The effect of acrylamide on alpha-naphthyl acetate esterase enzyme in blood circulating lymphocytes and gut associated lymphoid tissues in rats

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dc.contributor.authorYener, Y.
dc.contributor.authorSur, E.
dc.contributor.authorTelatar, T.
dc.contributor.authorOznurlu, Y.
dc.date.accessioned2020-03-26T18:43:52Z
dc.date.available2020-03-26T18:43:52Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study is to determine the functional effects of the acrylamide (AA) administrated by oral gavage on the peripheral blood lymphocytes (PBL) and the Gut Associated Lymphoid Tissue (GALT) in male Sprague-Dawley rats using alpha-naphthyl acetate esterase (ANAE) demonstration. For this purpose, two separate experiments were performed with Sprague Dawley rats. In Experiment-I rats were gavaged with 0, 30,45 and 60 mg/kg b.w. AA for five consecutive days and in Experiment-II rats were gavaged with 0, 125, 150, and 175 mg/kg/b.w. AA for single oral dose. Animals were sacrificed 24 h after the last treatments in both experiments by servical dislocations under ether anaesthesia. Blood samples were collected from the heart in heparinized (IOU! heparin/ml(-1) of the blood) tubes before sacrification and lymphoid tissue samples from the ileal Peyer's patches (IPPs) were taken and processed for histochemical demonstration of ANAE following the sacrification. The lymphoid follicles of the IPPs of animals given 125, 150 and 175 mg/kg b.w. AA were markedly reduced in size. Germinal centres (GCs) markedly regressed in AA-treated animals compared with those of controls. ANAE-positive lymphocyte depletion of IPPs was very prominent in the high doses AA-treated animals. In the animals treated with 30, 45, and 60 mg/kg b.w. AA, the IPPs had similar histology to those of the controls. ANAE-positive peripheral blood lymphocyte levels significantly decreased in AA exposed groups in a dose dependent manner (p < 0.05). In conclusion, AA has detrimental effects on peripheral blood lymphocytes (PBL) and the Gut Associated Lymphoid Tissue (GALT) in rats. (C) 2011 Elsevier GmbH. All rights reserved.en_US
dc.identifier.doi10.1016/j.etp.2011.07.002en_US
dc.identifier.endpage146en_US
dc.identifier.issn0940-2993en_US
dc.identifier.issn1618-1433en_US
dc.identifier.issue01.02.2020en_US
dc.identifier.pmid21856137en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage143en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.etp.2011.07.002
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29902
dc.identifier.volume65en_US
dc.identifier.wosWOS:000314006000022en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER GMBHen_US
dc.relation.ispartofEXPERIMENTAL AND TOXICOLOGIC PATHOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAcrylamideen_US
dc.subjectANAEen_US
dc.subjectRaten_US
dc.titleThe effect of acrylamide on alpha-naphthyl acetate esterase enzyme in blood circulating lymphocytes and gut associated lymphoid tissues in ratsen_US
dc.typeArticleen_US

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