Nonadrenergic, Noncholinergic Responses of the Rabbit Detrusor Smooth Muscle and the Role of L-Arginine/Nitric Oxide Pathway in These Responses

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dc.contributor.authorŞahin, A. S.
dc.contributor.authorAtalık, K. E.
dc.contributor.authorDoğan, N.
dc.date.accessioned2020-03-26T16:36:17Z
dc.date.available2020-03-26T16:36:17Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn the present study, nonadrenergic, noncholinergic (NANC) responses of rabbit detrusor smooth muscle and the possible involvement of the L-arginine/nitric oxide (L-ARG/NO) pathway was investigated. In the presence of atropine (10(-6) M) and guanithidine (10(-5) M), frequency-response curves were obtained by stimulating tissue with 10-sec trains or increasing frequencies (1-10 Hz) with 3-min intervals between stimulations. Electrical field stimulation (EFS) evoked a biphasic response in rabbit detrusor smooth muscle, consisting of an initial contraction followed by relaxation. ATP desensitization significantly inhibited contractions. L-NAME(10(-5) M) increased the contractions by a maximum of 33 +/- 5% at 1 Hz, 37 +/- 5% at 2 Hz, 18 +/- 4% at 4 Hz. 20 +/- 3% at 8 Hz and 15 +/- 4% at 10 Hz. In detrusor preparations, exposure to L-NAME (N-G-nitro-L-arginine methyl ester, 10(-5) M) significantly reduced the maximal relaxation to electrical stimulation to 7 +/- 3% of the control. In the presence of L-ARG (10(-4) M), contractions induced by electrical field stimulation (EFS) at 1, 2, 4, 8 and 10 Hz were reduced by 20 +/- 4, 24 +/- 3, 25 +/- 3, 20 +/- 3 and 30 +/- 5%, respectively. Exposure to L-ARG (10(-4) M) significantly increased the maximal relaxation to electrical stimulation to 52 +/- 5% of the control. Exogenously applied ATP (10(-5)- 10(-2) M) to rabbit detrusor muscle resulted in contractions while sodium nitroprusside (10(-7)-10(-3) M) caused concentration-dependent relaxation. These results suggest that nitric oxide (NO) acts as an inhibitory NANC neurotransmitter in the rabbit detrusor smooth muscle. Additionally, NANC contractions mediated by ATP released from NANC nerves, may be masked by thr L-ARG/NO pathway in this tissue.en_US
dc.identifier.citationŞahin, A. S., Atalık, K. E., Doğan, N., (2000). Nonadrenergic, Noncholinergic Responses of the Rabbit Detrusor Smooth Muscle and the Role of L-Arginine/Nitric Oxide Pathway in These Responses. Methods and Findings in Experimental and Clinical Pharmacology, 22(1), 31-35. Doi: 10.1358/mf.2000.22.1.795821
dc.identifier.doi10.1358/mf.2000.22.1.795821en_US
dc.identifier.endpage35en_US
dc.identifier.issn0379-0355en_US
dc.identifier.issue1en_US
dc.identifier.pmid10791292en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage31en_US
dc.identifier.urihttps://dx.doi.org/10.1358/mf.2000.22.1.795821
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17231
dc.identifier.volume22en_US
dc.identifier.wosWOS:000086238300004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorŞahin, A. S.
dc.institutionauthorAtalık, K. E.
dc.institutionauthorDoğan, N.
dc.language.isoenen_US
dc.publisherProus Science, Saen_US
dc.relation.ispartofMethods and Findings in Experimental and Clinical Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectin vitroen_US
dc.subjectdetrusor (rabbit)en_US
dc.subjectnonadrenegic, noncholinergic (NANC) responsesen_US
dc.subjectL-arginine/nitric oxide (L-ARG/NO) pathwayen_US
dc.titleNonadrenergic, Noncholinergic Responses of the Rabbit Detrusor Smooth Muscle and the Role of L-Arginine/Nitric Oxide Pathway in These Responsesen_US
dc.typeArticleen_US

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