DNA double-strand breaks caused by new and contemporary endodontic sealers

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dc.contributor.authorEldeniz, A. U.
dc.contributor.authorShehata, M.
dc.contributor.authorHoegg, C.
dc.contributor.authorReichl, F. X.
dc.date.accessioned2020-03-26T19:23:38Z
dc.date.available2020-03-26T19:23:38Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAimTo investigate the cytotoxicity and genotoxicity of a new silicate-based BioRoot RCS (R) sealer in comparison with contemporary sealers. MethodologyA periodontal ligament cell line using lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) was used and exposed to subtoxic concentrations of 24-h eluates from two epoxy resin-based (AH Plus Jet((R)) and Acroseal((R))), four various methacrylate-based endodontic sealers (EndoREZ((R)), RealSeal((R)), RealSeal SE (R) and Hybrid Root SEAL((R))) and three silicate-based sealers (BioRoot RCS (R), iRootSP((R)) and MTA Fillapex((R))). The XTT-based cell viability assay was used for cytotoxicity screening of materials. The -H2AX assay was used for genotoxicity screening. In the -H2AX immunofluorescence assay, PDL-hTERT cells were exposed to eluates of the substances for 6h and DNA double-strand breaks (DSB) were detected microscopically. Induced foci represented DSBs, which can induce ATM-dependent phosphorylation of the histone H2AX. The statistical significance of the differences between the experimental groups was compared using the Student's t-test (P<0.05). ResultsThe cytotoxicity of the 24-h eluates could be ranked in the following order: Hybrid Root SEAL((R))>RealSeal((R))>Acroseal((R))>RealSeal SE (R) AH Plus Jet((R))> EndoREZ((R))>MTA Fillapex((R))> iRoot SP (R)>BioRoot RCS (R). In negative controls (cells which received medium only) 4.080.53 DSB foci (mean +/- SEM) whilst in positive controls 10.76 +/- 4.05DSB foci/cell were found. BioRoot RCS (R) and RealSeal SE (R) exhibited significant differences in foci formation at 1/3 EC50 compared with their 1/10 EC50 concentration (P<0.05). Both concentrations (1/10 and 1/3 of EC50) of AH Plus Jet((R)), Acroseal((R)), RealSeal((R)) and MTA Fillapex((R)) sealers were not significantly different when compared with the medium control (P<0.05). ConclusionsNew BioRoot RCS (R) was not toxic whilst Hybrid Root SEAL((R)) demonstrated more toxicity and DNA double-strand breaks when compared with other resin- and silicate-based root canal sealers.en_US
dc.description.sponsorshipFGD-Germany [05/2010]en_US
dc.description.sponsorshipThis study was supported by FGD-Germany [Research Project Nr. 05/2010]. The authors thank the respective manufacturers for generously providing the endodontic sealers, PD Dr. Denitsa Docheva for donating the PDL-hTERT cell line, Stefan Schulz for the technical support. [Correction added on 10 February 2016 after first online publication: the "Acknowledgements' section has been modified.].en_US
dc.identifier.doi10.1111/iej.12577en_US
dc.identifier.endpage1151en_US
dc.identifier.issn0143-2885en_US
dc.identifier.issn1365-2591en_US
dc.identifier.issue12en_US
dc.identifier.pmid26574345en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1141en_US
dc.identifier.urihttps://dx.doi.org/10.1111/iej.12577
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33454
dc.identifier.volume49en_US
dc.identifier.wosWOS:000387661000005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofINTERNATIONAL ENDODONTIC JOURNALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectcytotoxicityen_US
dc.subjectDNA double-strand breaksen_US
dc.subjecteluatesen_US
dc.subjectPDL-hTERT cellsen_US
dc.subjectsealersen_US
dc.titleDNA double-strand breaks caused by new and contemporary endodontic sealersen_US
dc.typeArticleen_US

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