Improvement of catalytic activity of lipase from Candida rugosa via sol-gel encapsulation in the presence of calix(aza)crown

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dc.contributor.authorUyanık, Arzu
dc.contributor.authorŞen, Nejdet
dc.contributor.authorYılmaz, Mustafa
dc.date.accessioned2020-03-26T18:14:52Z
dc.date.available2020-03-26T18:14:52Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractLipase from Candida rugosa (CRL) was encapsulated within a chemically inert sol-gel support in the presence of calix(aza)crowns as the new additives. The catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenyl palmitate (p-NPP) and the enantioselective hydrolysis of racemic Naproxen methyl ester. It has been observed that the percent activity yields of the calix(aza)crown based encapsulated lipases were higher than that of the free lipase. Improved enantioselectivity was observed with the calix(aza)crown-based encapsulated lipases as compared to encapsulated free lipase. The reaction of Naproxen methyl ester resulted in 48.4% conversion for 24 h and 98% enantiomeric excess for the S-acid, corresponding to an E value of > 300 (E = 166 for the encapsulated free enzyme). Moreover, the encapsulated lipases were still retained about 18% of their conversion ratios after the sixth reuse in the enantioselective reaction. (C) 2010 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University, Konya, TurkeySelcuk University [08101022]en_US
dc.description.sponsorshipThis work has been funded by the Scientific Research Foundation of Selcuk University (BAP-Grant No: 08101022), Konya, Turkey. The authors also thank Shahabuddin Memon for his useful contribution.en_US
dc.identifier.doi10.1016/j.biortech.2010.12.105en_US
dc.identifier.endpage4318en_US
dc.identifier.issn0960-8524en_US
dc.identifier.issn1873-2976en_US
dc.identifier.issue6en_US
dc.identifier.pmid21256747en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4313en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.biortech.2010.12.105
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26547
dc.identifier.volume102en_US
dc.identifier.wosWOS:000288356300003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCI LTDen_US
dc.relation.ispartofBIORESOURCE TECHNOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectEnantioselective hydrolysisen_US
dc.subjectSol-gel encapsulationen_US
dc.subjectCalix(aza)crownsen_US
dc.subjectNaproxenen_US
dc.titleImprovement of catalytic activity of lipase from Candida rugosa via sol-gel encapsulation in the presence of calix(aza)crownen_US
dc.typeArticleen_US

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