Assessment of In vitro Antibacterial Activity and Cytotoxicity Effect of Nigella sativa Oil

Basit Öğe Kaydı
dc.contributor.authorUgur, Ayse Ruveyda
dc.contributor.authorDagi, Hatice Turk
dc.contributor.authorOzturk, Bahadir
dc.contributor.authorTekin, Gulsum
dc.contributor.authorFindik, Duygu
dc.date.accessioned2020-03-26T19:23:08Z
dc.date.available2020-03-26T19:23:08Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Methicillin resistance is a serious health concern since it has spread among Staphylococcus aureus and coagulase-negative Staphylococci (CoNS) that are frequent community and nosocomial pathogens worldwide. Methicillin-resistant strains are often resistant to other classes of antibiotics, making their treatment difficult. Nigella sativa oil is known to be active against Gram-positive cocci, yet its in vitro cytotoxicity is rarely investigated, is a proper and powerful candidate for treatment of methicillin-resistant isolates. Objectives: The aim of this study is to evaluate the in vitro antibacterial activity and cytotoxicity effect of N. sativa oil. Materials and Methods: The minimal inhibitory concentrations (MICs) of N. sativa oil were determined by broth microdilution method against four different American Type Culture Collection strains, 45 clinical isolates of methicillin-resistant S. aureus (MRSA), and 77 methicillin-resistant CoNS (MRCoNS). The effects of different dilutions (0.25 mu g/mL, 0.5 mu g/mL, and 1 mu g/mL) of N. sativa oil on the proliferation of gingival fibroblasts were evaluated. Results: The MIC values of N. sativa oil against clinical isolates of Staphylococci were between < 0.25 mu g/mL and 1.0 mu g/mL. Compared to the control group, there was no cytotoxic effect on the proliferation of the gingival fibroblasts. Conclusion: In the present study, the oil of N. sativa was very active against MRSA and MRCoNS and had no in vitro cytotoxicity at relevant concentrations. These findings emphasize that there is a requirement for further clinical trials on N. sativa oil for "safe" medical management of infections caused by methicillin-resistant Staphylococci.en_US
dc.identifier.doi10.4103/0973-1296.191459en_US
dc.identifier.endpageS474en_US
dc.identifier.issn0973-1296en_US
dc.identifier.issn0976-4062en_US
dc.identifier.issue47en_US
dc.identifier.pmid27761077en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpageS471en_US
dc.identifier.urihttps://dx.doi.org/10.4103/0973-1296.191459
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33283
dc.identifier.volume12en_US
dc.identifier.wosWOS:000391129400014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMEDKNOW PUBLICATIONS & MEDIA PVT LTDen_US
dc.relation.ispartofPHARMACOGNOSY MAGAZINEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCytotoxic effecten_US
dc.subjectmethicillin-resistant Staphylococcien_US
dc.subjectmicrodilutionen_US
dc.subjectNigella sativaen_US
dc.titleAssessment of In vitro Antibacterial Activity and Cytotoxicity Effect of Nigella sativa Oilen_US
dc.typeArticleen_US

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