New potential humic acid stationary phase toward drug components: Development of a chemometric-assisted RP-HPLC method for the determination of paracetamol and caffeine in tablet formulations

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dc.contributor.authorTopkafa, Mustafa
dc.contributor.authorAyyildiz, Hamide Filiz
dc.contributor.authorMemon, Fakhar N.
dc.contributor.authorKara, Huseyin
dc.date.accessioned2020-03-26T19:25:19Z
dc.date.available2020-03-26T19:25:19Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA new humic acid based stationary phase has been used, for the first time, to achieve the separation and quantification of paracetamol and caffeine in pharmaceutical preparations under reversed-phase high-performance liquid chromatography conditions. Central composite design was applied as a powerful tool to optimize the most dominant parameters that influence the resolution of reversed-phase high-performance liquid chromatography, that is, mobile phase composition (acetonitrile percentage in water), flow rate, and column temperature. The optimum conditions were obtained as 21.69%, 1.5 mL/min, and 15 degrees C, respectively, with the aid of a second-order quadratic model and desirability function. Under the optimum conditions, the peaks could be baseline separated within 10 min. For the developed reversed-phase high-performance liquid chromatography method, the linearity was investigated in the concentration ranges of 2-160 mg/mL (R-2 = 0.999) for paracetamol and 2-9.9 mg/mL (R-2 = 0.991) for caffeine. Mean recoveries for paracetamol and caffeine were 95.90 and 95.68%, respectively. The limits of detection and quantification were 4.1 x 10(-4) and 1.3 x 10(-3) mg/mL for paracetamol and 1.6 x 10(-4) and 5.0 x 10(-4) mg/mL for caffeine. The results showed that the new humic acid based stationary phase is very suitable for the separation of paracetamol and caffeine in pharmaceutical preparations and, thus it can be used effectively in the pharmaceutical industry.en_US
dc.description.sponsorshipSelcuk University Coordinators of Scientific ResearchSelcuk University; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis study was supported by Selcuk University Coordinators of Scientific Research. The authors wish to thank the principal of Selcuk University and Scientific Research Projects Coordination. Authors would like to thank Scientific and Technological Research Council of Turkey (TUBITAK) for financial support to Dr. Fakhar-un-Nisa Memon, Post-doc. fellow under 2216 program in Selcuk University, Faculty of Science, Department of Chemistry, Konya, Turkey.en_US
dc.identifier.doi10.1002/jssc.201600045en_US
dc.identifier.endpage2458en_US
dc.identifier.issn1615-9306en_US
dc.identifier.issn1615-9314en_US
dc.identifier.issue13en_US
dc.identifier.pmid27197723en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2451en_US
dc.identifier.urihttps://dx.doi.org/10.1002/jssc.201600045
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33825
dc.identifier.volume39en_US
dc.identifier.wosWOS:000379905600002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEY-V C H VERLAG GMBHen_US
dc.relation.ispartofJOURNAL OF SEPARATION SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCaffeineen_US
dc.subjectCentral composite designen_US
dc.subjectImmobilized humic aciden_US
dc.subjectParacetamolen_US
dc.subjectReversed-phase high-performance liquid chromatographyen_US
dc.titleNew potential humic acid stationary phase toward drug components: Development of a chemometric-assisted RP-HPLC method for the determination of paracetamol and caffeine in tablet formulationsen_US
dc.typeArticleen_US

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