The response of cementoblasts to calcium phosphate resin-based and calcium silicate-based commercial sealers

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dc.contributor.authorHakki, S. S.
dc.contributor.authorBozkurt, B. S.
dc.contributor.authorOzcopur, B.
dc.contributor.authorGandolfi, M. G.
dc.contributor.authorPrati, C.
dc.contributor.authorBelli, S.
dc.date.accessioned2020-03-26T18:44:09Z
dc.date.available2020-03-26T18:44:09Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractHakki SS, Bozkurt BS, Ozcopur B, Gandolfi MG, Prati C, Belli S. The response of cementoblasts to calcium phosphate resin-based and calcium silicate-based commercial sealers. International Endodontic Journal, 46, 242-252, 2013. Aim To investigate cell viability and gene expression of cementoblasts (OCCM.30) exposed to extractable components released by resin-based sealers with different chemical composition Hybrid Root Seal (HRS), SimpliSeal (SS), Real Seal (RS) and AH Plus (AH) and by a MTA-based sealers Tech Biosealer Endo (TBE). Methodology Discs of all materials were prepared and allowed to set in humid conditions at 37 degrees for 48h. The discs were then incubated for 72h at 37 degrees C to obtain material extracts (1/1) in DMEM. The extracts containing the components released by the sealers were filtered and other dilutions (1/2, 1/4) were prepared from the original solution (1/1). Original and diluted solutions were tested on the cementoblasts. Impedance-based real-time cell analysis (RTCA) was used to evaluate cell viability, quantitative real-time polymerase chain reaction (QRT-PCR) was used to examine the expression of mineralization-related genes (osteocalcin; OCN, Runt-related transcription factor-2; Runx2, collagen type 1; COL I, alkaline phosphatase; ALP). For statistical analysis, one-way analysis of variance (anova) and Tukey's honestly significant difference (HSD) tests were used. Results TBE (1/2), RS (1/2, 1/4), and HRS (1/2, 1/4) significantly decreased cell viability (P<0.001). AH (1/2, 1/4) and SS (1/2, 1/4) had similar cell viability to the control at 30h. All tested materials significantly decreased cell viability when compared to the control group except AH (1/2, 1/4) and SS (1/4) at 90h. All of the tested sealers reduced COL I mRNA expressions when compared to the control. SS was associated with significant increases in OCN and Runx2 mRNA expressions when compared to the control (P<0.001). Whereas all of the dilutions of TBE, RS and HRS significantly decreased BSP mRNA expressions (P<0,001), 1/2 and 1/4 dilutions of SS increased BSP mRNA expression (P<0,001). Except the 1/4 dilutions of AH and SS, all the sealer dilutions significantly reduced ALP mRNA expression in cementoblasts (P<0,001). Conclusions SimpliSeal and AH Plus resulted in more favourable response to cementoblasts because of their regulation potential on the mineralized tissue-associated protein's mRNA expressions.en_US
dc.description.sponsorshipResearch Projects Center of Selcuk University (BAP) Konya, TurkeySelcuk Universityen_US
dc.description.sponsorshipThe authors deny any conflicts of interest. This study was supported in part by Research Projects Center of Selcuk University (BAP) Konya, Turkey. The materials are generously supplied by the companies.en_US
dc.identifier.doi10.1111/j.1365-2591.2012.02122.xen_US
dc.identifier.endpage252en_US
dc.identifier.issn0143-2885en_US
dc.identifier.issue3en_US
dc.identifier.pmid23005923en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage242en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1365-2591.2012.02122.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29951
dc.identifier.volume46en_US
dc.identifier.wosWOS:000314922000008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEY-BLACKWELLen_US
dc.relation.ispartofINTERNATIONAL ENDODONTIC JOURNALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectcalcium fluoro silicate cementen_US
dc.subjectcell proliferationen_US
dc.subjectcell viabilityen_US
dc.subjectcytotoxicityen_US
dc.subjectendodonticsen_US
dc.subjectmRNAen_US
dc.titleThe response of cementoblasts to calcium phosphate resin-based and calcium silicate-based commercial sealersen_US
dc.typeArticleen_US

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