Use of a laser to detect viable but immotile spermatozoa

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dc.contributor.authorAktan, Tahsin Murad
dc.contributor.authorMontag, M
dc.contributor.authorDuman, Selçuk
dc.contributor.authorGörkemli, Hüseyin
dc.contributor.authorRink, Klaus
dc.contributor.authorYurdakul, Talat
dc.date.accessioned2020-03-26T16:55:38Z
dc.date.available2020-03-26T16:55:38Z
dc.date.issued2004
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA prerequisite for the success of intracytoplasmic sperm injection (ICSI) is the use of viable sperm that will support oocyte activation and embryo development. Usually, spermatozoa are selected according to motility, which is a clear indication for viability. However, in cases with complete asthenozoospermia, it is impossible to select viable sperm by morphological means. Here we report for the first time on the use of a laser to discriminate between viable and dead immotile spermatozoa. The application of a single laser shot to the far end of the sperm tail causes a curling of the sperm tail only in viable sperm, similar to the reaction observed in the hypo-osmotic swelling test. Using this method for sperm selection prior to ICSI we achieved high fertilization and cleavage rates in cases with immotile sperm in fresh testicular biopsy material as well in cases with ejaculated immotile sperm. It is concluded that laser sperm interaction allows for the identification of viable but immotile spermatozoa.en_US
dc.identifier.doi10.1111/j.1439-0272.2004.00636.xen_US
dc.identifier.endpage369en_US
dc.identifier.issn0303-4569en_US
dc.identifier.issue6en_US
dc.identifier.pmid15541052en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage366en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1439-0272.2004.00636.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/19276
dc.identifier.volume36en_US
dc.identifier.wosWOS:000225027600004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBLACKWELL VERLAG GMBHen_US
dc.relation.ispartofANDROLOGIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectasthenozoospermiaen_US
dc.subjecthumanen_US
dc.subjectimmotile spermatozoaen_US
dc.subjectintracytoplasmic sperm injectionen_US
dc.subjectlaseren_US
dc.subjectsperm viabilityen_US
dc.titleUse of a laser to detect viable but immotile spermatozoaen_US
dc.typeArticleen_US

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