Expression profiles of interferon-tau stimulated genes (isgs) in peripheral blood leucocytes (pbls) and milk cells in pregnant dairy cows

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dc.contributor.authorKose, Mehmet
dc.contributor.authorGorgulu, Murat
dc.contributor.authorKaya, Mehmet Salih
dc.contributor.authorAydilek, Nurettin
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorBayril, Tahir
dc.contributor.authorKurar, Ercan
dc.date.accessioned2020-03-26T18:50:43Z
dc.date.available2020-03-26T18:50:43Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümüen_US
dc.description.abstractIn previous reports, it was indicated that measurement of activity of Interferon-tau Stimulated Genes (ISGs) in Peripheral Blood Leucocytes (PBLs) may be used as an alternative early pregnancy detection method in dairy cows. However, there are no data showing the expression profiles of ISGs in other body fluids containing leucocytes such as milk. In the present study, it was hypothesized that leucocytes in milk samples may reflect the increases in expression profiles of ISGs as shown in PBLs. For this purpose, nine pregnant lactating Holstein cows were used. Insemination day was accepted as day zero (day 0). Blood and milk samples were collected on day 0 and 18 after insemination for cell isolation. Total RNA was extracted from isolated cells and converted to cDNA. Steady state levels of Interferon-tau Stimulated Gene 15 (ISG15), Myxovirus (influenza virus) resistance 1 (MX1) and 2 (MX2) mRNA transcripts were assayed by using real-time reverse transcriptase PCR. Relative Expression Software Tool (REST2009) was used for statistical analyses. There was no statistical significant difference for expression levels of ISG15, MX1 and MX2 mRNAs between days 0 and 18 in milk samples. However, when compared to day 0, levels of ISG15 and MX2 transcripts were increased 6.97 +/- 0.68 fold and 5.84 +/- 1.27 fold on day 18 in PBLs in pregnant cows, respectively (P<0.05). According to this result, it may be suggested that milk cells are not suitable measurement of expression profiles of ISGs to detect early pregnancy in lactating dairy cows.en_US
dc.description.sponsorshipDicle University DUBAPDicle University [10-VF-43]en_US
dc.description.sponsorshipThis study was supported by Dicle University DUBAP - (10-VF-43)en_US
dc.identifier.citationKöse, M., Görgülü, M., Kaya, M. S., Aydilek, N., Bozkaya, F., Bayrıl, T., Kurar, E., Kıyma, Z., Güzeloğlu, A., Atlı, M. O. (2014). Expression Profiles of Interferon-Tau Stimulated Genes (ISGs) in Peripheral Blood Leucocytes (PBLs) and Milk Cells in Pregnant Dairy Cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20(2), 189-194.
dc.identifier.doi10.9775/kvfd.2013.9776en_US
dc.identifier.endpage194en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage189en_US
dc.identifier.urihttps://dx.doi.org/10.9775/kvfd.2013.9776
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30860
dc.identifier.volume20en_US
dc.identifier.wosWOS:000333080000004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKurar, Ercan
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEarly pregnancy detectionen_US
dc.subjectISGsen_US
dc.subjectqPCRen_US
dc.subjectMilken_US
dc.subjectCowen_US
dc.titleExpression profiles of interferon-tau stimulated genes (isgs) in peripheral blood leucocytes (pbls) and milk cells in pregnant dairy cowsen_US
dc.typeArticleen_US

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