The molecular characterization of arcanobacterium pyogenes strains isolated from samples of sheep and cattle

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dc.contributor.authorHadimli, H. Huseyin
dc.contributor.authorKav, Kursat
dc.date.accessioned2020-03-26T18:17:02Z
dc.date.available2020-03-26T18:17:02Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümüen_US
dc.description.abstractThe purpose of this study was to determine the presence of virulence genes, perform typing for the characterization of Arcanobacterium pyogenes field strains, and investigate the correlation between clonal types, virulence genes and occurence of disease. The isolates (n=51) used in this study were isolated from different sources (liver (n=4) of sheep; liver (n=25), lungs (n=5), broncho-alveolar lavage (BAL) fluid (n=3), milk (n=12) and suppurative tissue (n=1) of dairy cows and synovial fluid (n=1) of a calf). The presence of haemolytic activity in A. pyogenes isolates was determined using rabbit, sheep, cattle, chicken, dog and human erythrocytes. Also, the presence of any cytotoxic effect was investigated by growth in Vero cell cultures. Genomic DNA fingerprinting for clonal analysis was generated by BOX-PCR typing. Conventional PCR was used for the determination of the presence of eight A. pyogenes virulence factor genes, namely, nanH (encoding neuraminidase H), nanP (encoding neuraminidase P), plo (encoding pyolysin-PLO), cbpA (encoding collagen-binding protein A) and fimA, fimC, fimE and fimG (encoding the major fimbrial subunit of four different fimbriae). Furthermore, the correlation between clonal types, virulence factors and the occurence of disease was investigated. The haemolysins of all strains had haemolytic effect on rabbit, sheep, cattle, chicken, dog and human erythrocytes. In addition, all strains were found to be cytotoxic to Vero cells. According to clonal analysis results, the A. pyogenes isolates were determined to belong to 12 different types. While all A. pyogenes strains were positive for the plo gene, the positivity rate was 62% for the nanH gene, 84% for the nanP gene, 58% for the cbp gene, 96% for the fimA gene, 66% for the fimC gene, 42% for the fimE gene and 10% for the fimG gene. It was determined that no correlation existed between the clonal types and virulence factors of A. pyogenes isolates and occurence of disease.en_US
dc.identifier.citationHadimli, H. H., Kav, K. (2011). The Molecular Characterization of Arcanobacterium pyogenes Strains Isolated from Samples of Sheep and Cattle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(6), 893-899.
dc.identifier.endpage899en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue6en_US
dc.identifier.startpage893en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26967
dc.identifier.volume17en_US
dc.identifier.wosWOS:000298308700003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorHadimli, H. Huseyin
dc.institutionauthorKav, Kursat
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectArcanobacterium pyogenesen_US
dc.subjectClonal typingen_US
dc.subjectVirulence genesen_US
dc.subjectMolecular characterizationen_US
dc.titleThe molecular characterization of arcanobacterium pyogenes strains isolated from samples of sheep and cattleen_US
dc.typeArticleen_US

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