A new immunoreactive recombinant protein designated as rBoSA2 from Babesia ovis: Its molecular characterization, subcellular localization and antibody recognition by infected sheep

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dc.contributor.authorSevinc, Ferda
dc.contributor.authorCao, Shinuo
dc.contributor.authorZhou, Mo
dc.contributor.authorSevinc, Mutlu
dc.contributor.authorCeylan, Onur
dc.contributor.authorXuan, Xuenan
dc.date.accessioned2020-03-26T19:00:28Z
dc.date.available2020-03-26T19:00:28Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractOvine babesiosis, caused by the intra-erythrocytic protozoan parasite Babesia ovis, is an infectious and economically important tick-borne disease of sheep. Diagnostic testing is an essential tool used for the control of the disease. In order to identify and characterize the immunoreactive proteins which are useful in serological diagnosis of the disease, a complementary DNA (cDNA) expression library was constructed from B. ovis merozoite mRNA. A cDNA clone designated as BoSA2 was identified by immunoscreening of a cDNA library using immune sheep serum. The sequence of the BoSA2 cDNA had a partial open reading frame of 1156 nucleotides encoding a polypeptide of 384 amino acid residues. Theoretical molecular mass for the mature protein was 43.5 kDa. The sequence of the BoSA2 was inserted into the expression vector pGEX-4T-1 and then expressed in Escherichia coli DH5 alpha cells as a glutathione S-transferase (GST)-tagged fusion protein. This recombinant fusion protein (rBoSA2) was purified by GST-affinity chromatography. Immunoreactivity of the rBoSA2 was evaluated by indirect enzyme-linked immunosorbent assay (ELISA) using the sera from the animals naturally and experimentally infected with B. ovis. ELISA results demonstrated that this antigen was useful for the diagnosis of ovine babesiosis. The localization of the BoSA2 protein was shown in and on the parasite and in the cytoplasm of the infected erythrocyte by confocal laser microscope. To our knowledge, rBoSA2 is the second immunoreactive recombinant protein of B. ovis until the present. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-113-O-336]en_US
dc.description.sponsorshipThis work received financial support from The Scientific and Technological Research Council of Turkey (TUBITAK), TOVAG-113-O-336.en_US
dc.identifier.doi10.1016/j.vetpar.2015.09.022en_US
dc.identifier.endpage218en_US
dc.identifier.issn0304-4017en_US
dc.identifier.issn1873-2550en_US
dc.identifier.issue01.02.2020en_US
dc.identifier.pmid26428018en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage213en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.vetpar.2015.09.022
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31775
dc.identifier.volume214en_US
dc.identifier.wosWOS:000366765100037en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofVETERINARY PARASITOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBabesia ovisen_US
dc.subjectcDNA libraryen_US
dc.subjectImmunoscreeningen_US
dc.subjectrBoSA2en_US
dc.subjectELISAen_US
dc.titleA new immunoreactive recombinant protein designated as rBoSA2 from Babesia ovis: Its molecular characterization, subcellular localization and antibody recognition by infected sheepen_US
dc.typeArticleen_US

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