Synthesis and characterization of cyclodextrin-based polymers as a support for immobilization of Candida rugosa lipase
Küçük Resim Yok
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The objective of this study was to prepare cross-linked beta-cyclodextrin polymers for immobilization of Candida rugosa lipase. The structures of synthesized macrocyclic compounds were characterized by Fourier transform infrared spectroscopy (FTIR). thermal gravimetric analysis (TGA) and scanning electron microscope (SEM) techniques. Properties of the immobilized systems were assessed and their performance on hydrolytic reaction were evaluated and compared with the free enzyme. The influence of activation agents (glutaraldehyde (GA) and hexamethylene diisocyanate (HMDI)) and thermal and pH stabilities of the biocatalyst was evaluated. After the optimization of immobilization process, the physical and chemical characterization of immobilized lipase was performed. Obtained data showed that the immobilized enzyme seemed better and offered some advantages in comparison with free enzyme. It can be observed that the free lipase loses its initial activity within around 80 min at 60 degrees C, while the immobilized lipases retain their initial activities of about 56% by HMDI and 82% by GA after 120 min of heat treatment at 60 degrees C. Results showed that the specific activity of the immobilized lipase with glutaraldehyde was 62.75 U/mg protein, which is 28.13 times higher than that of the immobilized lipase with HMDI. (c) 2008 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Enzyme immobilization, Candida rugosa lipase, beta-Cyclodextrin, Covalent bonding
Kaynak
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
57
Sayı
01.04.2020
