Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Küçük Resim Yok

Tarih

2011

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

AMER SOC MICROBIOLOGY

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Cefquinome has a broad spectrum of antibacterial activity and was developed especially for use in animals. A simple and sensitive high-performance liquid chromatography (HPLC) method with UV-visible detection for quantification of cefquinome concentrations in sheep plasma was developed and validated. Separation of cefquinome from plasma components was achieved on a Phenomenex Gemini C-18 column (250 mm by 4.6 mm; internal diameter [i.d.], 5 mu m). The mobile phase consisted of acetonitrile and 0.1% trifluoroacetic acid in water and was delivered at a rate of 0.9 ml/min. A simple and rapid sample preparation involved the addition of methanol to 200 mu l of plasma to precipitate plasma proteins followed by direct injection of 50 mu l of supernatant into the high-performance liquid chromatography system. The linearity range of the proposed method was 0.02 to 12 mu g/ml. The intraday and interday coefficients of variation obtained from cefquinome were less than 5%, and biases ranged from -3.76% to 1.24%. Mean recovery based on low-, medium-, and high-quality control standards ranged between 92.0 and 93.9%. Plasma samples were found to be stable in various storage conditions (freeze-thaw, postpreparative, short-term, and long-term stability). The method described was found to be readily available, practicable, cheap, rapid, sensitive, precise, and accurate. It was successfully applied to the study of the pharmacokinetics of cefquinome in sheep. This method can be very useful and an alternate to performing pharmacokinetic studies in the determination of cefquinome for clinical use.

Açıklama

Anahtar Kelimeler

Kaynak

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

WoS Q Değeri

Q1

Scopus Q Değeri

Q1

Cilt

55

Sayı

2

Künye