Characterization of Klebsiella pneumoniae Strains Isolated from Urinary Tract infections: Detection of ESBL Characteristics, Antibiotic Susceptibility and RAPD Genotyping

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dc.contributor.authorAladağ, Mustafa Onur
dc.contributor.authorUysal, Ahmet
dc.contributor.authorDündar, Niyazi
dc.contributor.authorDurak, Yusuf
dc.contributor.authorGüneş, Erdoğan
dc.date.accessioned2020-03-26T18:41:15Z
dc.date.available2020-03-26T18:41:15Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. The random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K. pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were detected among the strains. It was found that 100 K pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests were conducted using a disc diffusion method against 16 antibiotics. Fifty-five different resistance profiles were determined among the strains. ESBL-productions of the strains were determined by the double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates among the strains were found to be 55% by E-test method and 45% by DDST method. While ESBL-producing K pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%),the resistance rates of non ESBL producing strains to those antibiotics were determined as 97.8%, 88.8% and 88.8%, respectively. Both groups of strains showed the highest sensitivity to meropenem. Based on the results obtained from the study, it was concluded that the detection of ESBL-producing strains by the E-test method was more sensitive than by the DDST method. Phenotypic and genotypic identification methods should be used together to detect ESBL presence. The RAPD-PCR method alone will not be adequate in the genotyping of the strains and alternative DNA-based methods should be used.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordinating Office (BAP)Selcuk University [10401050]en_US
dc.description.sponsorshipWe would like to thank Selcuk University Scientific Research Projects Coordinating Office (BAP) for supporting this project financially (Project No: 10401050).en_US
dc.identifier.endpage409en_US
dc.identifier.issn1733-1331en_US
dc.identifier.issn2544-4646en_US
dc.identifier.issue4en_US
dc.identifier.pmid24730135en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage401en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29287
dc.identifier.volume62en_US
dc.identifier.wosWOS:000329261400010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPOLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTSen_US
dc.relation.ispartofPOLISH JOURNAL OF MICROBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectantibiotic resistanceen_US
dc.subjectESBLen_US
dc.subjectE-test methoden_US
dc.subjectRAPD typingen_US
dc.titleCharacterization of Klebsiella pneumoniae Strains Isolated from Urinary Tract infections: Detection of ESBL Characteristics, Antibiotic Susceptibility and RAPD Genotypingen_US
dc.typeArticleen_US

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