A microarray based expression profiling of paclitaxel and vincristine resistant MCF-7 cells

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dc.contributor.authorKars, Meltem Demirel
dc.contributor.authorIseri, Ozlem Darcansoy
dc.contributor.authorGunduz, Ufuk
dc.date.accessioned2020-03-26T18:08:16Z
dc.date.available2020-03-26T18:08:16Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractResistance to the broad spectrum of chemotherapeutic agents in cancer cell lines and tumors has been called multiple drug resistance (MDR). In this study, the molecular mechanisms of resistance to two anticancer agents (paclitaxel and vincristine) in mammary carcinoma cell line MCF-7 were investigated. Drug resistant sublines to paclitaxel (MCF-7/Pac) and vincristine (MCF-7/Vinc) that were developed from sensitive MCF-7 cells (MCF-7/S) were used. cDNA microarray analysis was performed for the RNA samples of sensitive and resistant cells in duplicate experiments. GeneSpring GX 7.3.1 Software was used in data analysis. The results indicated that the upregulation of MDR] gene is the dominating mechanism of the paclitaxel and vincristine drug resistance. Additionally the upregulation of the genes encoding the detoxifying enzymes (i.e. GSTP1) was observed. Significant downregulation of apoptotic genes (i.e. PDCD2/4/6/8) and upregulation of some cell cycle regulatory genes (CDKN2A, CCNA2 etc.) was seen which may be in close relation to MDR in breast cancer. Drug resistant cancer cells exhibit different gene expression patterns depending on drug treatment, and each drug resistance phenotype is probably genetically different. Further functional studies are needed to demonstrate the complete set of genes contributing to the drug resistance phenotype in breast cancer cells. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG 3297]; Selcuk University, TurkeySelcuk Universityen_US
dc.description.sponsorshipWe gratefully acknowledge advises of Prof. Dr. Fikret Arpaci and Prof. Dr. Ali Ugur Ural This study was supported by TUBITAK (SBAG 3297) and Selcuk University Scientific Research Fund, Turkey.en_US
dc.identifier.doi10.1016/j.ejphar.2011.02.001en_US
dc.identifier.endpage9en_US
dc.identifier.issn0014-2999en_US
dc.identifier.issn1879-0712en_US
dc.identifier.issue01.03.2020en_US
dc.identifier.pmid21320484en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ejphar.2011.02.001
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26059
dc.identifier.volume657en_US
dc.identifier.wosWOS:000290742400002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofEUROPEAN JOURNAL OF PHARMACOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMCF-7en_US
dc.subjectMultiple drug resistanceen_US
dc.subjectMicroarray analysisen_US
dc.subjectPaclitaxelen_US
dc.subjectVincristineen_US
dc.titleA microarray based expression profiling of paclitaxel and vincristine resistant MCF-7 cellsen_US
dc.typeArticleen_US

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