Investigation of juglone effects on metastasis and angiogenesis in pancreatic cancer cells

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dc.contributor.authorAvci, Ebru
dc.contributor.authorArikoglu, Hilal
dc.contributor.authorKaya, Dudu Erkoc
dc.date.accessioned2020-03-26T19:24:49Z
dc.date.available2020-03-26T19:24:49Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractJuglone, a natural component, is shown to have cytotoxic and apoptotic effects in several cancer cell lines. However, little is known about its effects on invasion and metastasis. In this study, we aimed to determine the antimetastatic effect of juglone in the BxPC-3 and PANC-1 pancreatic cancer cell lines. Cytotoxic effect of juglone was evaluated by using MTT (3-(4,5-Dimethylthiazo1-2-yl)-2,5-Diphenyltetrazolium Bromide) test. The cells were treated with juglone at <IC50 doses (5, 10, 15 and 20 mu M) for 24 h. After the cell adhesion and invasion analysis, expression profiles of the MMP-2, MMP-9 and Phactr-1 genes were determined by OCR. The IC50 dose of juglone was found to be 21.05 mu M in the BxPC-3 cell line and 21.25 mu M in the PANC-1 cell line for 24 h. According to the cell adhesion and invasion analysis, treatment of juglone for 24 h reduced the adhesion and invasion features of pancreatic cancer cells. A significant reduction of MMP-2, MMP-9 and Phactr-1 expressions was observed in pancreatic cancer cells after the treatment of juglone at <IC50 doses. By this study, it has been shown for the first time that juglone inhibits cell invasion and metastasis in pancreatic cancer line and can be evaluated as an effective anticancer agent in pancreatic cancer. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipInstructor Training Programen_US
dc.description.sponsorshipThis study was produced from the MSc thesis of Ebru Avci and supported by Instructor Training Program.en_US
dc.identifier.doi10.1016/j.gene.2016.05.001en_US
dc.identifier.endpage78en_US
dc.identifier.issn0378-1119en_US
dc.identifier.issn1879-0038en_US
dc.identifier.issue1en_US
dc.identifier.pmid27155528en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage74en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.gene.2016.05.001
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33733
dc.identifier.volume588en_US
dc.identifier.wosWOS:000378370200010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofGENEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectJugloneen_US
dc.subjectMetastasisen_US
dc.subjectPancreatic cancer cell linesen_US
dc.titleInvestigation of juglone effects on metastasis and angiogenesis in pancreatic cancer cellsen_US
dc.typeArticleen_US

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