A new approach to chromosomal abnormalities in sperm from patients with oligoasthenoteratozoospermia: detection of double aneuploidy in addition to single aneuploidy and diploidy by five-color fluorescence in situ hybridization using one probe set

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dc.contributor.authorDurakbasi-Dursun, Hatice Gul
dc.contributor.authorZamani, Ayse Gul
dc.contributor.authorKutlu, Ruhusen
dc.contributor.authorGorkemli, Huseyin
dc.contributor.authorBahce, Muhterem
dc.contributor.authorAcar, Aynur
dc.date.accessioned2020-03-26T17:26:19Z
dc.date.available2020-03-26T17:26:19Z
dc.date.issued2008
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective: To determine the frequencies of disomy, nullisomy, total aneuploidy, and diploidy in the sperms of infertile men. Design: A controlled prospective study. Setting: Assisted reproductive technology (ART)/IVF Unit and Department of Medical Biology and Genetics, Meram Medical Faculty, Konya, Turkey. Patient(s): Infertile men with oligoasthenoteratozoospermia (OAT) and normal fertile donors. Intervention(s): After slide preparation from semen samples, sperm nuclei were analyzed for chromosomes 13, 18, 21, X, and Y by five-color fluorescence in situ hybridization. Main Outcome Measure(3): The sperm aneuploidy (disomy and nullisomy) and diploidy rates were determined according to the number of signals detected for each probe in infertile and fertile men. Results: Patients with OAT had a significantly higher incidence of disomy (except chromosome 18 and XX disomy), nullisomy (except chromosome 18), and diploidy than normal fertile controls. In addition to double disomy, double nullisomy and disomy+nullisomy were observed in patients with OAT, but none of these were seen in controls. Conclusion(s): In this study patients with OAT had an increased rate of sperm aneuploidy and diploidy. This finding suggest that patients with OAT may be at an increased risk of producing aneuploid and triploid offsprings. For this reason, it may be very, important to perform the sperm fluorescence in situ hybridization in patients with OAT. Thus, a more informative genetic counseling might be given to couples with male factor infertility who are at an increased risk of having Aneuploid offsprings and triploid conceptions before intracytoplasmic sperm injection (ICSI). (Fertil Steril (R) 2008;89:1709-17. (c) 2008 by American Society for Reproductive Medicine.).en_US
dc.identifier.doi10.1016/j.fertnstert.2007.06.050en_US
dc.identifier.endpage1717en_US
dc.identifier.issn0015-0282en_US
dc.identifier.issn1556-5653en_US
dc.identifier.issue6en_US
dc.identifier.pmid18036525en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1709en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.fertnstert.2007.06.050
dc.identifier.urihttps://hdl.handle.net/20.500.12395/22160
dc.identifier.volume89en_US
dc.identifier.wosWOS:000256892600021en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.ispartofFERTILITY AND STERILITYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectmale infertilityen_US
dc.subjectsperm FISHen_US
dc.subjectoligoasthenoteratozoospermiaen_US
dc.titleA new approach to chromosomal abnormalities in sperm from patients with oligoasthenoteratozoospermia: detection of double aneuploidy in addition to single aneuploidy and diploidy by five-color fluorescence in situ hybridization using one probe seten_US
dc.typeArticleen_US

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