Determination of Ochratoxin A in Poultry Feed by High-Performance Liquid Chromatography with a Monolithic Column
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
TAYLOR & FRANCIS INC
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
A monolithic column with high-performance liquid chromatography coupled with an ultraviolet detector was investigated for the determination of ochratoxin A in poultry feed. A systematic study was performed using solid phase extraction with a C-18 cartridge for sample pretreatment with three solvent systems. Ethyl acetate:methanol:formic acid (95:5:0.5) was found to be the most suitable. Pretreated samples were injected separately into packed and monolithic columns. The effects of the mobile phases on the chromatographic figures of merits were evaluated. Better peak symmetry, improved separation, and more theoretical plates were observed using an acetonitrile:water:formic acid (99:99:2) mobile phase. The repeatability and accuracy of the method were statistically evaluated and found to be satisfactory with a limit of detection of 40 mu gL(-1). The use of a monolithic column in conjunction with sample pretreatment provided good results for the determination of ochratoxin A in poultry feed.
Description
Keywords
Monolithic column, Poultry feed, Ochratoxin A, High-performance liquid chromatography, Ultraviolet detector
Journal or Series
ANALYTICAL LETTERS
WoS Q Value
Q4
Scopus Q Value
Q3
Volume
48
Issue
3
