Immunohistochemical Distribution of Heat Shock Protein 70 and Proliferating Cell Nuclear Antigen in Mouse Placenta at Different Gestational Stages

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dc.contributor.authorOzaydin, Tugba
dc.contributor.authorSur, Emrah
dc.contributor.authorOznurlu, Yasemin
dc.contributor.authorCelik, Ilhami
dc.contributor.authorUluisik, Deniz
dc.date.accessioned2020-03-26T19:24:37Z
dc.date.available2020-03-26T19:24:37Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of the present study was to investigate immunohistochemical distribution of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the mouse placenta at different gestational stages. For this purpose a total of 18 Swiss albino female mice at 12-14 weeks of age were used. Females were sacrificed on days 3 (early), 10 (mid-), and 17 (late) of pregnancy and the implantation sites of the pregnant uterus were sampled. The sections were made transversely through the central region of the implantation site and stained with hematoxylin and eosin for histological examination. PCNA and Hsp70 was stained immunohistochemically. Since the definitive placenta was not still formed on day 3 of pregnancy, Hsp70 and PCNA positivity were evaluated in only luminal epithelium and decidual-stromal cells. On days 10 and 17 of pregnancy, Hsp70 and PCNA positivity were evaluated in labyrinth zone, junctional zone and decidual layer of placenta. Hsp70 expression was observed trophoblast cells and decidual cells and was relatively constant throughout the pregnancy. This protein was strongly labeled in the trophoblast cells; while decidual cells were displayed moderate staining. In early pregnant mouse uteri, PCNA was mainly localized in decidual-stromal cells. The trophoblast cells and decidual cells displayed highly proliferative activity at the midgestational period. However there was a significant decrease in the percentage of PCNA positive cells in late gestation. (C) 2016 Wiley Periodicals, Inc.en_US
dc.identifier.doi10.1002/jemt.22624en_US
dc.identifier.endpage257en_US
dc.identifier.issn1059-910Xen_US
dc.identifier.issn1097-0029en_US
dc.identifier.issue4en_US
dc.identifier.pmid26799792en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage251en_US
dc.identifier.urihttps://dx.doi.org/10.1002/jemt.22624
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33693
dc.identifier.volume79en_US
dc.identifier.wosWOS:000372916200002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofMICROSCOPY RESEARCH AND TECHNIQUEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectdecidualizationen_US
dc.subjectplacentationen_US
dc.subjectmouseen_US
dc.subjectHsp70en_US
dc.subjectPCNAen_US
dc.titleImmunohistochemical Distribution of Heat Shock Protein 70 and Proliferating Cell Nuclear Antigen in Mouse Placenta at Different Gestational Stagesen_US
dc.typeArticleen_US

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