The geographical distribution and first molecular analysis of Culicoides Latreille (Diptera: Ceratopogonidae) species in the Southern and Southeastern Turkey during the 2012 outbreak of bovine ephemeral fever

Basit Öğe Kaydı
dc.contributor.authorDik, B.
dc.contributor.authorMuz, D.
dc.contributor.authorMuz, M. N.
dc.contributor.authorUslu, U.
dc.date.accessioned2020-03-26T18:58:37Z
dc.date.available2020-03-26T18:58:37Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study investigated the geographical distribution and molecular analysis of Culicoides species in the Southern and Southeastern Turkey during the 2012 outbreak of bovine ephemeral fever (BEF). The midge specimens caught by Onderstepoort-type light traps from livestock farms were tested for molecular evidence of existence of viral genome. Blood specimens were collected from clinically BEF-suspected acute febrile cattle. Total nucleic acid samples obtained from field specimens were checked against the BEF virus G gene and Culicoides internal transcribed spacer 1 (ITS-1) gene. A total of 20,845 Culicoides specimens (20,569 a (TM) Euroa (TM) Euro, 276 a (TM),a (TM),) comprising 11 species (Culicoides badooshensis, Culicoides circumscriptus, Culicoides gejgelensis, Culicoides imicola, Culicoides kibunensis, Culicoides longipennis, Culicoides newsteadi, Culicoides nubeculosus, Culicoides odiatus, Culicoides punctatus, Culicoides schultzei, Culicoides spp.) were collected. C. schultzei (18,032) was found as the dominant species and followed by C. imicola (1,857), C. nubeculosus complex (545), and C. circumscriptus (259), respectively. C. kibunensis was identified as new species for this region. PCR positivity of BEF was found 37.14 % (13/35) in blood samples whereas no viral genome was obtained from Culicoides specimens. Culicoides spp. ITS-1 gene sequences were analyzed phylogenetically with GenBank ITS-1 sequences. Molecular homology of Culicoides ITS-1 gene was ranged between 62.74 and 71.39 %. The results described first molecular detection and phylogenetic analysis of Culicoides ITS-1 gene with reference to the 2012 BEF outbreak in Turkey.en_US
dc.description.sponsorshipSelcuk University Scientific Research CouncilSelcuk University [12401031]en_US
dc.description.sponsorshipThis research was partially funded by Selcuk University Scientific Research Council, res. no: 12401031. Researchers thank to Prof. Dr. Osman Erganis supply of BEFV as positive control.en_US
dc.identifier.doi10.1007/s00436-014-4098-zen_US
dc.identifier.endpage4232en_US
dc.identifier.issn0932-0113en_US
dc.identifier.issn1432-1955en_US
dc.identifier.issue11en_US
dc.identifier.pmid25199552en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4225en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00436-014-4098-z
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31210
dc.identifier.volume113en_US
dc.identifier.wosWOS:000343914500036en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofPARASITOLOGY RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCulicoidesen_US
dc.subjectITS-1 geneen_US
dc.subjectBovine ephemeral fever virusen_US
dc.subjectInsect vectoren_US
dc.subjectTurkeyen_US
dc.titleThe geographical distribution and first molecular analysis of Culicoides Latreille (Diptera: Ceratopogonidae) species in the Southern and Southeastern Turkey during the 2012 outbreak of bovine ephemeral feveren_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu