Determination of immunoreactive proteins of Babesia ovis

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dc.contributor.authorSevinc, Ferda
dc.contributor.authorGuler, Leyla
dc.contributor.authorSevinc, Mutlu
dc.contributor.authorEkici, Ozlem Derinbay
dc.contributor.authorIsik, Nermin
dc.date.accessioned2020-03-26T18:41:28Z
dc.date.available2020-03-26T18:41:28Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBabesia ovis, an intraerythrocytic protozoan parasite transmitted by ticks, causes severe infections in sheep in tropical and subtropical regions of the world. Parasite-specific immunoreactive proteins have been used as antigen in the serological diagnosis of babesiosis. There is no study about determination of B. ovis-specific proteins in sheep. This study was planned to determine the immunoreactive proteins of B. ovis. In this study, two splenectomized lambs, and twelve seropositive sheep and five seronegative lambs for anti-B. ovis antibodies were used as materials. Infected blood samples at 5% of parasitemia from the two splenectomized lambs experimentally infected with a virulent B. ovis field strain were analyzed for B. ovis-specific proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting (WB). B. ovis-specific five major proteins were recognized by anti-B. ovis serum but not by healthy sheep serum. They were of approximate molecular weights 154, 109, 77, 58, and 38 kDa. As the control samples, protein profiles of the blood extracts of two lambs before splenectomy operation were also blotted with the immune sera, but none of the five proteins was detected. These proteins were also immunoblotted with heterologous positive and negative sheep sera. All of twelve positive sera recognized the 109 kDa protein with 100 percent sensitivity. The 77 kDa protein reacted in 11 of 12 sera (91.6%). The sensitivities of the other 3 proteins ranged between 83.3% and 25%. The five protein bands immunoblotted with sera of the 5 negative lambs did not give any positive reaction. The results of this study revealed the presence of proteins recognized by the serum antibodies of experimentally, and naturally infected sheep with B. ovis. Additional studies on the purification of these proteins and on subsequently their utilization in a serodiagnostic method are required to improve the serological diagnosis of ovine babesiosis. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111O459]en_US
dc.description.sponsorshipThe authors would like to thank to The Scientific and Technological Research Council of Turkey for financial support (Project No: 111O459).en_US
dc.identifier.doi10.1016/j.vetpar.2013.09.012en_US
dc.identifier.endpage395en_US
dc.identifier.issn0304-4017en_US
dc.identifier.issn1873-2550en_US
dc.identifier.issue03.04.2020en_US
dc.identifier.pmid24209710en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage391en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.vetpar.2013.09.012
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29368
dc.identifier.volume198en_US
dc.identifier.wosWOS:000330087400017en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofVETERINARY PARASITOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBabesia ovisen_US
dc.subjectImmunoreactive proteinsen_US
dc.subjectSOS-PAGEen_US
dc.subjectWestern Blottingen_US
dc.titleDetermination of immunoreactive proteins of Babesia ovisen_US
dc.typeArticleen_US

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