Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders

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dc.contributor.authorBuyukleblebici, Serhat
dc.contributor.authorTuncer, Purhan Barbaros
dc.contributor.authorBucak, Mustafa N.
dc.contributor.authorTasdemir, Umut
dc.contributor.authorEken, Ayse
dc.contributor.authorBuyukleblebici, Olga
dc.contributor.authorDurmaz, Emre
dc.date.accessioned2020-03-26T18:49:47Z
dc.date.available2020-03-26T18:49:47Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThere are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the dithiothreitol 5 mM or sucrose 25 mM, and control (without additives) was cooled to 4 degrees C and frozen in 0.25-ml French straws. when compared to control, different doses cryoprotectants and antioxidants addition no significantly increased the percentages of post-thaw sperm progressive and motitilities, acrosome abnormality and plasma membrane integrity (P > 0.05). However, EG3 + S yielded the greatest percentages of the total abnormality (P < 0.05). As regard to antioxidant activities G7 and EG5 led to lowest MDA activity with or without D or S but, these results were not supported to the GPx activity (P < 0.01). The sperm motion characteristics such as VAP, VCL, ALH and BCF gave significantly different results (P < 0.05). When compared the DNA integrity, different doses cryoprotectants without antioxidants addition significantly increased the percentages the tail intensity and tail moment (P < 0.05). There were no significant differences observed in non-return rates among all treatment groups (P > 0.05). (C) 2014 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipRepublic of Turkey, Ministry of Agriculture and Rural Affairs; General Directorate of Agricultural Research (GDAR)Gida Tarim Ve Hayvancilik Bakanligi [09/01/01/01]en_US
dc.description.sponsorshipThis study was supported by the Republic of Turkey, Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research (GDAR) Project number: 09/01/01/01.en_US
dc.identifier.doi10.1016/j.cryobiol.2014.05.005en_US
dc.identifier.endpage78en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.issue1en_US
dc.identifier.pmid24858399en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage74en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2014.05.005
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30684
dc.identifier.volume69en_US
dc.identifier.wosWOS:000340691700012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBovineen_US
dc.subjectAntioxidantsen_US
dc.subjectCryoprotectantsen_US
dc.subjectSperm parametersen_US
dc.subjectFreeze-thawingen_US
dc.titleComparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extendersen_US
dc.typeArticleen_US

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