Effects of dithioerythritol on ram semen after the freeze-thawing process

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dc.contributor.authorBaspinar, Nuri
dc.contributor.authorCoyan, Kenan
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorTuncer, Purhan Barbaros
dc.date.accessioned2020-03-26T18:14:22Z
dc.date.available2020-03-26T18:14:22Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to evaluate the effects of dithioerythritol added to cryopreservation extender on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2 years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing 0.5, 1, and 2 mM dithiothreitol and no antioxidant (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws. Frozen straws were then thawed individually at 37 degrees C for 20s in a water bath for evaluation. The addition of dithioerythritol at 0.5 and 2 mM doses led to higher percentages of subjective motility (62.9 +/- 4.2% and 63.6 +/- 1.8%) compared to control (52.0 +/- 4.9%, P < 0.05). As regards CASA motility, dithioerythritol 0.25 and 2 mM (60.2 +/- 4.5% and 59.6 +/- 1.2%) groups were higher from that of control (44.2 +/- 8.7%, P < 0.05). For the CASA progressive motility, 0.25, 0.5 and 2 mM doses of dithioerythritol (22.0 +/- 2.1%, 21.7 +/- 2.5% and 24.0 +/- 1.2%) had increasing effect in comparison to control (15.0 +/- 2.5%). Dithioerythritol at 1 and 2 mM doses for ALH provided higher values compared to the control (P < 0.001) following the freeze-thawing process. Supplementation with dithiothreitol did not significantly affect the integrities of sperm membrane and acrosome, and mitochondrial activities. No significant differences were observed in biochemical parameters among the groups (P > 0.05). Findings of this study showed that dithioerythritol supplementation in semen extenders, was of greater benefit to sperm motility of frozen-thawed ram sperm. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipSelcuk University, Directorate of Scientific Research Projects (BAP)Selcuk University; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108 O 522]en_US
dc.description.sponsorshipThis study was financed under the project supported by Selcuk University, Directorate of Scientific Research Projects (BAP) and by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 108 O 522).en_US
dc.identifier.doi10.1016/j.cryobiol.2011.08.004en_US
dc.identifier.endpage156en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issue3en_US
dc.identifier.pmid21945818en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage152en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2011.08.004
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26402
dc.identifier.volume63en_US
dc.identifier.wosWOS:000297907800005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRam spermen_US
dc.subjectDithioerythritolen_US
dc.subjectSperm motilityen_US
dc.subjectFreezing-thawingen_US
dc.titleEffects of dithioerythritol on ram semen after the freeze-thawing processen_US
dc.typeArticleen_US

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