Ozone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigs

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dc.contributor.authorOnal, Merih
dc.contributor.authorElsurer, Cagdas
dc.contributor.authorSelimoglu, Nebil
dc.contributor.authorYilmaz, Mustafa
dc.contributor.authorErdogan, Ender
dc.contributor.authorCelik, Jale Bengi
dc.contributor.authorKal, Oznur
dc.date.accessioned2020-03-26T19:42:15Z
dc.date.available2020-03-26T19:42:15Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe cochlea is an end organ, which is metabolically dependent on a nutrient and oxygen supply to maintain its normal physiological function. Cochlear ischemia and reperfusion (IR) injury is considered one of the most important causes of human idiopathic sudden sensorineural hearing loss. The aim of the present study was to study the efficacy of ozone therapy against cochlear damage caused by IR injury and to investigate the potential clinical use of this treatment for sudden deafness. Twenty-eight guinea pigs were randomized into four groups. The sham group (S) (n=7) was administered physiological saline intraperitoneally (i.p.) for 7 days. The ozone group (O) (n=7) was administered 1mg/kg of ozone i.p. for 7 days. In the IR + O group (n=7), 1 mg/kg of ozone was administered i.p. for 7 days before IR injury. On the eighth day, the IR + O group was subjected to cochlear ischemia for 15min by occluding the bilateral vertebral artery and vein with a nontraumatic clamp and then reperfusion for 2h. The IR group was subjected to cochlear IR injury. After the IR procedure, the guinea pigs were sacrificed on the same day. In a general histological evaluation, cochlear and spiral ganglionic tissues were examined with a light microscope, and apoptotic cells were counted by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The apoptotic index (AI) was then calculated. Blood samples were sent for analyses of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase, malondialdehyde (MDA), the total oxidant score (TOS), and total antioxidant capacity (TAC). Data were evaluated statistically using the Kruskal-Wallis test. The AI was highest in the IR group. The AI of the IR + O group was lower than that of the IR group. The biochemical antioxidant parameters SOD and GSH-Px and the TAC values were highest in the O group and lowest in the IR group. The MDA level and TOS were highest in the IR group and lowest in the O group. Controlled ozone administration stimulated endogenous antioxidant defense systems, thereby helping the body to combat IR injury. Although this study revealed a statistically significant decrease in cochlear IR damage following ozone therapy, further studies will be necessary to explain the protective mechanisms of ozone therapy in cochlear IR injury.en_US
dc.identifier.doi10.1111/aor.12863en_US
dc.identifier.endpage752en_US
dc.identifier.issn0160-564Xen_US
dc.identifier.issn1525-1594en_US
dc.identifier.issue8en_US
dc.identifier.pmid28261890en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage744en_US
dc.identifier.urihttps://dx.doi.org/10.1111/aor.12863
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35357
dc.identifier.volume41en_US
dc.identifier.wosWOS:000407272900007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofARTIFICIAL ORGANSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGuinea pigsen_US
dc.subjectCochleaen_US
dc.subjectOzoneen_US
dc.subjectIschemia-reperfusion injuryen_US
dc.subjectApoptosisen_US
dc.titleOzone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigsen_US
dc.typeArticleen_US

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