Implications from a pharmacogenomic analysis: Nerium oleander leaf distillate supplemented diet regulates cholesterol metabolism in rats

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dc.contributor.authorKars, Meltem Demirel
dc.contributor.authorOdabasi, Burcu Asena
dc.contributor.authorKars, Gokhan
dc.contributor.authorUney, Kamil
dc.contributor.authorBagci, Yavuz
dc.contributor.authorBas, Ahmet Levent
dc.date.accessioned2020-03-26T18:51:03Z
dc.date.available2020-03-26T18:51:03Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractContext: Despite the usage of Nerium oleander L. (Apocynaceae) for anticancer studies and traditional remediation, the regulatory effect of N. oleander leaf distillate on cholesterol metabolism is not disclosed sufficiently. Objective: Cholesterol is an important biological molecule and the synthesis rate is regulated by the amount of cholesterol uptake from the diet. The aim of this study was to investigate the regulation of cholesterol metabolism in response to a high-fat diet (HFD) and the effects of N. oleander leaf distillate-supplemented diet (NOHFD) in rats. Materials and methods: Microarray technology was used to clarify the regulation of cholesterol mechanism in HFD and NOHFD-fed rats (375 mu g/0.5 mL distilled water applied by gavage). The treatment period was 90 days. Rat liver tissues were used for microarray analysis using the Affymetrix GeneChip Rat Genome platform. Results of groups were statistically analyzed with the Partek 6.6 bioinformatic program. Results: The HFD group exhibited alterations in the expression levels of about 1945 genes with respect to the normal diet (ND) group. The results showed that expression levels of 47 genes were altered related to cholesterol metabolism in HFD and NOHFD groups. The expression levels of seven genes in the NOHFD group were significantly closer to those in the ND group than those of the HFD group. Discussion and conclusion: To conclude, findings suggest that N. oleander leaf distillate-supplemented food has considerable beneficial effects on cholesterol metabolism-related gene expression levels.en_US
dc.description.sponsorshipSelcuk University Research FundSelcuk University [12201031]en_US
dc.description.sponsorshipAll authors fully disclose that there is no financial or ethical conflict of interest. This study was supported by the Selcuk University Research Fund with the project number 12201031. Also, the distillation method was submitted to Turkish Patent Institute (Application No: 2009/00312) and Patent Cooperation Treaty (Application No: PCT/TR2009/000013).en_US
dc.identifier.doi10.3109/13880209.2013.874535en_US
dc.identifier.endpage993en_US
dc.identifier.issn1388-0209en_US
dc.identifier.issn1744-5116en_US
dc.identifier.issue8en_US
dc.identifier.pmid24617822en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage988en_US
dc.identifier.urihttps://dx.doi.org/10.3109/13880209.2013.874535
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30915
dc.identifier.volume52en_US
dc.identifier.wosWOS:000340109700010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofPHARMACEUTICAL BIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHigh fat dieten_US
dc.subjectliveren_US
dc.subjectmicroarrayen_US
dc.subjectphytotherapyen_US
dc.titleImplications from a pharmacogenomic analysis: Nerium oleander leaf distillate supplemented diet regulates cholesterol metabolism in ratsen_US
dc.typeArticleen_US

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