Synthesis and characterization of cyclodextrin-based polymers as a support for immobilization of Candida rugosa lipase

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dc.contributor.authorOzmen, Elif Yilmaz
dc.contributor.authorSezgin, Mehmet
dc.contributor.authorYilmaz, Mustafa
dc.date.accessioned2020-03-26T17:40:20Z
dc.date.available2020-03-26T17:40:20Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe objective of this study was to prepare cross-linked beta-cyclodextrin polymers for immobilization of Candida rugosa lipase. The structures of synthesized macrocyclic compounds were characterized by Fourier transform infrared spectroscopy (FTIR). thermal gravimetric analysis (TGA) and scanning electron microscope (SEM) techniques. Properties of the immobilized systems were assessed and their performance on hydrolytic reaction were evaluated and compared with the free enzyme. The influence of activation agents (glutaraldehyde (GA) and hexamethylene diisocyanate (HMDI)) and thermal and pH stabilities of the biocatalyst was evaluated. After the optimization of immobilization process, the physical and chemical characterization of immobilized lipase was performed. Obtained data showed that the immobilized enzyme seemed better and offered some advantages in comparison with free enzyme. It can be observed that the free lipase loses its initial activity within around 80 min at 60 degrees C, while the immobilized lipases retain their initial activities of about 56% by HMDI and 82% by GA after 120 min of heat treatment at 60 degrees C. Results showed that the specific activity of the immobilized lipase with glutaraldehyde was 62.75 U/mg protein, which is 28.13 times higher than that of the immobilized lipase with HMDI. (c) 2008 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThe Research Foundation of Selcuk UniversitySelcuk University; The Scientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106T435]en_US
dc.description.sponsorshipI would like to thank The Research Foundation of Selcuk University (BAP) and The Scientific and Technical Research Council of Turkey (TUBITAK-Grant Number 106T435) for financial support of this worken_US
dc.identifier.doi10.1016/j.molcatb.2008.07.014en_US
dc.identifier.endpage114en_US
dc.identifier.issn1381-1177en_US
dc.identifier.issn1873-3158en_US
dc.identifier.issue01.04.2020en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage109en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.molcatb.2008.07.014
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23886
dc.identifier.volume57en_US
dc.identifier.wosWOS:000264899000019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATICen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectbeta-Cyclodextrinen_US
dc.subjectCovalent bondingen_US
dc.titleSynthesis and characterization of cyclodextrin-based polymers as a support for immobilization of Candida rugosa lipaseen_US
dc.typeArticleen_US

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