Cytotoxicity of hemostatic agents on the human gingival fibroblast

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dc.contributor.authorYalcin, M.
dc.contributor.authorBarutcigil, C.
dc.contributor.authorUmar, I.
dc.contributor.authorBozkurt, B. S.
dc.contributor.authorHakki, S. S.
dc.date.accessioned2020-03-26T18:41:25Z
dc.date.available2020-03-26T18:41:25Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBACKGROUND: Achieving of moisture control especially gingival bleeding control is great challenge in clinical practice. Various hemostatic agents and techniques have been promoted for bleeding control during dental operation. But few studies have focused on the cytotoxicity of hemostatic solutions. AIM: The aim of this study was to evaluate cytotoxic effect of hemostatic agents on human gingival fibroblast cells by using real-time cell analysis method. MATERIALS AND METHODS: Two hemostatic solutions, Hemoban (Sultan Healthcare, Hacken-sack, NJ, USA) and Hemasatic Solutions (W.P. Dental, Hamburg, Germany) that includes mainly aluminum chloride were used with different concentration. Gingival fibroblasts were isolated from gingival connective tissue during crown lengthening surgery of systemically healthy subjects. Gingival fibroblasts were maintained with Dulbecco's modified eagle medium containing 10% fetal bovine serum. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Mannheim, Germany, and ACEA Biosciences, San Diego, CA, USA) was used to evaluate cell survival. After seeding 200 mL of the cell suspensions into the wells (10,000 cells/well) of the E-plate 96, gingival fibroblasts were treated with hemostatic solutions (1/2, 1/4 and 1/8 dilutions) and monitored every 15 minutes for 72 hours. For the proliferation experiments, the statistical analyses used were 1-way analysis of variance (ANOVA) and Tukey HSD multiple comparisons tests. RESULTS: According to statistically analysis, when evaluated at 48 and 72 hours, there were significant differences between the cell indexes of the control and all hemostatic agents groups (p < 0.001). Agent reduced cell index value significantly when compared to untreated control group. CONCLUSIONS: The results indicate that using of Hemoban or Hemostatic Solutions as astringent solutions have a significant cytotoxic effect on gingival fibroblast cells.en_US
dc.identifier.endpage988en_US
dc.identifier.issn1128-3602en_US
dc.identifier.issue7en_US
dc.identifier.pmid23640448en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage984en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29347
dc.identifier.volume17en_US
dc.identifier.wosWOS:000325207600018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherVERDUCI PUBLISHERen_US
dc.relation.ispartofEUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHemostatic agenten_US
dc.subjectReal-time cell analyzeren_US
dc.subjectCytotoxicityen_US
dc.titleCytotoxicity of hemostatic agents on the human gingival fibroblasten_US
dc.typeArticleen_US

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