Gene Methylation of SFRP2, P16, DAPKI, HIC1, and MGMT and KRAS Mutations in Sporadic Colorectal Cancer

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dc.contributor.authorPehlivan, Sacide
dc.contributor.authorArtaç, Mehmet
dc.contributor.authorSever, Tuğçe
dc.contributor.authorBozcuk, Hakan
dc.contributor.authorKılınçarslan, Can
dc.contributor.authorPehlivan, Mustafa
dc.date.accessioned2020-03-26T17:48:54Z
dc.date.available2020-03-26T17:48:54Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the methylation of the SFRP2, P16, DAPK1, HIC1, and MGMT genes, as well as the mutation of amino acid codons 12 and 13 of the KRAS gene in normal and tumor tissue DNA of patients diagnosed with sporadic colorectal cancer (SCRC). The methylation of gene regions and the KRAS mutations of normal (N) and tumor tissue (T) DNA obtained from 17 patients diagnosed with SCRC and 20 healthy controls were investigated using the polymerase chain reaction and reverse-hybridization methods. There was an Asp mutation in four patients, an Asp and Ser mutations in one patient in codon 12 of the KRAS gene, and an Asp mutation in codon 13 in eight patients. Overall promoter methylation (OPM) in the SFRP2 gene was observed in one N and four T, whereas partial promoter methylation (PPM) was observed in two N and five T. OPM in the P16 gene was present in one T. In the DAPK1 gene, OPM existed in seven T and five N, while PPM was present in two N. In the HIC1 gene, OPM was demonstrated in three T, while PPM was noted in two N; however, no methylation existed in N. In the MGMT gene, OPM occurred in five T and two N, and PPM was present in one T. KRAS mutations in Turkish patients with SCRC are similar to those of other population groups. Methylations in the genes, which underwent methylation analysis, were higher in Tin comparison with N, and it has been suggested that significant results would be obtained by making a study with a larger population.en_US
dc.identifier.citationPehlivan, S., Artaç, M., Sever, T., Bozcuk, H., Kılınçarslan, C., Pehlivan, M., (2010). Gene Methylation of SFRP2, P16, DAPKI, HIC1, and MGMT and KRAS Mutations in Sporadic Colorectal Cancer. Cancer Genetics and Cytogenetics, (201), 128-132. Doi: 10.1016/j.cancergencyto.2010.05.019
dc.identifier.doi10.1016/j.cancergencyto.2010.05.019en_US
dc.identifier.endpage132en_US
dc.identifier.issn0165-4608en_US
dc.identifier.pmid20682398en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage128en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cancergencyto.2010.05.019
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24958
dc.identifier.volume201en_US
dc.identifier.wosWOS:000281108100009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorArtaç, Mehmet
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofCancer Genetics and Cytogeneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleGene Methylation of SFRP2, P16, DAPKI, HIC1, and MGMT and KRAS Mutations in Sporadic Colorectal Canceren_US
dc.typeArticleen_US

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