In Vitro Analysis of the Co-Assembly of Type-I and Type-III Collagen

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dc.contributor.authorEryilmaz, Esma
dc.contributor.authorTeizer, Winfried
dc.contributor.authorHwang, Wonmuk
dc.date.accessioned2020-03-26T19:41:53Z
dc.date.available2020-03-26T19:41:53Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAn important step towards achieving functional diversity of biomimetic surfaces is to better understand the co-assembly of the extracellular matrix components. For this, we study type-I and type-III collagen, the two major collagen types in the extracellular matrix. By using atomic force microscopy, custom image analysis, and kinetic modeling, we study their homotypic and heterotypic assembly. We find that the growth rate and thickness of heterotypic fibrils decrease as the fraction of type-III collagen increases, but the fibril nucleation rate is maximal at an intermediate fraction of type-III. This is because the more hydrophobic type-I collagen nucleates fast and grows in both longitudinal and lateral directions, whereas more hydrophilic type-III limits lateral growth of fibrils, driving more monomers to nucleate additional fibrils. This demonstrates that subtle differences in physico-chemical properties of similar molecules can be used to fine-tune their assembly behavior.en_US
dc.description.sponsorshipTurkish Ministry of Education fellowshipen_US
dc.description.sponsorshipWe acknowledge using the Texas A & M Materials Characterization Facility for part of the experiments. Esma Eryilmaz was supported by the Turkish Ministry of Education fellowship.en_US
dc.identifier.doi10.1007/s12195-016-0466-3en_US
dc.identifier.endpage53en_US
dc.identifier.issn1865-5025en_US
dc.identifier.issn1865-5033en_US
dc.identifier.issue1en_US
dc.identifier.pmid31719849en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage41en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s12195-016-0466-3
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35196
dc.identifier.volume10en_US
dc.identifier.wosWOS:000394178200005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofCELLULAR AND MOLECULAR BIOENGINEERINGen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAFMen_US
dc.subjectBiomimetic surfaceen_US
dc.subjectCollagenen_US
dc.subjectExtracellular matrixen_US
dc.subjectHeterogeneous assemblyen_US
dc.subjectMacromolecular assemblyen_US
dc.titleIn Vitro Analysis of the Co-Assembly of Type-I and Type-III Collagenen_US
dc.typeArticleen_US

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