Immobilization of tyrosinase and alcohol oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole

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dc.contributor.authorYildiz, Huseyin Bekir
dc.contributor.authorSahmetlioglu, Ertugrul
dc.contributor.authorBoyukbayram, Ayse Elif
dc.contributor.authorToppare, Levent
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2020-03-26T17:17:32Z
dc.date.available2020-03-26T17:17:32Z
dc.date.issued2007
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractImmobilization of tyrosinase and alcohol oxidase is achieved in the copolymer of pyrrole with vinyl alcohol with thiophene side groups (PVATh-co-PPy) which is a newly synthesized conducting polymer. PVATh-co-PPy/alcohol oxidase and PVATh-co-PPy/tyrosinase electrodes are constructed by the entrapment of enzyme in conducting copolymer matrix during electrochemical copolymerization. For tyrosinase and alcohol oxidase enzymes, catechol and ethanol are used as the substrates, respectively. Kinetic parameters: maximum reaction rates (V-max) and Michaelis-Menten constants (K-m) are obtained. V-max and K-m are found as 2.75 mu mol/(min electrode) and 18 mM, respectively, for PVATh-coPPy/alcohol oxidase electrode and as 0.0091 mu mol/(min electrode) and 40 mM, respectively, for PVATh-co-PPy/tyrosinase electrode. Maximum temperature and pH values are investigated and found that both electrodes have a wide working range with respect to both temperature and pH. Operational and storage stabilities show that although they have limited storage stabilities, the enzyme electrodes are useful with respect to operational stabilities. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.ijbiomac.2007.04.006en_US
dc.identifier.endpage337en_US
dc.identifier.issn0141-8130en_US
dc.identifier.issue3en_US
dc.identifier.pmid17555810en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage332en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2007.04.006
dc.identifier.urihttps://hdl.handle.net/20.500.12395/21425
dc.identifier.volume41en_US
dc.identifier.wosWOS:000248355400016en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectconducting polymeren_US
dc.subjectelectrochemical polymerizationen_US
dc.subjectsensorsen_US
dc.subjectenzymesen_US
dc.subjectimmobilizationen_US
dc.subjectalcohol oxidaseen_US
dc.subjecttyrosinaseen_US
dc.subjectethanolen_US
dc.subjectpolyphenolsen_US
dc.titleImmobilization of tyrosinase and alcohol oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrroleen_US
dc.typeArticleen_US

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