (0.036), red blood cell count (RBC) (0.020), neutrophil (NEU) (0.034), and platelet (PLT) (P = 0.005) levels were lower in the clozapine group. Conclusions: This LC–MS/MS method was rapid, simple, cost-effective and suitable for the routine clozapine monitoring. Furthermore, norclozapine and clozapine-N-oxide were also determined. Monitoring of metabolic and hematological parameters with clozapine levels is very important. However, the limitations of the study were that the method was not validated for norclozapine and clozapine-N-oxide, so the validation parameters were not evaluated for these two metabolites. © 2019 Elsevier B.V.Öğe The Role of Prohepcidin and Hepcidin in Anemia Associated with Behcet's Disease(AKAD DOKTORLAR YAYINEVI, 2012) Dağlı, Mehmet; Yılmaz, Sema; Sivrikaya, Abdullah; Çelik, GülperiThe aim of the study is to investigate the role of circulating prohepcidin and hepcidin, which are homeostatic regulators of iron metabolism and mediators of inflammation, in anemia associated with Behcet's disease (BD) and the differential diagnosis of healthy controls. Twenty patients with BD and twenty healthy controls were included in this prospective study. Laboratory tests including. complete blood count, serum prohepcidin, hepcidin, iron, total iron binding capacity (TIBC), transferrin, ferritin, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were determined. Serum prohepcidin and hepcidin levels in patients with BD were significantly higher than healthy group. In the group with BD positive correlation was determined between the values of serum prohepcidin, hepcidin, hemoglobin, total binding capacity and ferritine. Serum prohepcidin and hepcidin levels are closely associated with disease activity in patients and might play a role in the pathobiology of chronic disease anemia associated with patients with BD.Öğe Romatoid artritli hastalarda asimetrik dimetilarjinin ve arjinin düzeyleri(2013) Vatansev, Hüsamettin; Öztürk, Bahadır; Yılmaz, Sema; Sivrikaya, Abdullah; Dağlı, Mehmet; Kıyıcı, Aysel; Ünlü, AliAmaç: Romatoid artrit (RA) ataklar ile seyreden kronik inflamatuar bir hastalıktır ve sıklıkla erken ateroskleroz gelişimi ile birliktedir. RA hastalarında erken ateroskleroz gelişimini hızlandıracak birçok faktör bulunmaktadır. Yüksek serum asimetrik dimetil arjinin (ADMA) düzeyleri endotel bütünlüğünü ve fonksiyonunu bozarak ateroskleroz gelişimini hızlandıran bağımsız bir faktör olarak kabul edilmektedir. Çalışmamızda RA hastalarında ADMA, arjinin ve sitrülin düzeylerini, hastalık aktivasyonundaki olası rolü içinde aktivite parametreleri ile ilişkisini araştırmayı amaçladık. Yöntemler: RA tanısı almış ve romatoloji kliniğinin takibinde olan 92 RA hastası ve bilinen herhangi bir hastalığı olmayan 34 sağlıklı kontrol çalışmaya dâhil edildi. Serum ADMA, arjinin ve sitrülin düzeyleri pre-kolon derivatizasyon-HPLC yöntemi ile saptandı. Rutin takip parametreleri olarak kullanılan eritrosit sedimantasyon hızı ve CRP değerleri hasta dosyasından alındı. Bulgular: ADMA düzeyleri RA hasta grubunda anlamlı derecede artarken, arjinin düzeylerinde azalma saptandı. Sitrülin seviyelerinde anlamlı bir değişikliğe rastlanmadı. Arjinin ve sitrülin arasında anlamlı zayıf bir korelasyon saptanırken, ADMA, arjinin ve sitrülin ile CRP, sedimantasyon hızı, hastalık şiddeti ve süresi arasında önemli bir ilişki saptanmadı. Sonuç: Bulgularımızda yüksek ADMA düzeylerinin azalan arjinin düzeyleri ile birlikte olmasının romatoid artritte artan arjinin-ADMA dönüşümünün bir göstergesi olabileceği yanında, bu hastalarda artan prematür ateroskleroz gelişiminde de rol oynayabileceğini göstermektedir. Yüksek ADMA düzeylerinin NO bağımlı vazodilatasyonu engellemesi nedeni ile prematür ateroskleroz gelişiminde, ADMA'nın rolünün inflamasyondan bağımsız olabileceğini düşündürmektedir