Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro

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dc.contributor.authorHakki, Sema S.
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorTuerkay, Emre
dc.contributor.authorDard, Michel
dc.contributor.authorPurali, Nuhan
dc.contributor.authorGoetz, Werner
dc.date.accessioned2020-03-26T19:55:27Z
dc.date.available2020-03-26T19:55:27Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAmelogenin (AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG (rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts (OCCM-30) were treated with different concentrations (0.1, 1, 10, 100, 1000, 10,000, 100,000 ng . mL(-1)) of recombinant human AMG (rhAMG) and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type I (COL I), osteopontin (OPN), runt-related transcription factor 2 (Runx2), cementum attachment protein (CAP), and alkaline phosphatase (ALP) genes using quantitative RT-PCR. The dose response of rhAMG was evaluated using a real-time cell analyzer. Total RNA was isolated on day 3, and cell mineralization was assessed using von Kossa staining on day 8. COL I, OPN and lysosomal-associated membrane protein-1 (LAMP-1), which is a cell surface binding site for amelogenin, were evaluated using immunocytochemistry. F-actin bundles were imaged using confocal microscopy. rhAMG at a concentration of 100,000 ng . mL(-1) increased cell proliferation after 72 h compared to the other concentrations and the untreated control group. rhAMG (100,000 ng . mL(-1)) upregulated BSP and OCN mRNA expression levels eightfold and fivefold, respectively. rhAMG at a concentration of 100,000 ng . mL(-1) remarkably enhanced LAMP-1 staining in cementoblasts. Increased numbers of mineralized nodules were observed at concentrations of 10,000 and 100,000 ng . mL(-1) rhAMG. The present data suggest that rhAMG is a potent regulator of gene expression in cementoblasts and support the potential application of rhAMG in therapies aimed at fast regeneration of damaged periodontal tissue.en_US
dc.description.sponsorshipTUBITAK (Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG108S265]; BMBF (Germany)Federal Ministry of Education & Research (BMBF) [TUR08/09]en_US
dc.description.sponsorshipThis study was supported by TUBITAK SBAG108S265 (Turkey) and BMBF TUR08/09 (Germany). We thank Johan Svensson and Leif Bulow for rhAMG production and characterization studies from Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering Lund University, Lund, Sweden for rhAMG synthesis.en_US
dc.identifier.citation
dc.identifier.doi10.1038/s41368-018-0010-5en_US
dc.identifier.endpage10en_US
dc.identifier.issn1674-2818en_US
dc.identifier.issn2049-3169en_US
dc.identifier.pmid29748557en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://dx.doi.org/10.1038/s41368-018-0010-5
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36910
dc.identifier.volume10en_US
dc.identifier.wosWOS:000431796100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthor
dc.institutionauthor
dc.language.isoenen_US
dc.publisherNATURE PUBLISHING GROUPen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF ORAL SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleRecombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitroen_US
dc.typeArticleen_US

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