Biological activities and DNA interactions of Amanita ovoidea

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dc.contributor.authorDogan, Hasan Huseyin
dc.contributor.authorArslan, Emine
dc.date.accessioned2020-03-26T19:01:07Z
dc.date.available2020-03-26T19:01:07Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractContext: Amanita ovoidea (Bull.) Link (Amanitaceae) is a well-known species due to its pleasant aroma and flavor since ancient times in the worldwide. This species is also known in Turkey and people consume it extensively. Objective: To evaluate medicinal importance of A. ovoidea for human health, to explain the effect of mushroom extracts on bacterial DNA, and to find preventive role on bacterial disease. Materials and methods: Chloroform, acetone, and methanol extracts of A. ovoidea were tested for the antimicrobial activities against four Gram-positive bacteria, five Gram-negative bacteria, and yeast using a micro-dilution method. In addition, DNA binding, DNA cleavage activity, and restriction enzyme digestion of the methanol extract of A. ovoidea were examined at different concentrations (40.000-78.125 mu g/mL). Results: The highest minimum inhibitory concentration (MIC) value observed against the test micro-organisms was with the chloroform extract (MIC 19.5 mg/mL concentration) against Candida albicans. Other highest antimicrobial effects observed against the test micro-organisms were with the methanol extracts against Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, Streptococcus pyogenes, Candida albicans, Klebsiella pneumoniae, Proteus vulgaris, and Salmonella enteritidis (MICs, 78 mu g/mL concentrations). All concentrations reduced the mobility of plasmid DNA. BamHI and HindIII targeted specially to supercoils and cut them. Amanita ovoidea extract prevented cutting with HindIII by binding especially to the AA region in open circular DNA. Discussion and conclusion: Present results demonstrated that A. ovoidea has excellent antimicrobial and antifungal activities by its DNA interaction activity on pBR322.en_US
dc.description.sponsorshipFoundation for TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG/109T584]; Scientific Research Projects (BAP) Coordinating Office at Selcuk UniversitySelcuk University [BAP/10401054]en_US
dc.description.sponsorshipThe authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. The authors are indebted to the Foundation for TUBITAK (TBAG/109T584) and Scientific Research Projects (BAP) Coordinating Office (BAP/10401054) at Selcuk University for their financial support of the current work.en_US
dc.identifier.doi10.3109/13880209.2014.982303en_US
dc.identifier.endpage1390en_US
dc.identifier.issn1388-0209en_US
dc.identifier.issn1744-5116en_US
dc.identifier.issue9en_US
dc.identifier.pmid25874387en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1386en_US
dc.identifier.urihttps://dx.doi.org/10.3109/13880209.2014.982303
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31893
dc.identifier.volume53en_US
dc.identifier.wosWOS:000361337700019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofPHARMACEUTICAL BIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAntimicrobial activityen_US
dc.subjectDNA bindingen_US
dc.subjectDNA cleavageen_US
dc.subjectmicro-dilutionen_US
dc.titleBiological activities and DNA interactions of Amanita ovoideaen_US
dc.typeArticleen_US

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