Esterified glucomannan improves aflatoxin-induced damage of sperm parameters during liquid storage of ram semen at 5 degrees C

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dc.contributor.authorAtaman, Mehmet Bozkurt
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorCoyan, Kenan
dc.date.accessioned2020-03-26T18:50:32Z
dc.date.available2020-03-26T18:50:32Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of the present work was to study the effects of aflatoxin (AF) on sperm parameters in rams, and to determine the protective efficiency of esterified glucomannan (EG) co-administered with AF up to 96 h of the liquid storage of ram semen at 5 degrees C. Thirty-two Merino rams (12-14 months old) were used. The animals were examined for their general health status. To ensure their adaptation to the environment and the new feeding regimen, a 15-day acclimatization programme was applied to the animals, prior to the start of the study. Experimental feeding was continued for ninety-two days. The experimental design consisted of four dietary treatments. The control group (C) was fed with commercial feed. The AF group was fed with commercial feed plus 250 mu g/day of total AF. The EG group received commercial feed plus 2 g/day of EG. The AF + EG group was given commercial feed plus 250 mu g/day of total AF and 2 g/day of EG. In the study, ejaculates were obtained from rams twice a week for 12 weeks, using an electro-ejaculator. After collected, the ejaculates were diluted with a skimmed milk extender, and stored at 5 degrees C. Sperm motility and rates of abnormal and nonviable spermatozoa were determined for the different treatment groups at 5 degrees C at 0, 24, 48, 72 and 96 h of liquid storage. During the first two weeks of the trial, the groups did not statistically differ from each other for sperm motility or rates of abnormal and nonviable spermatozoa at 0, 24, 48 and 96 h of storage. As from the third week, the short-term storage of semen produced statistically significant differences between the AF group and the other treatment groups for sperm parameters (p < 0.05). The administration of aflatoxin was observed to have reduced sperm motility and to have increased the rates of abnormal and nonviable spermatozoa in comparison to the control group (p < 0.05), while EG co-administered with AF was determined to have ameliorated the adverse effects of AF on sperm parameters, and this ameliorative effect continued throughout the short-term storage of semen. On the other hand, aflatoxin administration resulted in the deterioration of the sperm parameters in the following weeks, and the combined administration of EG + AF reversed this adverse effect, thus, bringing the sperm parameters closer to the values of the control group. This study demonstrated that, in rams, AF adversely affected sperm, biochemical and testis parameters, and that the combined administration of EG and AF reversibly improved these adverse effects. (C) 2014 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [107 O 866]en_US
dc.description.sponsorshipThis study was financed under a project supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 107 O 866).en_US
dc.identifier.doi10.1016/j.cryobiol.2014.03.007en_US
dc.identifier.endpage410en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.issue3en_US
dc.identifier.pmid24662029en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage405en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2014.03.007
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30830
dc.identifier.volume68en_US
dc.identifier.wosWOS:000336884100014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRam semenen_US
dc.subjectAflatoxinen_US
dc.subjectGlucomannanen_US
dc.subjectLiquid storageen_US
dc.titleEsterified glucomannan improves aflatoxin-induced damage of sperm parameters during liquid storage of ram semen at 5 degrees Cen_US
dc.typeArticleen_US

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