A comparison between two different automated total 25-hydroxyvitamin D immunoassay methods using liquid chromatography-tandem mass spectrometry

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dc.contributor.authorKoçak, Fatma Emel
dc.contributor.authorÖztürk, Bahadır
dc.contributor.authorIşıklar, Özben Özden
dc.contributor.authorGenç, Özlem
dc.contributor.authorÜnlü, Ali
dc.contributor.authorAltuntaş, İrfan
dc.date.accessioned2020-03-26T19:00:20Z
dc.date.available2020-03-26T19:00:20Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntroduction: Total 25-hydroxyvitamin D [25(OH)D] is the most reliable indicator of vitamin D status. In this study, we compared two automated immunoassay methods, the Abbott Architect 25-OH Vitamin D assay and the Roche Cobas Vitamin D total assay, with the liquid chromatography-tandem mass spectrometry (LC-MS/MS). Materials and methods: One hundred venous blood samples were randomly selected from routine vitamin D tests. Two of the serum aliquots were analyzed at the Abbott Architect i2000 and the Roche Cobas 6000's module e601 in our laboratory within the same day. The other serum aliquots were analyzed at the LC-MS/MS in different laboratory. Passing-Bablok regression analysis and Bland-Altman plot were used to compare methods. Inter-rater agreement was analyzed using kappa (kappa) analysis. Results: The Roche assay showed acceptable agreement with the LC-MS/ MS based on Passing-Bablok analysis (intercept: -5.23 nmol/L, 95% CI: -8.73 to 0.19; slope: 0.97, 95% CI: 0.77 to 1.15). The Abbott assay showed proportional (slope: 0.77, 95% CI: 0.67 to 0.85) and constant differences (intercept: 17.08 nmol/L; 95% CI: 12.98 to 21.39). A mean bias of 15.1% was observed for the Abbott and a mean bias of -14.1% was observed for the Roche based on the Bland-Altman plots. We found strong to nearly perfect agreement in vitamin D status between the immunoassays and LC-MS/MS. (kappa: 0.83 for Abbott, kappa: 0.93 for Roche) using kappa analysis. Conclusion: Both immunoassays demonstrated acceptable performance, but the Roche Cobas assay demonstrated better performance than the Abbott Architect in the studied samples.en_US
dc.identifier.endpage438en_US
dc.identifier.issn1330-0962en_US
dc.identifier.issn1846-7482en_US
dc.identifier.issue3en_US
dc.identifier.pmid26526462en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage430en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31748
dc.identifier.volume25en_US
dc.identifier.wosWOS:000362855400013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCROATIAN SOC MEDICAL BIOCHEMISTSen_US
dc.relation.ispartofBIOCHEMIA MEDICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subject25-Hydroxyvitamin Den_US
dc.subjectchromatographyen_US
dc.subjectimmunoassayen_US
dc.subjectmethodsen_US
dc.subjecttandem mass spectrometryen_US
dc.titleA comparison between two different automated total 25-hydroxyvitamin D immunoassay methods using liquid chromatography-tandem mass spectrometryen_US
dc.typeArticleen_US

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