Evaluation and Comparison of Three Different Anti-hepatitis C Virus Antibody Tests based on Chemiluminescence and Enzyme-linked Immunosorbent Assay Methods used in the Diagnosis of Hepatitis C Infections in Turkey

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dc.contributor.authorKesli, R.
dc.contributor.authorOzdemir, M.
dc.contributor.authorKurtoglu, M. G.
dc.contributor.authorBaykan, M.
dc.contributor.authorBaysal, B.
dc.date.accessioned2020-03-26T17:38:36Z
dc.date.available2020-03-26T17:38:36Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe routine diagnosis of hepatitis C virus (HCV) infection is based on the detection of anti-HCV antibodies by two main methods (enzyme immunoassay [EIA] and chemiluminescence immunoassay [CIA]) but false-positives are a problem. We investigated three anti-HCV tests: two CIAs (Cobas (R) e 601 and Architects i2000SR); and one EIA (Ortho (R) HCV 3.0). Two other anti-HCV tests were also performed as supplementary and confirmatory tests, respectively: a recombinant strip immunoblot assay (RIBA HCV 3.0 SIA) and a reverse transcriptase polymerase chain reaction-based assay for HCV-RNA. After discriminating the false-positive results, the true anti-HCV seropositivity rate in 7156 serum samples was 0.91%. The seropositivity and false-positive rates for the Cobas (R) e 601, Architects i2000SR and Ortho (R) HCV 3.0 anti-HCV tests were 1.9% and 0.99%, 1.2% and 0.29%, and 0.87% and 0.01%, respectively. The mean level of HCV-RNA was 3399 x 10(3) IU/ml. Critical levels for false-positivity for HCV-RNA were a cut-off index of 200 for Cobas (R) e 601, a signal/cut-off (S/CO) of 5 for Architects i2000SR and an S/CO of 1.2 for Ortho (R) HCV 3.0. Positive and negative results for the RIBA HCV 3.0 SIA assay all accorded with the HCV-RNA assay, except for 23 (17%) 'indeterminate' results, all of which were negative with the HCV-RNA assay. In conclusion, to eliminate doubts related to false-positive findings in the initial HCV screening tests, additional confirmatory HCV-RNA assay should be performed.en_US
dc.identifier.doi10.1177/147323000903700516en_US
dc.identifier.endpage1429en_US
dc.identifier.issn0300-0605en_US
dc.identifier.issn1473-2300en_US
dc.identifier.issue5en_US
dc.identifier.pmid19930846en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1420en_US
dc.identifier.urihttps://dx.doi.org/10.1177/147323000903700516
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23526
dc.identifier.volume37en_US
dc.identifier.wosWOS:000273133200016en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSAGE PUBLICATIONS LTDen_US
dc.relation.ispartofJOURNAL OF INTERNATIONAL MEDICAL RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHEPATITIS C VIRUS (HCV) INFECTIONSen_US
dc.subjectANTI-HCV TESTSen_US
dc.subjectHCV-RNA ASSAYen_US
dc.subjectRECOMBINANT IMMUNOBLOT ASSAY (RIBA)en_US
dc.subjectFALSE-POSITIVE RESULTSen_US
dc.titleEvaluation and Comparison of Three Different Anti-hepatitis C Virus Antibody Tests based on Chemiluminescence and Enzyme-linked Immunosorbent Assay Methods used in the Diagnosis of Hepatitis C Infections in Turkeyen_US
dc.typeArticleen_US

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