The mechanism of growth-promoting effects of prolactin in embryogenesis - Links to growth factors

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dc.contributor.authorKarabulut, AK
dc.contributor.authorLayfield, R
dc.contributor.authorPratten, MK
dc.date.accessioned2020-03-26T16:27:24Z
dc.date.available2020-03-26T16:27:24Z
dc.date.issued1999
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe polypeptide hormone prolactin (PRL) has been implicated in the regulation of embryonic growth and development, but the control mechanisms involved in the effects of the hormone are poorly understood. Several investigators suggested that there may be a possible link between the effects of PRL and insulin-like growth factors (IGFs). Recent studies have also shown that ligand-induced activation of PRL receptors leads to tyrosine phosphorylation of multiple intracellular proteins, and tyrosine kinase activation takes place in mediating the mitogenic action of PRL. In order to determine whether IGFs are involved in mediating the growth-promoting effect of PRL, rat embryos were culture in vitro for 48 h in whole rat serum and serum depleted of low molecular weight molecules (30 kD retenate) supplemented with rat PRL in the presence and absence of antisera against rat PRL, IGF I and IGF II. To investigate the effects of inhibiting the signal transduction of the PRL receptors, the embryos were preincubated for 2 h in retenate in the presence of tyrosine kinase inhibitors, tyrphostin 47 and genistein, then rat PRL was added to the culture medium. Embryos cultured in retenate showed severe growth retardation, and the addition of rat PRL caused significant increase in growth and development of the embryos suggesting that embryos may be able to utilize maternally derived PRL during organogenesis. The presence of antiserum against rat PRL abolished the PRL-induced increase in development and antibodies against IGF I and II had a similar effect,, suggesting that IGFs may be involved in the effect of the hormone. The 2-hour preincubation with genistein and tyrphostin also abolished the PRL-induced increase in development. These results indicate that functional PRL receptors are present in rat embryos at this stage which may play an important role in the control of growth and development and this may be linked to growth factors and their receptors.en_US
dc.identifier.doi10.1159/000016637en_US
dc.identifier.endpage13en_US
dc.identifier.issn1422-6405en_US
dc.identifier.issn1422-6421en_US
dc.identifier.issue1en_US
dc.identifier.pmid10940668en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2en_US
dc.identifier.urihttps://dx.doi.org/10.1159/000016637
dc.identifier.urihttps://hdl.handle.net/20.500.12395/16991
dc.identifier.volume164en_US
dc.identifier.wosWOS:000080616700002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKARGERen_US
dc.relation.ispartofCELLS TISSUES ORGANSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectprolactinen_US
dc.subjectinsulin-like growth factorsen_US
dc.subjectembryo cultureen_US
dc.subjectgenisteinen_US
dc.subjectdevelopmenten_US
dc.subjectraten_US
dc.titleThe mechanism of growth-promoting effects of prolactin in embryogenesis - Links to growth factorsen_US
dc.typeArticleen_US

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