Identification and Expression of Babesia ovis Secreted Antigen 1 and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

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dc.contributor.authorSevinc, Ferda
dc.contributor.authorCao, Shinuo
dc.contributor.authorXuan, Xuenan
dc.contributor.authorSevinc, Mutlu
dc.contributor.authorCeylan, Onur
dc.date.accessioned2020-03-26T19:06:05Z
dc.date.available2020-03-26T19:06:05Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn order to identify immunoreactive proteins that are usable for the immunological diagnosis of Babesia ovis infections, a phage lambda cDNA expression library was constructed and screened using parasite-specific immune serum. Immunoscreening resulted in the identification of a full-length cDNA clone encoding a secreted protein designated Babesia ovis secreted antigen 1 (BoSA1). The full-length BoSA1 cDNA contained a 1,137-bp open reading frame that encoded a protein of 378 amino acids, with a signal peptide and 2 internal repeat domains. The theoretical molecular mass of the mature protein was 42.5 kDa. Recombinant BoSA1 (rBoSA1) protein was expressed in Escherichia coli strain DH5 alpha cells as a glutathione S-transferase (GST) fusion protein and was purified by affinity chromatography. Purified rBoSA1 was tested for reactivity with sera from animals experimentally or naturally infected with B. ovis, in an indirect enzyme-linked immunosorbent assay (ELISA). The results showed that specific antibodies against rBoSA1 were detectable on days 7 and 8 of the experimental infection and were maintained during the sampling period. Additionally, 38 field sera taken from sheep naturally infected with B. ovis gave strong positive reactions in the ELISA between day 20 and day 30 of treatment. As a result, the identified recombinant BoSA1 protein seems to be a promising diagnostic antigen that is usable for the development of serological assays for the diagnosis of ovine babesiosis. This is the first report on the molecular cloning, expression, and potential use of a recombinant antigen for the diagnosis of ovine babesiosis.en_US
dc.description.sponsorshipScientific and Technological Research Council in TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113O336]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council in Turkey (grant number 113O336).en_US
dc.identifier.doi10.1128/JCM.03219-14en_US
dc.identifier.endpage1536en_US
dc.identifier.issn0095-1137en_US
dc.identifier.issn1098-660Xen_US
dc.identifier.issue5en_US
dc.identifier.pmid25694531en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1531en_US
dc.identifier.urihttps://dx.doi.org/10.1128/JCM.03219-14
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32212
dc.identifier.volume53en_US
dc.identifier.wosWOS:000353036500011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAMER SOC MICROBIOLOGYen_US
dc.relation.ispartofJOURNAL OF CLINICAL MICROBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleIdentification and Expression of Babesia ovis Secreted Antigen 1 and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assayen_US
dc.typeArticleen_US

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