Bone Morphogenetic Protein-7 Enhances Cementoblast Function In Vitro

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dc.contributor.authorHakkı, Sema S.
dc.contributor.authorFoster, Brian L.
dc.contributor.authorNagatomo, Kanako J.
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorHakkı, Erdoğan E.
dc.contributor.authorSomerman, Martha J.
dc.contributor.authorNohutcu, Rahime M.
dc.date.accessioned2020-03-26T17:47:17Z
dc.date.available2020-03-26T17:47:17Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Bone morphogenetic protein (BMP)-7 is a potent bone-inducing factor and was shown to promote periodontal regeneration in vivo and in vitro; however, to our knowledge, the specific effect of BMP-7 on cementoblasts has not been defined. We aimed to investigate the effects of BMP-7 on cementoblasts, which are cells responsible for tooth root-cementum formation. We hypothesized that BMP-7 would regulate mineralized tissue-associated genes in cementoblasts and influence the expression profile of genes associated with cementoblast extracellular matrix (ECM) and cell adhesion molecules (CAMs). Methods: A murine immortalized cementoblast cell line (OCCM.30) was cultured with and without 50 ng/ml BMP-7. After 72 hours, total RNA was isolated, and mRNA levels for bone/cementum markers, including bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN), and runt-related transcription factor-2 (Runx2), were investigated by real-time quantitative reverse transcription-polymerase chain reaction (Q-PCR). In vitro mineral nodule formation was assayed on day 8 using von Kossa staining. A pathway-specific gene-expression array was used to determine BMP-7-responsive ECM and CAM genes in cementoblasts. Results: Mineralized tissue markers were strongly regulated by BMP-7, with an almost three-fold increase in BSP and OCN transcripts and significant increases in OPN and Runx2 mRNA expressions. BMP-7 treatment markedly stimulated cementoblast-mediated biomineralization in vitro compared to untreated cells at day 8. BMP-7 treatment altered the OCCM.30 expression profile for ECM and CAM functional gene groups. BMP-7 tended to increase the expression of collagens and matrix metalloproteinases (MMPs), mildly decreased tissue inhibitors of MMPs (TIMPs), and had mixed regulatory effects on integrins. Using Q-PCR, selected array results were confirmed, including a significant BMP-7-induced increase in MMP-3 and a decrease in TIMP-2 mRNA expression. Conclusion: These results support the promising applications of BMP-7 in therapies aimed at regenerating periodontal tissues lost as a consequence of disease.en_US
dc.description.sponsorshipScientific Research Coordination Office, Selcuk UniversitySelcuk University [BAP/2002-190, BAP/2003-070]en_US
dc.description.sponsorshipThis study was performed in the Research Center, Faculty of Dentistry, Selcuk University, and supported by the Scientific Research Coordination Office, Selcuk University (BAP/2002-190, BAP/2003-070 to Dr. Hakki). The authors report no conflicts of interest related to this study.en_US
dc.identifier.citationHakkı, S. S., Foster, B. L., Nagatomo, K. J., Bozkurt, S. B., Hakkı, E. E., Somerman, M. J., Nohutcu, R. M., (2010). Bone Morphogenetic Protein-7 Enhances Cementoblast Function In Vitro. Journal of Periodontology, 81(11), 1663-1674. Doi: 10.1902/jop.2010.100074
dc.identifier.doi10.1902/jop.2010.100074en_US
dc.identifier.endpage1674en_US
dc.identifier.issn0022-3492en_US
dc.identifier.issn1943-3670en_US
dc.identifier.issue11en_US
dc.identifier.pmid20681807en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1663en_US
dc.identifier.urihttps://dx.doi.org/10.1902/jop.2010.100074
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24664
dc.identifier.volume81en_US
dc.identifier.wosWOS:000284244700018en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHakkı, Sema S.
dc.institutionauthorBozkurt, S. Buket
dc.institutionauthorHakkı, Erdoğan E.
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Periodontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBone morphogenetic protein 7en_US
dc.subjectCollagenen_US
dc.subjectDental cementumen_US
dc.subjectExtracellular matrixen_US
dc.subjectMatrix metalloproteinasesen_US
dc.subjectRegenerationen_US
dc.titleBone Morphogenetic Protein-7 Enhances Cementoblast Function In Vitroen_US
dc.typeArticleen_US

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