BoLA-DRB3.2 polymorphism in Holstein friesian, Simmental and Brown swiss cattle breeds

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dc.contributor.authorIlhan, Fatma.
dc.contributor.authorAytekin, Ibrahim.
dc.date.accessioned2020-03-26T20:12:47Z
dc.date.available2020-03-26T20:12:47Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Ziraat Fakültesi, Zootekni Bölümüen_US
dc.description.abstractIn this research, the BoLA-DRB3 exon 2 region in the MHC gene region was surveyed by PCR-RFLP analysis in three of the cattle breeds that are commonly reared in Turkey. This locus was PCR-amplified, and a 284-bp fragment was obtained. To determine the variation in the BoLA-DRB3.2 gene region, BstYI and HaeIII restriction enzymes were used. Three genotypes and two alleles were detected as a result of BstYl enzyme restriction; six genotypes and three alleles were detected at the end of the HaeIII enzyme fragment. The bb genotype is more frequent in all breeds as a result of BstYI restriction. Chi-square analysis revealed that the population with this enzyme was in the Hardy-Weinberg equilibrium. For the HaeIII enzyme, the bb genotype had the highest frequency, and the population is not balanced according to the enzyme cutting results. The HaeIII restriction site at 167 bp correlated with the Pro 56 amino acid. This amino acid was included in the antigen-recognition site of the MHC molecule, which was recorded in 38% of Holstein Friesian, 22% of Simmental and 19% of Brown Swiss animals. This study of BoLA-DRB3 genes in cattle breeds grown in Turkey demonstrates the genetic profile.en_US
dc.description.sponsorshipScientific Research Project Coordinating Office of Selcuk University [16401048]en_US
dc.description.sponsorshipThis project was supported by a grant from The Scientific Research Project Coordinating Office of Selcuk University. (Project No: 16401048).en_US
dc.identifier.citationIlhan, F., Aytekin, I. (2019). BoLA-DRB3. 2 polymorphism in Holstein Friesian, Simmental and Brown Swiss Cattle Breeds. Fresenius Environmental Bulletin, 28(8), 6085-6089.
dc.identifier.endpage6089en_US
dc.identifier.issn1018-4619en_US
dc.identifier.issn1610-2304en_US
dc.identifier.issue8en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage6085en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37544
dc.identifier.volume28en_US
dc.identifier.wosWOS:000480511400044en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorIlhan, Fatma.
dc.institutionauthorAytekin, Ibrahim.
dc.language.isoenen_US
dc.publisherPARLAR SCIENTIFIC PUBLICATIONS (P S P)en_US
dc.relation.ispartofFRESENIUS ENVIRONMENTAL BULLETINen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBoLA-DRB3en_US
dc.subjectBrown Swissen_US
dc.subjectCattleen_US
dc.subjectHolstein Friesianen_US
dc.subjectPCR-RFLPen_US
dc.subjectSimmentalen_US
dc.titleBoLA-DRB3.2 polymorphism in Holstein friesian, Simmental and Brown swiss cattle breedsen_US
dc.typeArticleen_US

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